A5100441160- Bone Metabolism and Diseases
- Bone health and treatments
- Microtubule and mitosis dynamics
- Plant Molecular Biology Research
- Chromosomal and Genetic Variations
- Cell Adhesion Molecules Research
- Epigenetics and DNA Methylation
- NF-κB Signaling Pathways
- Ubiquitin and proteasome pathways
- Cancer Mechanisms and Therapy
- ATP Synthase and ATPases Research
- Mitochondrial Function and Pathology
- Wnt/β-catenin signaling in development and cancer
- Peptidase Inhibition and Analysis
- Selenium in Biological Systems
- melanin and skin pigmentation
- Skin Protection and Aging
- Thallium and Germanium Studies
- Parathyroid Disorders and Treatments
- Bone health and osteoporosis research
- Histone Deacetylase Inhibitors Research
- Laser Applications in Dentistry and Medicine
- Inflammatory mediators and NSAID effects
- Kruppel-like factors research
- TGF-β signaling in diseases
University of Pennsylvania
2015-2025
Yeungnam University
2010-2024
Yeungnam University College
2007-2024
Gyeongsang National University
2024
Pharmicell (South Korea)
2023
Marine Biodiversity Institute of Korea
2023
Inje University
2017
Kyung Hee University
2016
Sungkyunkwan University
2014-2016
Jungwon University
2015-2016
Bone is maintained by coupled activities of bone-forming osteoblasts/osteocytes and bone-resorbing osteoclasts. Alterations in this relationship can lead to pathologic bone loss such as osteoporosis. It well known that osteogenic cells support osteoclastogenesis via production RANKL. Interestingly, our recently identified marrow mesenchymal cell population—marrow adipogenic lineage precursors (MALPs) form a multidimensional network bone—was computationally demonstrated be the most...
Abstract Osteoclasts are the principal cells that efficiently resorb bone. Numerous studies have attempted to reveal molecular pathways leading differentiation and activation of osteoclasts improve treatment prevention osteoporosis other bone-destructive diseases. While cumulative knowledge osteoclast regulatory molecules, such as receptor activator nuclear factor-kB ligand (RANKL) factor activated T 1 (NFATc1), contributes understanding developmental progression osteoclasts, little is known...
Different stimuli often activate the same intracellular signaling molecules but trigger distinct cell responses. We explored whether or not MAPK induced by macrophage colony-stimulating factor (M-CSF), which is responsible for osteoclast proliferation, differs from that receptor activator of NF-κB ligand (RANKL), essential inducing differentiation. The activation MAPKs M-CSF RANKL differed in terms extent and duration ERK, p38, JNK phosphorylation as well isoform specificity phosphorylation....
Abstract Selenoproteins containing selenium in the form of selenocysteine are critical for bone remodeling. However, their underlying mechanism action is not fully understood. Herein, we report identification selenoprotein W (SELENOW) through large-scale mRNA profiling receptor activator nuclear factor (NF)-κΒ ligand (RANKL)-induced osteoclast differentiation, as a protein that downregulated via RANKL/RANK/tumour necrosis receptor-associated 6/p38 signaling. RNA-sequencing analysis revealed...
Regulation of the formation and function bone-resorbing osteoclasts (OCs) is a key to understanding pathogenesis skeletal disorders. Gene-targeting studies have shown that RANK signaling pathway plays critical role in OC differentiation function. Although pharmaceutical blockade may be viable strategy for preventing bone destruction, implicated multiple biological processes. Recently, cytoplasmic motif was identified specifically involved differentiation. Here, we developed cell-permeable...
Vascular calcification is prevalent in patients with chronic kidney disease and leads to increased cardiovascular morbidity mortality. Although several reports have implicated mitochondrial dysfunction disease, little known about the potential role of process vascular calcification. This study investigated effect α-lipoic acid (ALA), a naturally occurring antioxidant that improves function, on vitro vivo. Calcifying smooth muscle cells (VSMCs) treated inorganic phosphate (Pi) exhibited...
