A5039060262- Brucella: diagnosis, epidemiology, treatment
- Monoclonal and Polyclonal Antibodies Research
- Bacillus and Francisella bacterial research
- vaccines and immunoinformatics approaches
- Bacteriophages and microbial interactions
- SARS-CoV-2 and COVID-19 Research
- Immunotherapy and Immune Responses
- Poxvirus research and outbreaks
- Viral Infections and Outbreaks Research
- Viral gastroenteritis research and epidemiology
- Respiratory viral infections research
- Burkholderia infections and melioidosis
- Herpesvirus Infections and Treatments
- Animal Virus Infections Studies
- Virology and Viral Diseases
- Bacterial Genetics and Biotechnology
- Influenza Virus Research Studies
- Virus-based gene therapy research
- Transgenic Plants and Applications
- Viral Infections and Vectors
- Galectins and Cancer Biology
- Prion Diseases and Protein Misfolding
- Electrical and Bioimpedance Tomography
- Invertebrate Immune Response Mechanisms
- RNA and protein synthesis mechanisms
Academy of Military Medical Sciences
2022-2024
National Vaccine and Serum Institute
2015-2023
Beijing Fengtai Hospital
2021-2022
Czech Academy of Sciences, Institute of Biotechnology
2015
Wuxi People's Hospital
2015
Nanjing Medical University
2015
Lassa virus (LASV) belongs to the Arenaviridae family and causes severe hemorrhagic fever in humans. Although many vaccine candidates for exist, no vaccines have been approved clinical use currently. The precursor glycoprotein complex (GPC), which is expressed as a trimer on viral surface, main target development. However, it has significant challenge elicit effective neutralizing antibodies against LASV. In this study, we designed produced prefusion GPC antigen of LASV, named GPCv2. Based...
Abstract The SARS-CoV-2 Omicron variant shows substantial resistance to neutralization by infection- and vaccination-induced antibodies, highlighting the demands for research on continuing discovery of broadly neutralizing antibodies (bnAbs). Here, we developed a panel bnAbs against other variants concern (VOCs) elicited vaccination adenovirus-vectored COVID-19 vaccine (Ad5-nCoV). We also investigated human longitudinal antibody responses following demonstrated how evolved over time. A...
Lassa fever is a hemorrhagic disease caused by virus (LASV), which has been classified the World Health Organization as one of top infectious diseases requiring prioritized research. Previous studies have provided insights into classification and geographic characteristics LASV lineages. However, factor distribution evolution phylodynamics was still limited.
Abstract Brucella spp. are Gram-negative, facultative intracellular bacteria that cause brucellosis in humans and various animals. The threat of has increased, yet currently available live attenuated vaccines still have drawbacks. Therefore, subunit vaccines, produced using protein antigens having the advantage being safe, cost-effective efficacious, urgently needed. In this study, we used core proteome analysis a compositive RV methodology to screen potential broad-spectrum against 213...
The continuous emergence of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) variants highlights the need to update coronavirus 2019 disease (COVID-19) vaccine components. Epitope-based designs targeting conserved and immunorecessive regions SARS-CoV-2 are critically needed. Here, we report an engineered epitope-focused immunogen design based on a novel horseshoe-shaped natural protein scaffold, named ribonuclease inhibitor 1 (RNH1), that can multiply display neutralizing...
The Brucella abortus strain 104M, a spontaneously attenuated strain, has been used as vaccine in humans against brucellosis for 6 decades China. Despite many studies, the molecular mechanisms that cause attenuation are still unclear. Here, we determined whole-genome sequence of 104M and conducted comprehensive comparative analysis whole genome sequences virulent A13334, other reference strains. This revealed highly similar structure between A13334. further genomic A13334 set genes missing...
The genus Henipavirus (HNVs) includes two fatal viruses, namely Nipah virus (NiV) and Hendra (HeV). Since 1994, NiV HeV have been endemic to the Asia–Pacific region responsible for more than 600 cases of infections. Two emerging HNVs, Ghana (GhV) Mojiang (MojV), are speculated be associated with unrecognized human diseases in Africa China, respectively. Despite many efforts develop vaccines against henipaviral diseases, there is presently no licensed vaccine. As HNVs highly pathogenic...
Human brucellosis caused by Brucella is a widespread zoonosis that prevalent in many countries globally. The high homology between members of the genus and Ochrobactrum spp. often complicates determination disease etiology patients. efficient reliable identification distinction are primary interest for both medical surveillance outbreak purposes. A large amount genomic data was analyzed to uncover novel probes containing single-nucleotide polymorphisms (SNPs). GAMOSCE v1.0 software developed...
