A5102996041- Glycosylation and Glycoproteins Research
- Ubiquitin and proteasome pathways
- Autoimmune and Inflammatory Disorders Research
- Vasculitis and related conditions
- Carbohydrate Chemistry and Synthesis
- Pancreatic function and diabetes
- Cellular transport and secretion
- Rheumatoid Arthritis Research and Therapies
- Galectins and Cancer Biology
- interferon and immune responses
- Monoclonal and Polyclonal Antibodies Research
- Signaling Pathways in Disease
- RNA and protein synthesis mechanisms
- Kawasaki Disease and Coronary Complications
- Pregnancy and preeclampsia studies
- Inflammatory Myopathies and Dermatomyositis
- Immune Cell Function and Interaction
- Neonatal and Maternal Infections
- Eosinophilic Disorders and Syndromes
- Peptidase Inhibition and Analysis
- Systemic Lupus Erythematosus Research
- Epigenetics and DNA Methylation
- Complement system in diseases
- Cell death mechanisms and regulation
- Immunodeficiency and Autoimmune Disorders
Children's Hospital of Capital Institute of Pediatrics
2013-2025
Maternal and Child Health Hospital of Sichuan Province
2025
Seoul National University
2013-2023
Capital Institute of Pediatrics
2013-2022
Peking University
2021
Seoul National University of Science and Technology
2018-2021
Convergence
2019
New Generation University College
2019
First Affiliated Hospital of Sichuan Medical University
2015
Southwest Medical University
2015
Aberrant glutathione or Ca(2+) homeostasis due to oxidative stress is associated with the pathogenesis of neurodegenerative disorders. The Ca(2+)-permeable transient receptor potential cation (TRPC) channel predominantly expressed in brain, which sensitive stress. However, role TRPC neurodegeneration not known. Here, we report a mechanism TRPC5 activation by oxidants and effect glutathionylated on striatal neurons Huntington's disease. Intracellular oxidized leads via S-glutathionylation at...
Recurrence and drug resistance of breast cancer are still the main reasons for cancer-associated deaths. Cancer stem cell (CSC) model has been proposed as a hypothesis lethality cancer. Molecular mechanisms underlying CSC maintenance unclear. In this study, we generated mammospheres derived from MDA-MB231 cells MCF7 to enrich CSCs performed DNA microarray analysis. We found that expression carboxy terminus HSP70-interacting protein (CHIP) E3 ubiquitin ligase was significantly downregulated...
Glycosylation with O-linked β-N-acetylglucosamine (O-GlcNAc) is one of the protein glycosylations affecting various intracellular events. However, role O-GlcNAcylation in neurodegenerative diseases such as Alzheimer's disease (AD) poorly understood. Mitochondrial adenosine 5′-triphosphate (ATP) synthase a multiprotein complex that synthesizes ATP from ADP and Pi. Here, we found subunit α (ATP5A) was O-GlcNAcylated at Thr432 ATP5A decreased brains AD patients transgenic mouse model, well...
The N-end rule pathway is a proteolytic system in which destabilizing N-terminal residues of short-lived proteins act as degradation determinants (N-degrons). Substrates carrying N-degrons are recognized by N-recognins that mediate ubiquitylation-dependent selective proteolysis through the proteasome. Our previous studies identified mammalian N-recognin family consisting UBR1/E3α, UBR2, UBR4/p600, and UBR5, recognize UBR box. In current study, we addressed physiological function poorly...
Abstract For cells to exit from pluripotency and commit a lineage, the circuitry of core transcription factor (CTF) network must be extinguished in an orderly manner through epigenetic modifications. However, how this choreographed remodeling at active embryonic stem cell (ESC) genes occurs during differentiation is poorly understood. In study, we demonstrate that C-terminal binding protein 2 (Ctbp2) regulates nucleosome deacetylation (NuRD)-mediated H3K27 facilitates recruitment polycomb...
Abstract Nucleocytoplasmic O -GlcNAc transferase (OGT) attaches a single GlcNAc to hydroxyl groups of serine and threonine residues. Although the cellular localisation OGT is important regulate variety processes, molecular mechanisms regulating nuclear unclear. Here, we characterised three amino acids (DFP; residues 451–453) as signal demonstrated that this motif mediated import non-diffusible β-galactosidase. bound importin α5 protein, association was abolished when DFP mutated or deleted....
Significance The Hippo pathway plays a crucial role in maintaining tissue homeostasis. Generally, activated effectors, YAP/TAZ, induce the transcription of their negative regulators, NF2 and LATS2, this feedback loop maintains homeostasis pathway. However, YAP TAZ are consistently hyperactivated various cancer cells, enhancing tumor growth. Our study found that direct-inhibiting kinase YAP/TAZ core component pathway, is modified with O -GlcNAc. LATS2 -GlcNAcylation inhibited its activity by...
Lapatinib, a dual inhibitor of epidermal growth factor receptor (EGFR) and human 2 (HER2) tyrosine kinases, has shown promising results as HER2-positive cancer cells in vitro. However, similar to other EGFR-targeting drugs, acquired resistance lapatinib by remains major clinical challenge. To elucidate mechanisms EGFR/HER2-targeting agents, we performed systematic quantitative comparison the phosphoproteome lapatinib-resistant (LR) gastric (SNU216-LR) versus parental (SNU216) using titanium...
Objective Immune cells from patients with rheumatoid arthritis (RA) express more enolase-1 (ENO1) on their surface than those healthy subjects, and they elicit an enhanced inflammatory response. This study is aimed to identify the ligands of ENO1 that could promote loops in vitro enhance severity vivo. Methods ENO1-binding proteins RA synovial fluid were identified by mass spectromety, affinity was evaluated means a ligand blotting binding assay, plasmon resonance confocal microscopy....
