A5030892053- Influenza Virus Research Studies
- Respiratory viral infections research
- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- Animal Disease Management and Epidemiology
- Liver physiology and pathology
- Fibroblast Growth Factor Research
- Animal Virus Infections Studies
- Circular RNAs in diseases
- Virology and Viral Diseases
- Viral Infectious Diseases and Gene Expression in Insects
- MicroRNA in disease regulation
- Urinary Bladder and Prostate Research
- Cancer-related molecular mechanisms research
- Prostate Cancer Diagnosis and Treatment
- Immune Response and Inflammation
- RNA modifications and cancer
- Prenatal Screening and Diagnostics
- interferon and immune responses
- Genomic variations and chromosomal abnormalities
- Neonatal Respiratory Health Research
- Fetal and Pediatric Neurological Disorders
- Urologic and reproductive health conditions
- RNA Interference and Gene Delivery
- Proteoglycans and glycosaminoglycans research
University of Iowa
2020-2025
Guangxi University of Chinese Medicine
2019-2025
The First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine
2019-2025
Guangzhou Medical University
2014-2024
Guangxi Medical University
2024
Stomatology Hospital
2017-2021
Nanjing Medical University
2019
Jiangsu Academy of Agricultural Sciences
2012-2018
Nanjing Agricultural University
2013-2014
Georgia State University
2014
Oral squamous cell carcinoma (OSCC) is the most frequent oral cancer in world, accounting for more than 90% of all diagnosis. Circular RNAs (circRNAs) are large types non-coding RNAs, demonstrating a great capacity regulating expression genes. However, functions circRNAs still unknown. Recent research revealed that could serve as miRNA-sponge, consequently target genes indirectly, including oncogenes. In this study, we built an apoptotic model with TNF-α, and then confirmed circRNA...
In the current study, we characterized H9N2 influenza viruses isolated from vaccinated flocks in an integrated broiler chicken operation during a 5 year period (1998–2002). Phylogenetic analysis of 8 genes 11 representative showed that they all shared high similarity to first isolate, A/Chicken/Shanghai/F/1998 (Ck/SH/F/98), and clustered same lineages. Furthermore, had 9 nt deletion between positions 206 214 neuraminidase gene. These genetic characteristics strongly suggest these are...
Many novel reassortant influenza viruses of the H9N2 genotype have emerged in aquatic birds southern China since their initial isolation this region 1994. However, genesis and evolution poultry eastern not been investigated systematically. In current study, isolated from during past 10 years were characterized genetically antigenically. Phylogenetic analysis revealed that these undergone extensive reassortment to generate multiple genotypes, including four genotypes (J, F, K, L) never...
Pulmonary ionocytes express high levels of cystic fibrosis transmembrane conductance regulator (CFTR), an anion channel that is critical for hydration the airways and mucociliary clearance. However, cellular mechanisms govern ionocyte specification function remain unclear. We observed increased abundance in (CF) airway epithelium was associated with enhanced expression Sonic Hedgehog (SHH) effectors. In this study, we evaluated whether SHH pathway directly impacts differentiation CFTR...
A number of H5N1 influenza outbreaks have occurred in aquatic birds Asia. As are the natural reservoir viruses and do not usually show clinical disease upon infection, repeated highlighted importance continuous surveillance on birds. In present study we characterized biological properties four avian viruses, which had been isolated from ducks, different animal models. specific pathogen free (SPF) chickens, all isolates were highly pathogenic. SPF mice, S Y moderately However, mallard two low...
Through the detection of circular RNA (circRNA) using expression profiling chips, we searched for circRNAs related to acute myocardial infarction (AMI) and explored their relationship possible mechanisms with AMI.The study subjects included 3 AMI patients controls, circRNA analysis was performed a microarray gene chip identify large differences in between groups construct circRNA-miRNA network.Compared control group, there were 650 differentially expressed found (P < .05, fold change > 2),...
Cystic fibrosis (CF) is caused by mutations in the
To investigate the allelic genotypes of adiponectin (APN) gene polymorphisms (rs1501299) and its association with APN level among Mets patients. A total 410 patients 203 healthy subjects were included in study. The serum levels detected using enzyme-linked immunosorbent assay. G/T at rs1501299 locus restriction fragment length polymorphism polymerase chain reaction technology. significantly lower than control group (15.0 ± 4.9 mg/L vs 27.2 6.5 mg/L, p < 0.05). distribution three was...
Cystic fibrosis (CF) is caused by mutations in the cystic transmembrane conductance regulator (CFTR) gene. While CRISPR-based CFTR editing approaches have shown proof-of-concept for functional rescue primary airway basal cells, induced pluripotent stem and organoid cultures derived from patients with CF, their efficacy remains suboptimal. Here, we developed CuFiCas9(Y66S)eGFP reporter system integrating spCas9 a non-fluorescent Y66S eGFP mutant into CuFi-8 an immortalized human epithelial...
