A5100655336- Oral microbiology and periodontitis research
- Antibiotic Resistance in Bacteria
- Immune Response and Inflammation
- Oral Health Pathology and Treatment
- Gout, Hyperuricemia, Uric Acid
- Oral and gingival health research
- Salivary Gland Disorders and Functions
- Hepatitis B Virus Studies
- Immune cells in cancer
- interferon and immune responses
- Vibrio bacteria research studies
- Cancer-related molecular mechanisms research
- Spectroscopy Techniques in Biomedical and Chemical Research
- Gut microbiota and health
- Aquaculture disease management and microbiota
- Bacteriophages and microbial interactions
- Liver Disease Diagnosis and Treatment
- Enterobacteriaceae and Cronobacter Research
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Diabetes and associated disorders
- Vasculitis and related conditions
- Adipokines, Inflammation, and Metabolic Diseases
- Alcohol Consumption and Health Effects
- Streptococcal Infections and Treatments
- Protease and Inhibitor Mechanisms
Guangzhou Medical University
2016-2025
Jinshan Hospital of Fudan University
2025
Fujian Medical University
2025
Fujian Provincial Hospital
2025
Southern University of Science and Technology
2025
Zunyi Medical University
2024
Southern Medical University
2016-2024
Academy of Military Medical Sciences
2024
Stomatology Hospital
2016-2023
Northwestern Polytechnical University
2022-2023
Background: Macrophages are central players in the pathogenesis of periodontitis. However, phenotypic switch macrophage M1/M2 remains uncertain. Methods: Adult male mice were divided into periodontitis (P) or control (C) groups. Bone marrow–derived macrophages (BMMs) stimulated with Porphyromonas gingivalis lipopolysaccharide (LPS). In both periodontium and serum, M1 M2 phenotypes detected vivo vitro via following: 1) immunofluorescence; 2) immunohistochemistry; 3) electrochemiluminescence...
ABSTRACT Aim To investigate the effects of hyperuricemia on periodontitis and underlying mechanisms by establishing combined animal cell models. Methods A mouse model was established potassium oxonate injection, with sodium carboxymethylcellulose treatment serving as controls. Both models were treated or without induction ( n = 10/group). RAW264.7 macrophages THP‐1‐derived stimulated Porphyromonas gingivalis ‐lipopolysaccharide in presence normal excessive concentrations uric acid....
High prevalence rates of sulfonamide resistance genes sul1, sul2 and sul3 have been observed in Gram-negative bacteria isolated from humans, domestic animals aquaculture species worldwide. We investigated the distribution characteristics, location, conjugative transferability genetic environments sul Escherichia coli isolates collected Penaeus vannamei pork samples three large markets Zhejiang, China. The sulfonamide-resistant E. P. were 90.0% 88.6%, respectively, sul1 was significantly...
ABSTRACT β‐elemene has a variety of anti‐inflammatory, antioxidant, and antitumor effects. Currently, the influence on adrenocortical carcinoma (ACC) malignant progression action mechanism remains unclear. This research aims to explore ACC progression. The impacts cell viability, proliferation, migration, apoptosis were investigated through CCK‐8 assay, clone formation Transwell experiment, Wound healing flow cytometry. miR‐486‐3p expression was analyzed utilizing RT‐qPCR. According...
Vibrio parahaemolyticus is the leading cause of seafood-borne bacterial poisoning in China and a threat to human health worldwide. The aim this study was assess antibiotic resistance profiles distribution heavy metal V. strains isolated Penaeus vannamei from freshwater farms, seawater farms their corresponding markets Zhejiang, relationship between multi drug (MDR) (MHMR). Of 360 P. samples that we tested, 90 (25.00%) were parahaemolyticus-positive, but occurrence pathogenic carrying toxic...
Abstract Focal segmental glomerulosclerosis (FSGS) is a frequent glomerular disease, and the common cause of nephrotic syndrome. However, there no validated diagnostic blood biomarker for FSGS. Here, we performed real-time PCR-based high-throughput miRNA profiling to identify plasma signature We found four miRNAs (miR-17, miR-451, miR-106a, miR-19b) were significantly downregulated in FSGS patients ( n = 97) compared with healthy controls 124) training, validation, blinded-test phases. The...
Obesity is associated with infiltration of macrophages into adipose tissue. However, effects obesity on macrophage and activation in periodontal tissues periodontitis are still to be elucidated.A diet-induced 16-week mouse model was constructed, induced by ligation for 10 days. The consisted (P) control (C) groups, high fat (HF) normal (N) diet conditions. Bone loss (BL) analyzed microcomputed tomography. In tissues, immunohistochemical staining quantitative polymerase chain reaction (qPCR)...
Zn-doped bioactive glass (BGz) micro–nano spheres for dental pulp capping to control infection and inflammation promote tissue regeneration.