Brucella spp. are facultative intracellular pathogens that cause chronic brucellosis in humans and animals. The virulence of primarily depends on its successful survival replication host cells. During invasion the tissue, is simultaneously subjected to a variety harsh conditions, including nutrient limitation, low pH, antimicrobial defenses, extreme levels reactive oxygen species (ROS) via immune response. This suggests may be able regulate metabolic adaptation response distinct stresses...
Polysaccharides isolated from natural plants may represent a novel source of vaccine adjuvants. In this research, we focused on plant polysaccharide, PCP-I, which is derived Poria cocos, Chinese traditional herbal medicine. We chose the anthrax protective antigen (PA) as model to evaluate adjuvant ability PCP-I in enhancing immunogenicity and protection PA-based vaccine. According our results, could significantly enhance specific anti-PA antibodies, toxin-neutralizing antibody affinity, well...
Abstract Highly divergent SARS-CoV-2 variants have continuously emerged and spread around the world, updated vaccines innovative vaccination strategies are urgently needed to address global SARS-COV2 pandemic. Here, we established a series of Ad5-vectored variant encoding multiple spike proteins derived from Alpha, Beta, Gamma, Epsilon, Kappa, Delta Omicron lineages analyzed antibody immune responses induced by single-dose prime-boost against emerging concern (VOCs). Single-dose with tended...
ABSTRACT The anthrax protective antigen (PA) is the central component of three-part toxin, and it primary immunogenic in approved AVA vaccine “next-generation” recombinant PA (rPA) vaccines. Animal models have indicated that PA-specific antibodies (AB) are sufficient to protect against infection with Bacillus anthracis . In this study, we investigated domain specificity, affinity, mechanisms neutralization, synergistic effects from a single donor following vaccination rPA vaccine....
Brucella spp. are Gram-negative, facultative intracellular pathogens that cause brucellosis in both humans and animals. The B. abortus vaccine strain 104 M is the only available China for prevention of humans. Although genome has been fully sequenced, current annotations not yet complete. In addition, main mechanisms underpinning its residual toxicity vaccine-induced immune protection have to be elucidated. Mapping proteome will help improve annotation quality, thereby facilitating a greater...
Bacillus anthracis (B. anthracis) is the etiological agent of anthrax affecting both humans and animals. Anthrax toxin (AT) plays a major role in pathogenesis. It includes lethal (LT) edema (ET), which are formed by combination protective antigen (PA) factor (LF) or (EF), respectively. The currently used human vaccine China utilizes live-attenuated B. spores (A16R; pXO1+, pXO2−) that produce but cannot capsule. toxins, especially LT, have key effects on immunogenicity toxicity vaccines....
Summary Adenoviral vectors (AdV) are considered promising candidates for vaccine applications. A prominent group of Toll‐like receptors ( TLR s) participate in the adenovirus‐induced adaptive immune response, yet there is little information regarding role 4 AdV‐induced responses recent literature. We investigated function both and innate to an AdV‐based anthrax vaccine. By immunizing wild‐type knockout 4‐ KO ) mice, we revealed requirement responses. also showed that functions required...
Tularemia is a highly contagious disease caused by infection with Francisella tularensis ( Ft ), pathogenic intracellular gram-negative bacterium that infects wide range of animals and causes severe death in people, making it public health concern. Vaccines are the most effective way to prevent tularemia. However, there no Food Drug Administration (FDA)-approved vaccines thus far due safety concerns. Herein, three membrane proteins , Tul4, OmpA, FopA, molecular chaperone, DnaK, were...
Aluminum (alum) adjuvant is the most extensively used protein subunit vaccine adjuvant, and its effectiveness safety have been widely recognized. The surface charge of antigen determines electrostatic adsorption to alum which directly affects immune efficacy vaccine. In our study, we precisely modified by inserting charged amino acids into flexible region SARS-CoV-2 receptor-binding domain (RBD), achieving a site-specific anchor between immunogen adjuvant. This innovative strategy extended...
Brucellosis is a major threat to public health and animal husbandry. Several in vivo vertebrate models, such as mice, guinea pigs, nonhuman primates, have been used study Brucella pathogenesis, bacteria-host interactions, vaccine efficacy. However, these models limitations whereas the invertebrate Galleria mellonella model cost-effective ethical alternative. The aim of present was examine G. an infection for Brucella. Infection assays were employed validate fitness larval virulence...
Nipah virus (NiV) and Hendra (HeV) are newly emerging dangerous zoonotic pathogens of the Henipavirus genus Paramyxoviridae family. NiV HeV (HNVs) which transmitted by bats cause acute respiratory disease fatal encephalitis in humans. To date, as there is a lack antiviral drugs or effective therapies, development vaccines against those two viruses primary importance, immunogen design crucial to success vaccines. In this study, full-length protein (G), ectodomain (Ge) head domain (Gs)...