A5021486673- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- Diabetes Management and Research
- Cancer-related gene regulation
- Diabetes and associated disorders
- RNA and protein synthesis mechanisms
- Acute Lymphoblastic Leukemia research
- Plant Molecular Biology Research
- Acute Myeloid Leukemia Research
- Intracranial Aneurysms: Treatment and Complications
- Autophagy in Disease and Therapy
- Epigenetics and DNA Methylation
- Chromosomal and Genetic Variations
- Chronic Lymphocytic Leukemia Research
- Diabetes Treatment and Management
- Estrogen and related hormone effects
- Bone and Dental Protein Studies
- Childhood Cancer Survivors' Quality of Life
- Skin Diseases and Diabetes
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Pain Management and Opioid Use
- Hedgehog Signaling Pathway Studies
- HVDC Systems and Fault Protection
- Developmental Biology and Gene Regulation
City of Hope
2020-2025
Beckman Research Institute
2020-2025
Henan University
2024
Sun Yat-sen University
2015-2023
St Bartholomew's Hospital
2022
Loma Linda University
2019
Ministry of Education of the People's Republic of China
2017
Princeton University
2013
More than 100 distinct chemical modifications to RNA have been characterized so far.However, the prevalence, mechanisms and functions of various remain largely unknown.To provide transcriptome-wide landscapes modifications, we developed RMBase v2.0 (http:// rna.sysu.edu.cn/rmbase/), which is a comprehensive database that integrates epitranscriptome sequencing data for exploration post-transcriptional RNAs their relationships with miRNA binding events, disease-related singlenucleotide...
The abnormal transcriptional regulation of non-coding RNAs (ncRNAs) and protein-coding genes (PCGs) is contributed to various biological processes linked with human diseases, but the underlying mechanisms remain elusive. In this study, we developed ChIPBase v2.0 (http://rna.sysu.edu.cn/chipbase/) explore regulatory networks ncRNAs PCGs. has been expanded ∼10 200 curated ChIP-seq datasets, which represent about 20 times expansion when comparing previous released version. We identified...
RNA N 6 -methyladenosine (m A) modification and its regulators fine tune gene expression contribute to tumorigenesis.This study aims uncover the essential role underlying molecular mechanism(s) of m A reader YTHDC1 in promoting triple negative breast cancer (TNBC) metastasis.Methods: In vitro vivo models were employed determine pathological function TNBC metastasis.To identify bona fide target RNAs, we conducted RNA-seq, A-seq, RIP-seq, followed by integrative data analysis validation...
As the most common internal modification of mRNA
Abstract Plant spermatogenesis is a complex process that directly affects crop breeding. A rapid change in gene abundance occurs at early meiosis prophase, when regulation selective. However, how these genes are regulated remains unknown. Here, we show rice reproductive phasiRNAs essential for the elimination of specific set RNAs during meiotic prophase I. These cleave target mRNAs regulatory manner such one phasiRNA can more than gene, and/or single be targeted by to efficiently silence...
Abstract Non-coding RNAs (ncRNAs) are emerging as key regulators of various biological processes. Although thousands ncRNAs have been discovered, the transcriptional mechanisms and networks majority not fully investigated. In this study, we updated ChIPBase to version 3.0 (https://rnasysu.com/chipbase3/) provide most comprehensive regulation atlas protein-coding genes (PCGs). has identified ∼151 187 000 regulatory relationships between ∼171 600 ∼3000 by analyzing ∼55 ChIP-seq datasets, which...
Abstract Up to 80% of the human genome produces “dark matter” RNAs, most which are noncapped RNAs (napRNAs) that frequently act as noncoding (ncRNAs) modulate gene expression. Here, by developing a method, NAP-seq, globally profile full-length sequences napRNAs with various terminal modifications at single-nucleotide resolution, we reveal diverse classes structured ncRNAs. We discover stably expressed linear intron (sliRNAs), class snoRNA-intron (snotrons), embedded in miRNA spacers...
Although thousands of pseudogenes have been annotated in the human genome, their transcriptional regulation, expression profiles and functional mechanisms are largely unknown. In this study, we developed dreamBase (http://rna.sysu.edu.cn/dreamBase) to facilitate investigation DNA modification, RNA regulation protein binding potential expressed from multidimensional high-throughput sequencing data. Based on ∼5500 ChIP-seq DNase-seq datasets, identified genome-wide various...
