A5101686541- Acute Myeloid Leukemia Research
- Epigenetics and DNA Methylation
- Hematopoietic Stem Cell Transplantation
- Hematological disorders and diagnostics
- Multiple Myeloma Research and Treatments
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Acute Lymphoblastic Leukemia research
- RNA modifications and cancer
- Immune Cell Function and Interaction
- Histone Deacetylase Inhibitors Research
- Vascular Tumors and Angiosarcomas
- Neutropenia and Cancer Infections
- Chronic Myeloid Leukemia Treatments
- Cancer-related gene regulation
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Bone and Joint Diseases
- Autoimmune and Inflammatory Disorders Research
- RNA Research and Splicing
- Cancer, Hypoxia, and Metabolism
- Pneumocystis jirovecii pneumonia detection and treatment
- Cerebrovascular and Carotid Artery Diseases
- Sarcoma Diagnosis and Treatment
- Cancer Genomics and Diagnostics
- Cancer-related molecular mechanisms research
- RNA Interference and Gene Delivery
Shanghai Sixth People's Hospital
2015-2025
Soochow University
2014-2025
Shanghai Eighth People Hospital
2023-2024
Sichuan University
2024
Center for Rheumatology
2024
West China Hospital of Sichuan University
2024
Shanghai Jiao Tong University
2014-2023
Group Sense (China)
2021
First Affiliated Hospital Zhejiang University
2020
WuXi AppTec (China)
2019
Abstract Iron overload (IO) has been reported to contribute mesenchymal stromal cell (MSC) damage, but the precise mechanism yet be clearly elucidated. In this study, we found that IO increased apoptosis and lowered viability in MSCs, accompanied by extensive mitochondrial fragmentation autophagy enhancement. All these effects were reactive oxygen species (ROS) dependent. MSCs with IO, ATP concentrations significantly reduced due high ROS levels low electron respiratory chain complex (ETC)...
To identify the molecular signatures that predict responses to decitabine (DAC), we examined baseline gene mutations (28 target genes) in 109 myelodysplastic syndrome (MDS) patients at diagnosis. We determined TP53 predicted complete response (CR), as 10 of 15 (66·7%) who possessed achieved a CR. Univariate and multivariate analyses showed are only predictive CR DAC MDS. Among ten with CR, nine presented complex karyotypes due abnormalities involving chromosome 5 and/or 7, eight monosomies....
Bone marrow stromal cells from patients with myelodysplastic syndrome (MDS) display a senescence phenotype, but the underlying mechanism has not been elucidated. Pro-inflammatory signaling within malignant clone and bone microenvironment identified as key pathogenetic driver of MDS. Our study revealed that S100A9 is highly-expressed in lower-risk Moreover, normal primary mesenchymal (MSCs) human cell line HS-27a co-cultured MDS mononuclear acquired phenotype. Exogenous supplemented also...
Abstract Aberrant self-renewal of leukemia initiation cells (LICs) drives aggressive acute myeloid (AML). Here, we report that UHRF1, an epigenetic regulator recruits DNMT1 to methylate DNA, is highly expressed in AML and predicts poor prognosis. UHRF1 required for leukemogenesis by maintaining LICs. Mechanistically, directly interacts with Sin3A-associated protein 30 (SAP30) through two critical amino acids, G572 F573 its SRA domain, repress gene expression. Depletion or SAP30 derepresses...
Abstract Purpose Diabetes mellitus (DM) is the second most common comorbidity in myelodysplastic syndromes (MDS). The purpose of study was to investigate clinical characteristics MDS patients with DM. Methods A retrospective analysis performed on data 890 or without Clinical data, including genetic changes, overall survival (OS), leukemia-free (LFS) and infection, were analyzed. Results Among patients, 184 (20.7%) had TET2 SF3B1 mutations occurred more frequently DM group than those non-DM (...
We determined the biological and prognostic significance of five recurrent genetic aberrations in Chinese patients with myelodysplastic syndromes (MDS). A total 304 MDS were screened for known mutations genes (ASXL1, U2AF1, SF3B1, SRSF2, EZH2) using next-generation sequencing. Of these, 97 (31.9 %) harbored at least one mutation genes, harboring these had distinct clinical features. Incidence ratios ASXL1, EZH2 11.8, 8.6, 8.2, 4.3, 3.6 %, respectively. Patients more commonly high-risk than...
Stroke recurrence and disability in patients with a minor stroke is one of the most depressing medical situations. In this study, we aimed to identify which factors were associated adverse outcomes stroke.The China National Registry (CNSR) nationwide prospective registry for presented hospitals acute cerebrovascular events between September 2007 August 2008. The 3-month follow-up was completed 4669 defined as initial neurological severity lower than 4 Institutes Health Scale (NIHSS)....
Myelodysplastic syndromes (MDS) are heterogeneous hematopoietic disorders that incurable with conventional therapy. Their incidence is increasing global population aging. Although many genetic, epigenetic, splicing, and metabolic aberrations have been identified in patients MDS, their clinical features quite similar. Here, we show hypoxia-independent activation of hypoxia-inducible factor 1α (HIF1A) signaling both necessary sufficient to induce dysplastic cytopenic MDS phenotypes. The HIF1A...
