A5040078686- DNA Repair Mechanisms
- Telomeres, Telomerase, and Senescence
- Genomics and Chromatin Dynamics
- RNA Interference and Gene Delivery
- DNA and Nucleic Acid Chemistry
- Genetics, Aging, and Longevity in Model Organisms
- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- PARP inhibition in cancer therapy
- Microbial Community Ecology and Physiology
- Chromosomal and Genetic Variations
- Methane Hydrates and Related Phenomena
- Cancer-related molecular mechanisms research
- Advanced biosensing and bioanalysis techniques
- Cancer Research and Treatments
- Nuclear Structure and Function
- Acute Lymphoblastic Leukemia research
- Enzyme Catalysis and Immobilization
- Glycosylation and Glycoproteins Research
- Fungal and yeast genetics research
- Epigenetics and DNA Methylation
- Carbohydrate Chemistry and Synthesis
- Cancer-related Molecular Pathways
- Microtubule and mitosis dynamics
- Polyomavirus and related diseases
Sun Yat-sen University
2025
Sun Yat-sen Memorial Hospital
2025
McMaster University
2015-2024
Jiangjin Central Hospital
2024
University of Kentucky
2024
Liaoning Cancer Hospital & Institute
2024
China Medical University
2024
Dalian University of Technology
2024
Agricultural Research Service
2023
Beijing Children’s Hospital
2022
Summary As important signal molecules, jasmonates (JAs) and green leaf volatiles (GLVs) play diverse roles in plant defense responses against insect pests pathogens. However, how plants employ their specific by modulating the levels of JA GLVs remains unclear. Here, we describe identification a role for rice HPL3 gene, which encodes hydroperoxide lyase (HPL), OsHPL3/CYP74B2, mediating plant‐specific responses. The loss‐of‐function mutant hpl3‐1 produced disease‐resembling lesions spreading...
The yeast homologs of the ATM and ATR DNA damage response kinases play key roles in telomerase-mediated telomere maintenance, but role ATM/ATR mammalian telomerase pathway has been less clear. Here, we demonstrate requirement for localization to telomeres elongation immortal human cells. Stalled replication forks increased recruitment an ATR-dependent manner. Furthermore, was observed upon phosphorylation shelterin component TRF1 at target site (S367). This leads loss from may therefore...
Abstract CSB, a member of the SWI2/SNF2 superfamily, is implicated in DNA double-strand break (DSB) repair. However, how it regulates this repair process poorly understood. Here we uncover that CSB interacts via its newly identified winged helix domain with RIF1, an effector 53BP1, and interaction mediates recruitment to DSBs S phase. At DSBs, remodels chromatin by evicting histones, which limits RIF1 MAD2L2 but promotes BRCA1 accumulation. The remodeling activity requires not only...
TRF2, a component of the shelterin complex, functions to protect telomeres. TRF2 contains an N-terminal basic domain rich in glycines and arginines, similar GAR motif that is methylated by protein arginine methyltransferases. However, whether methylation regulates function has not been determined. Here we report amino acid substitutions arginines with lysines induce telomere dysfunction-induced focus formation, leading induction cellular senescence. We have demonstrated cells overexpressing...
The majority of Cockayne syndrome (CS) patients carry a mutation in Syndrome group B (CSB), large nuclear protein implicated DNA repair, transcription and chromatin remodeling. However, whether CSB may play role telomere metabolism has not yet been characterized. Here, we report that physically interacts with TRF2, duplex telomeric binding essential for protection. We find localizes at small subset human telomeres it is required preventing the formation dysfunction-induced foci (TIF) CS...
Ataxia telangiectasia mutated (ATM), a PI-3 kinase essential for maintaining genomic stability, has been shown to regulate TRF1, negative mediator of telomerase-dependent telomere extension. However, little is known about ATM-mediated TRF1 phosphorylation site(s) in vivo. Here, we report that ATM phosphorylates S367 and this renders free chromatin. We show phosphorylated (pS367)TRF1 forms distinct non-telomeric subnuclear foci these occur predominantly S G2 phases, implying their formation...