Polypeptides encoded by long noncoding RNAs (lncRNAs) are a novel class of functional molecules. However, whether these hidden polypeptides participate in the TP53 pathway and play significant biological role is still unclear. Here, we discover that TP53-regulated lncRNAs can encode peptides, two which various human cell lines. Using ribosome profiling RNA-seq approaches HepG2 cells, systematically identified more than 300 further confirmed 15 peptides. Furthermore, several peptides were...
Abstract Aim To determine the effect of osteopontin (OPN) on autophagy and autophagy‐apoptosis interactions after SAH. Methods The endovascular perforation model SAH or sham surgery was performed in a total 86 Sprague‐Dawley male rats. temporal expressions endogenous OPN autophagy‐related proteins (Beclin 1, ATG5, LC3 II to I ratio) were measured rats at different time points (3, 6, 12, 24, 72 hours). Rats randomly divided into three groups: Sham, + Vehicle (PBS, phosphate‐buffered saline),...
Osteopontin (OPN) enhances autophagy, reduces apoptosis, and attenuates early brain injury (EBI) after a subarachnoid hemorrhage (SAH). A total of 87 Sprague–Dawley rats were subjected to sham or SAH operations further investigate the signaling pathway involved in osteopontin-enhanced autophagy during EBI, potential effect recombinant OPN (rOPN) administration improve long-term outcomes SAH. Rats randomly divided into five groups: Sham, + Vehicle (PBS, phosphate-buffered saline), rOPN (5...
Although the overall survival rate of B cell acute lymphoblastic leukemia (B-ALL) in childhood is more than 80%, it merely 30% refractory/relapsed and adult patients with B-ALL. This demonstrates a need for improved therapy targeting this subgroup Here, we show that ten-eleven translocation 1 (TET1) protein, dioxygenase involved DNA demethylation, overexpressed plays crucial oncogenic role independent its catalytic activity Consistent B-ALL, overexpression TET1 alone normal precursor cells...
Rationale: Despite substantial advancement in the treatment of B-cell acute lymphoblastic leukemia (B-ALL), it remains a leading cause cancer mortality children due to high relapse rate. Moreover, long-term survival rates for adult B-ALL patients are still less than 40%. The carrying MLL rearrangements or BCR-ABL fusion represent high-risk subtypes that face particularly dismal prognoses. This study aims identify innovative therapeutic vulnerability B-ALL. Methods: CRISPR-Cas9 screen was...
Current immunotherapies have shown limited success in treating acute myeloid leukemia (AML) due to immune evasion, which often stems from epigenetic dysregulation of immune-related pathways. TET2, a well-studied DNA 5-methylcytosine dioxygenase, is frequently mutated and recognized as tumor suppressor AML. While TET2 deficiency hematopoietic stem/progenitor cells (HSPCs) has been associated with elevated inflammation, also reported promote inflammatory interferon (IFN) signaling solid...
Endogenous small non-coding RNAs (sRNAs), including microRNAs, PIWI-interacting and interfering RNAs, play important gene regulatory roles in animals plants by pairing to the protein-coding transcripts. However, computationally assigning these various sRNAs their target genes remains technically challenging. Recently, a high-throughput degradome sequencing method was applied identify biologically relevant sRNA cleavage sites. In this study, an integrated web-based tool, StarScan (sRNA Scan),...
Although long noncoding RNAs (lncRNAs) have significant tissue specificity, their expression and variability in single cells remain unclear. Here, we developed ColorCells (http://rna.sysu.edu.cn/colorcells/), a resource for comparative analysis of lncRNAs expression, classification functions single-cell RNA-Seq data. was applied to 167 913 publicly available scRNA-Seq datasets from six species, identified batch cell-specific lncRNAs. These show surprising levels between different cell...
The Drosophila embryonic gonad is assembled from two distinct cell types, the Primordial Germ Cells (PGCs) and Somatic Gonadal Precursor cells (SGPs). PGCs form at posterior of blastoderm stage embryos are subsequently carried inside embryo during gastrulation. To reach SGPs, must traverse midgut wall then migrate through mesoderm. A combination local repulsive cues attractive signals emanating SGPs guide migration. We have investigated role hedgehog (hh) pathway gene shifted (shf) in...