Abstract Novel sequencing designs are necessary to supplement the recognized knowledge of myelodysplastic syndrome (MDS)-related genomic alterations. In this study, we sequenced 28 target genes in 320 Chinese MDS patients but obtained 77.2% recall factors and 82.8% genetic abnormalities (including karyotype abnormalities). addition known relationships among mutations, some specific chromosomal were found link gene mutations. Trisomy 8 tended be linked U2AF1 ZRSR2 20q- exhibited higher...
Ribosomal protein (RP) L23 is a negative regulator of cellular apoptosis, and RPL23 overexpression associated with abnormal apoptotic resistance in CD34+ cells derived from patients higher-risk myelodysplastic syndrome (MDS). However, the mechanism underlying RPL23-induced MDS poorly understood. In this study, we showed that reduced expression led to suppressed viability, increased apoptosis G1-S cell cycle arrest. Gene microarray analysis comparing RPL23-knockdown control identified an...
Abstract The progressive mechanism underlying myelodysplastic syndrome remains unknown. Here we identify ROBO1 and ROBO2 as novel progression-related somatic mutations using whole-exome targeted sequencing in 6 of 16 (37.5%) paired MDS patients with disease progression. Further deep detects 20 (10.4%) ROBO a cohort 193 patients. In addition, copy number loss heterogeneity (LOH) are frequently observed progression or carrying mutations. vitro experiments, overexpression produces...
// Feng Xu 1, * , Li Liu Chun-Kang Chang 1 Qi He Ling-Yun Wu Zheng Zhang Wen-Hui Shi Juan Guo Yang Zhu You-Shan Zhao Shu-Cheng Gu Cheng-Ming Fei Xiao Department of Hematology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China These authors contributed equally to this work Correspondence to: Xiao, e-mail: lixiao3326@163.com Keywords: myelodysplastic syndrome, EZH2, H3K27 methylation, overexpression, HOX genes Received: August 10,...
Objective: To investigate the cytopathological features of thyroid tumor with DICER1 mutation. Methods: A retrospective study on preoperative cell smear was conducted tumors gene mutations detected by Sanger sequencing in Department Pathology Shanghai Sixth People's Hospital affiliated to Jiaotong University School Medicine from May 2022 November 2024. Results: Totally 163 cases histological indicating mutation related underwent sequencing. Fifteen were confirmed harbor (15/163,9.2%)....
Lower risk myelodysplastic syndromes (MDSs) are characterised by increased apoptosis of haematopoietic cells in the bone marrow (BM). The mechanism driving this excessive involves multiple immune molecules, including inflammatory cytokines such as interferon-γ (IFN-γ), tumour necrosis factor-α (TNF-α) and interleukins (ILs). Interleukin-17 (IL-17) is hallmark cytokine produced CD4(+) Th17 cells, IL-17 mediates activation adaptive T-cell response inducing an environment. However, little known...
Abstract Background Intestinal epithelial homeostasis is critical for maintaining gut integrity and function. Methionine an essential amino acid involved in various cellular processes, its dietary regulation may impact intestinal injury repair mechanisms. Selenbp1, oxidase of the methionine metabolite methanethiol, plays important role this process. This study investigates intake Selenbp1 colonic repair. Methods Animal models with radiation-induced were used to assess effects restriction...
Rigosertib has demonstrated therapeutic activity for patients with high-risk myelodysplastic syndrome (MDS) in clinical trials. However, the role of rigosertib MDS not been thoroughly characterized. In this study, we found out that induced apoptosis, blocked cell cycle at G2/M phase and subsequently inhibited proliferation CD34+ cells from MDS, while it minimally affected normal cells. Further studies showed acted via activation P53 signaling pathway. Bioinformatics analysis based on gene...
Abnormal immunophenotypes of haematopoietic cells in myelodysplastic syndromes (MDS) have been identified by flow cytometry (FCM) as a typical characteristic myeloid dysplasia. Considering that most MDS patients show varying degrees erythroid dysplasia, we analysed the immunophenotypic feature erythroblasts to evaluate its diagnostic application MDS.Erythroid antigens CD71 and CD105 expression were using FCM. The development index (DI) acquired log transformation CD71/CD105 ratio was used...
Abstract Mutations in the U2 small nuclear RNA auxiliary factor 1 ( U2AF1 ) gene are common feature of a major subset myelodysplastic syndromes (MDS). However, genetic landscape and molecular pathogenesis oncogenic S34F mutation MDS not totally understood. We performed comprehensive analysis for prognostic significance mutations acute myeloid leukemia (AML) cohort based on The Cancer Genome Atlas (TCGA) database. Functional was vitro. Differentially expressed genes (DEGs) significantly...
DHX9 is a member of the DEAH (Asp-Glu-Ala-His) helicase family and regulates DNA replication RNA processing. dysfunction promotes tumorigenesis in several solid cancers. However, role MDS still unknown. Here, we analyzed expression its clinical significance 120 patients 42 non-MDS controls. Lentivirus-mediated DHX9-knockdown experiments were performed to investigate biological function. We also cell functional assays, gene microarray, pharmacological intervention mechanistic involvement...
The bone marrow microenvironment (BMM) can regulate leukemia stem cells (LSCs) via secreted factors. Increasing evidence suggests that dissecting the mechanisms by which BMM maintains LSCs may lead to development of effective therapies for eradication leukemia. Inhibitor DNA binding 1 (ID1), a key transcriptional regulator in LSCs, previously identified us, controls cytokine production BMM, but role ID1 acute myeloid (AML) remains obscure. Here, we report is highly expressed patients with...