A comprehensive dataset detailing protein interactors for the PARP family has been generated using TurboID proximity labeling under standardized experimental conditions. V5-TurboID fusion constructs enabled identification of 6,314 high-confidence interacting proteins through mass spectrometry, capturing transient interactions undetectable by conventional methods. Parallel GFP-PARP localization experiments validated physiological subcellular distributions. The reveals both shared and unique...
CSB, a member of the SWI2/SNF2 superfamily, has been implicated in evicting histones to promote DSB pathway choice towards homologous recombination (HR) repair. However, how CSB promotes HR repair remains poorly characterized. Here we demonstrate that interacts with both MRE11/RAD50/NBS1 (MRN) and BRCA1 cell cycle regulated manner, former requiring its WHD occurring predominantly early S phase. BRCT domain this interaction is by CDK-dependent phosphorylation on S1276. The CSB-BRCA1...
TRF1, a duplex telomeric DNA binding protein, is implicated in homologous-recombination-based alternative lengthening of telomeres, known as ALT. However, how TRF1 promotes ALT activity has yet to be fully characterized. Here we report that Cdk-dependent phosphorylation on T371 acts switch create pool referred (pT371)TRF1, which recruited ALT-associated PML bodies (APBs) S and G2 phases independently its DNA. We find essential for APB formation C-circle production, both are hallmarks show...
ABSTRACT Elevated replication stress is evident at telomeres of about 10-15% cancer cells, which maintain their via a homologous recombination (HR)-based mechanism, referred to as alternative lengthening (ALT). How ALT cells resolve support growth remains incompletely characterized. Here, we report that CSB (also known ERCC6) promotes recruitment HR repair proteins (MRN, BRCA1, BLM and RPA32) POLD3 telomeres, process requires the ATPase activity controlled by ATM- CDK2-dependent...
The FLP recombinase of the 2 microM plasmid Saccharomyces cerevisiae belongs to integrase family recombinases whose members have in common four absolutely conserved residues (Arg-191, His-305, Arg-308, and Tyr-343). We studied mutant protein R308K which arginine residue at position 308 has been replaced by lysine. Although was previously reported be defective ligation certain substrates (Pan, G., Luetke, K., Sadowski, P.D., Mol. Cell. Biol. 13, 3167-3175, 1993b), we show this work that is...
Abstract TRF1, a duplex telomeric DNA-binding protein, plays an important role in telomere metabolism. We have previously reported that fraction of endogenous TRF1 can stably exist free chromatin when it is phosphorylated at T371 by Cdk1; however, the this telomere-free (pT371)TRF1 has yet to be fully characterized. Here we show recruited sites DNA damage, forming damage-induced foci response ionizing radiation (IR), etoposide and camptothecin. find IR-induced formation dependent on ATM-...
CSB (Cockayne syndrome group B) and SMARCAL1 (SWI/SNF-related, matrix-associated, actin-dependent, regulator of chromatin, subfamily A-like 1) are DNA translocases that belong to the SNF2 helicase family. They both enriched at stalled replication forks. While is recruited by RPA32 forks, little known about whether also regulates CSB's association with Here, we report directly interacts RPA, least in part via a RPA32C-interacting motif within N-terminal region CSB. Modeling CSB-RPA32C...
The FLP recombinase of the 2-μm plasmid Saccharomyces cerevisiae belongs to integrase family whose members form a covalent bond between conserved tyrosine and 3′-phosphoryl group at site cleavage. Ligation takes place when 5′-OH generated during cleavage step attacks phosphotyrosine reforms phosphodiester bond. When incoming is from partner duplex, strand exchange occurs.The recognition target (FRT) contains two inverted 13-base pair (bp) binding sequences that surround an 8-bp core region....