Abstract Background and Objective Clinical studies found high levels of hepatocyte growth factor (HGF) expression in patients with periodontitis. Studies suggest that HGF plays an important role periodontitis, is involved inflammation, modulates alveolar bone integrity This study aims to investigate the effects mechanisms progression experimental Methods We used silk thread ligation induce periodontitis HGF‐overexpressing transgenic (HGF‐Tg) wild‐type C57BL/6J mice. The overexpression on...
Abstract The three-dimensional (3D) chromatin structure of Epstein–Barr virus (EBV) within host cells and the underlying mechanisms interaction gene regulation, particularly those involving EBV’s noncoding RNAs (ncRNAs), have remained incompletely characterized. In this study, we employed state-of-the-art techniques 3D genome mapping, including protein-associated analysis with paired-end tag sequencing (ChIA-PET), RNA-associated technique (RDD), super-resolution microscopy, to delineate...
Pseudomonas aeruginosa is noted for its intrinsic antibiotic resistance and capacity of acquiring additional genes. In this study, the genomes nine clinical P. isolates were fully sequenced. An extensive genetic comparison was applied to 18 accessory elements (AGEs; 13 them sequenced in study located within chromosomes) that divided into four groups: five related integrative conjugative (ICEs), mobilizable (IMEs), unit transposons, two IMEs their derivatives. At least 45 genes, involved 10...
The aim of this study is to understand the prevalence and molecular characterization 16S rRNA methylase gene, rmtB, among Escherichia coli strains isolated from bovine mastitis in China. A total 245 E. isolates were collected China between 2013 2014 screened for genes (armA, rmtA, rmtC, rmtD, rmtE, npmA) by polymerase chain reaction. About 5.3% (13/245) carried rmtB gene; highly resistant amikacin. Thirteen rmtB-positive analyzed presence extended-spectrum β-lactamase (bla(TEM), bla(CTX-M),...
Interleukin-1 receptor associated kinase 1 (IRAK1), as a down-stream of toll-like (TLR) signaling, plays important roles in series malignancies. However, the role IRAK1 hepatocellular carcinoma (HCC) remains little known. In our study, reverse transcription-PCR (RT-PCR), Western Blot, and immunohistochemical staining were used to assess mRNA protein levels clinical samples cell lines. Cell counting assay flow cytometry employed analyze effect on cycle apoptosis. Transwell was study...
Abstract Background Fosfomycin has been proven to be a vital choice treat infection caused by multidrug resistance bacteria, especially carbapenem-resistant Klebsiella pneumoniae (CRKP). However, fosfomycin resistant cases reported gradually. In this study, we the fosfomycin-resistant rate in CRKP strains and further revealed molecular mechanisms gene dissemination. Results A total of 294 non-duplicated were collected. And 55 fosfomyin-resistant detected, 94.5% which clustered sequence type...
Lactate accumulation in the tumor microenvironment was shown to be closely related growth and immune escape, suppression of lactate production by inhibiting dehydrogenase A (LDHA) has been pursued as a potential novel antitumor strategy. However, only few potent LDHA inhibitors have developed most them did not show effects vivo. To this end, we designed new obtained inhibitor, ML-05. ML-05 inhibited cellular cell proliferation, which associated with inhibition ATP induction reactive oxygen...
Oral lichen planus (OLP) is a chronic inflammatory oral mucosal disease in which comprehensive inflammation-related cytokines are involved. These commonly produced by immune cells and specific nonimmune including keratinocytes, endothelial fibroblasts. This raises the question of whether fibroblasts OLP lesions contribute to process upon simulation.Primary cultured lichen-planus-associated (OLP AFs, n = 5) normal buccal (NFs, were examined immunohistochemistry, Western blotting reverse...
The carbapenem-resistance genes blaVIM are widely disseminated in Pseudomonas, and frequently harbored within class 1 integrons that reside various mobile genetic elements (MGEs). However, there few reports on detailed dissection of blaVIM-carrying MGEs Pseudomonas.This study presented the complete sequences five blaVIM-2/-4-carrying MGEs, including two plasmids, chromosomal integrative mobilizable (IMEs), one conjugative element (ICE) from different Pseudomonas isolates.The plasmids were...
Chronic hepatitis B virus (HBV) infection is an independent risk factor for the development of hepatocellular carcinoma (HCC). The HBV HBx gene frequently identified as integrant in chromosomal DNA patients with HCC. encodes X protein (HBx), a putative viral oncoprotein that affects transcriptional regulation several cellular genes. Therefore, may be ideal target to impede progression infection-related In this study, integrated was transcriptionally downregulated using artificial...
Abstract Aim To characterize gingival metabolome in high‐fat diet (HFD)‐induced obesity mice with/without periodontitis. Methods HFD‐induced mouse model was established by 16‐week feeding, and a lean control group fed with low‐fat ( n = 21/group). Both models were induced for periodontitis on the left sides molar ligation 10 days, whereas right used as controls. Gingival arginine metabolism analysed non‐targeted/targeted liquid chromatography–mass spectrometry. Results Of 2247 reference...