Abstract For cells to exit from pluripotency and commit a lineage, the circuitry of core transcription factor (CTF) network must be extinguished in an orderly manner through epigenetic modifications. However, how this choreographed remodeling at active embryonic stem cell (ESC) genes occurs during differentiation is poorly understood. In study, we demonstrate that C-terminal binding protein 2 (Ctbp2) regulates nucleosome deacetylation (NuRD)-mediated H3K27 facilitates recruitment polycomb...
Systemic transplantation of adipose-derived stem cells (ASCs) is emerging as a novel therapeutic option for functional recovery diverse damaged tissues. This study investigated the effects systemic human ASCs (hASCs) on bone repair. We found that hASCs secrete various cell-activating factors, including hepatocyte growth factor and extracellular matrix proteins. into ovariectomized mice induced an increased number both osteoblasts osteoclasts in tissue thereby prevented loss. also observed...
Excessive bone resorption by osteoclasts (OCs) can result in serious clinical outcomes, including loss that may weaken skeletal or periodontal strength. Proper homeostasis and strength are maintained balancing OC function with the bone-forming of osteoblasts. Unfortunately, current treatments broadly inhibit differentiation also interfere coupled formation. We therefore identified a factor, purinergic receptor P2X5 is highly expressed during maturation phase, which we show here plays no...
Osteoclasts are primary bone-resorbing cells, and receptor-activated NF-kB ligand (RANKL) stimulation is the key driver of osteoclast differentiation. During late-stage differentiation, osteoclasts become multinucleated enlarged (so-called "maturation"), suggesting their need to adapt changing metabolic demands a substantial increase in size. Here, we demonstrate that immunoglobulin superfamily 11 (IgSF11), which required for differentiation through an association with postsynaptic...
Osteoclasts are specialized polyploid cells that resorb bone. Upon stimulation with receptor activator of nuclear factor-κB ligand (RANKL), myeloid precursors commit to becoming polyploid, largely via cell fusion. Polyploidization osteoclasts is necessary for their bone-resorbing activity, but the mechanisms by which polyploidization controlled remain be determined. Here, we demonstrated in addition fusion, incomplete cytokinesis also plays a role osteoclast polyploidization. In vitro...
Here, we show the role of melatonin combined with or without exercise as a determinant multicellular behavior in osteoarthritis. We address relationship between molecular components governing local circadian clock and changes osteoarthritic musculoskeletal axis. Melatonin was injected subcutaneously animals advanced knee osteoarthritis (OA) for 4 weeks. Concurrently, moderate treadmill applied 30 min/day. Morphometric, histological, gene/protein-level analyses were performed cartilage,...
Protocadherin-7 (Pcdh7) is a member of the non-clustered protocadherin δ1 subgroup within cadherin superfamily. Pcdh7 has been shown to control osteoclast differentiation via protein phosphatase 2A (PP2A)-glycogen synthase kinase-3β (GSK3β)-small GTPase signaling axis. As protocadherins serve multiple biological functions, deeper understanding Pcdh7's features valuable. Using an in vitro mouse monocyte cell culture system, we demonstrate that plays role regulating migration by modulating...
Osteoclasts are primary bone resorbing cells. Previously, we described metabolic regulation of osteoclasts through IgSF11-mediated phosphorylation the glycolytic enzyme PKM2. Here, report impact IgSF11-PKM2-mediated on gene expression in osteoclasts, utilizing RNA sequencing engineered to express a chimeric protein, lacking IgSF11, and pharmacologically modulating PKM2 activity. Our analysis identified osteoclast-related genes whose is altered by absence IgSF11 changes This study reveals...
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Abstract Skeletal bone formation and maintenance requires coordinate functions of several cell types, including forming osteoblasts resorbing osteoclasts. Gsα, the stimulatory subunit heterotrimeric G proteins, activates downstream signaling through cAMP plays important roles in skeletal development by regulating osteoblast differentiation. Here, we demonstrate that Gsα also regulates osteoclast differentiation during modeling remodeling. Gnas , gene encoding is imprinted. Mice with paternal...