The efficacy of redosing the recombinant adeno-associated virus (rAAV) vector rAAV2.5T to ferret lung is limited by AAV neutralizing antibody (NAb) responses. While immunosuppression strategies have allowed for systemic rAAV repeat dosing, their utility lung-directed gene therapy largely unexplored. To this end, we evaluated two (IS) improve dosing lungs: (1) a combination three IS drugs (Tri-IS) with broad coverage against cellular and humoral responses (methylprednisolone [MP],...
Abstract Background and Objective Clinical studies found high levels of hepatocyte growth factor (HGF) expression in patients with periodontitis. Studies suggest that HGF plays an important role periodontitis, is involved inflammation, modulates alveolar bone integrity This study aims to investigate the effects mechanisms progression experimental Methods We used silk thread ligation induce periodontitis HGF‐overexpressing transgenic (HGF‐Tg) wild‐type C57BL/6J mice. The overexpression on...
Ferroptosis genes have recently been reported to be involved in regulating the development of cancer, but their potential role breast cancer (BRCA) is not fully understood. The purpose this study systematically mechanism ferroptosis BRCA and its relationship with cancer's prognosis, cell infiltration, gene mutation, other clinical features. In study, Cancer Genome Atlas (TCGA-BRCA) database (UCSC Xena) was used mine related patients, prognostic value were screened by Cox regression analysis,...
The H5 subtype highly pathogenic avian influenza (HPAI) virus is one of the greatest threats to global poultry industry. To develop broadly protective subunit vaccine, a recombinant consensus HA sequence (rHA) was constructed and expressed in virus-like particles (rHA VLPs) baculovirus-insect cell system. efficacy rHA VLPs vaccine with or without immunopotentiator (CVCVA5) assessed chickens. Compared commercial Re6 Re6-CVCVA5 vaccines, single dose immunization chickens rHA-CVCVA5 vaccines...
Background To evaluate the application of interferon gamma release assay ( IGRA ), rifampicin resistant real‐time fluorescence quantitative PCR technique Xpert Mycobacterium tuberculosis /rifampicin (Xpert MTB / RIF and levels TNF ‐α TGF ‐β in diagnosis bone joint tuberculosis. Methods Eighty‐six patients with tuberculosis, diagnosed by pathology or microbiology, were examined (T‐ SPOT . TB ) for infection, measured. Results The sensitivity diagnosing was 81.4%; 's 70.9%. combined two...
To investigate the clinical value of noninvasive prenatal testing (NIPT) for fetal chromosomal deletion, duplication, and sex chromosome abnormalities.The study included 6239 pregnant women with singletons in first second trimester pregnancy who received NIPT from December 2017 to June 2019. For at high risk abnormalities indicated by NIPT, amniocentesis was recommended karyotype analysis copy number variation detection verify results analyze abnormalities. Women low no other abnormal...
The dosing interval for effective recombinant adeno-associated virus (rAAV)-mediated gene therapy of cystic fibrosis lung disease remains unknown. Here, we assessed the durability rAAV2.5T-fCFTRΔR-mediated transgene expression and neutralizing antibody (NAb) responses in lungs adult wild-type ferrets. Within first 3 months following rAAV2.5T-fCFTRΔR delivery to lung,
Readministration of recombinant adeno-associated virus (rAAV) may be necessary to treat cystic fibrosis (CF) lung disease using gene therapy. However, little is known about rAAV-mediated immune responses in the lung. Here, we demonstrate suitability ferret for testing AAV2.5T-mediated CFTR delivery and characterization neutralizing-antibody (NAb) responses. AAV2.5T-SP183-hCFTRΔR efficiently transduced both human airway epithelial cultures complemented Cl– currents CF cultures. Delivery...
Breast cancer is the most common female malignancy and major cause of cancer-related death in women. Long non-coding RNAs (lncRNAs), as oncogenic or tumor suppressor factor, involved development progression various cancers. In this study, we sought to investigate function lncRNA CBR3-AS1 breast cancer. We evaluated expression pattern tissues cell lines, explored correlation between survival time patients, probed effect on through loss-of-function gain-of-function strategies. Our results...
Abstract Background: The aim of this study was to investigate the expression tumor necrosis factor alpha (TNF-α) and interleukin-10 (IL-10) in expressed prostatic secretions (EPSs) patients with chronic prostatitis (CP) exosomal protein (PSEP) urine, evaluate its correlation condition. Methods: Urine samples from 310 CP (101 National Institutes Health [NIH] II, 112 NIH IIIa, 97 IIIb, classified according US Health) 110 control group subjects were collected. tested for PSEP by enzyme-linked...