Autoantibodies can facilitate diagnostic and therapeutic means for type 1 diabetes (T1DM). We profiled autoantibodies from serum samples of 16 T1DM patients, 2 diabetic (T2DM) 27 healthy control subjects with normal glucose tolerance (NGT) by using protein microarrays containing 9,480 proteins. Two novel autoantibodies, anti-EEF1A1 anti-UBE2L3, were selected followed immunofluorescence staining pancreas. then tested the validity candidates ELISA in two independent test cohorts: 1) 95 adults...
Identification of predictive biomarkers is essential for the successful development targeted therapy. Insulin-like growth factor 1 receptor (IGF1R) has been examined as a potential therapeutic target various cancers. However, recent clinical trials showed that anti-IGF1R antibody and chemotherapy are not effective treating lung cancer.
Cancer stem cells (CSCs) are a subpopulation of tumor that can self-renew, metastasize, and promote cancer recurrence. A comprehensive characterization the CSC proteome has been hampered due to their scarcity rapid differentiation. Here, we present systematic analysis cell-surface using CSC-like cell line derived from MDA-MB453 breast cells, which exhibited CD44(+) /CD24(-) (where CD is cluster differentiation) phenotype chemoresistance. We identified differentially expressed proteins in...
Indoleamine 2,3-dioxygenases (IDOs) are tryptophan-catabolizing enzymes with immunomodulatory functions. However, the biological role of IDO2 and its relationship IDO1 unknown. To assess between IDO1, we investigated effects co-expression human (h) on hIDO1 activity. Cells co-expressing hIDO2 showed reduced tryptophan metabolic activity compared those expressing only. In a proteomic analysis, hIDO1-expressing cells exhibited enhanced expression proteins related to cell cycle amino acid...
Current serum biomarkers for rheumatoid arthritis (RA) are not highly sensitive or specific to changes of disease activities. Thus, other complementary have been needed improve assessment RA In many diseases, urine has studied as a window provide information measures. Here, we conducted quantitative urinary proteome profiling using liquid chromatography–tandem mass spectrometry (LC–MS/MS) and identified 134 differentially expressed proteins (DEPs) between osteoarthritis (OA) samples. By...
The N-end rule pathway is a proteolytic system in which single N-terminal amino acids of proteins act as class degrons (N-degrons) that determine the half-lives proteins. We have previously identified family mammals N-recognins (termed UBR1, UBR2, UBR4/p600, and UBR5/EDD) whose conserved UBR boxes bind N-degrons to facilitate substrate ubiquitination proteasomal degradation via ubiquitin-proteasome (UPS). Amongst these N-recognins, UBR1 UBR2 mediate proteolysis short-lived regulators...
Distinguishing the luminal subtypes of breast cancer (BC) remaining challenging. Thus, aim this study was to investigate feasibility radiomic signature using intratumoral and peritumoral features obtained from dynamic contrast-enhanced MRI (DCE-MRI) in preoperatively discriminating non-luminal type patients with BC. A total 305 pathologically confirmed BC three hospitals were retrospectively enrolled. The LASSO method then used for selecting features, score (radscore) each patient...
Defective deep placentation, involving abnormal transformation of the spiral arteries in junctional zone myometrium, is known to cause significant obstetric complications, such as preeclampsia (PE), fetal growth restriction, and placental infarction leading death. Serological biomarkers predict diagnose PE would help antenatal care reduce complications. To discover candidate biomarkers, we first performed global proteomic profiling three pairs plasma samples obtained from pregnant women...
Abstract O -linked β- N -acetylglucosamine ( -GlcNAc) is a post-translational modification which occurs on the hydroxyl group of serine or threonine residues nucleocytoplasmic proteins. It has been reported that presence this single sugar motif regulates various biological events by altering fate target proteins, such as their function, localization, and degradation. This study identified SMAD4 novel -GlcNAc-modified protein. component SMAD transcriptional complex, major regulator signaling...
Interleukin (IL)-37 has emerged as a novel anti-inflammatory cytokine that play an immunosuppressive role in regulating inflammatory response. This study aimed to measure IL-37 levels the plasma and peripheral blood mononuclear cells (PBMCs) of patients with systemic juvenile idiopathic arthritis (sJIA), establish correlation between disease activity, laboratory parameters cytokines. The mRNA PBMCs concentrations 46 sJIA 30 age- sex-matched healthy controls were measured by real-time...
Objective. We sought to identify specific microRNA (miRNA) for systemic juvenile idiopathic arthritis (sJIA) and determine the involvement of these miRNA in regulating expression cytokines. Methods. Microarray profiling was performed differentially expressed sJIA plasma. Levels candidate mRNA were assessed by real-time PCR, cytokines measured ELISA. Dual-luciferase reporter assay used validate direct interaction between miR-26a interleukin 6 (IL-6). Results. Forty-eight plasma patients with...
Lupus nephritis (LN) is a major complication of systemic lupus erythematosus (SLE). Conventional biomarkers for assessing renal disease activity are imperfect in predicting clinical outcomes associated with LN. The aim this study to identify urinary protein that reliably reflect the or predict outcomes. A quantitative proteomic analysis was performed biomarker candidates can differentiate between SLE patients and without Selected were further verified by enzyme-linked immunosorbent assay...
Abstract Aim Methylation abnormalities in T lymphocytes have been reported to correlate with systemic lupus erythematosus ( SLE ). Previous studies identified hypomethylation the promoter of several genes linked . Long interspersed nucleotide element‐1 LINE ‐1) constitutes 17–25% human genome, and ‐1 has Limited information is available regarding methylation juvenile JSLE Method levels LINE‐1 peripheral blood mononuclear cells (PBMCs) from 59 47 control samples were examined by...