A5018983636- Osteoarthritis Treatment and Mechanisms
- Knee injuries and reconstruction techniques
- Spine and Intervertebral Disc Pathology
- Electrospun Nanofibers in Biomedical Applications
- Tendon Structure and Treatment
- Periodontal Regeneration and Treatments
- Cellular Mechanics and Interactions
- Silk-based biomaterials and applications
- Musculoskeletal pain and rehabilitation
- Total Knee Arthroplasty Outcomes
- Tissue Engineering and Regenerative Medicine
- Mesenchymal stem cell research
- Bone Tissue Engineering Materials
- Proteoglycans and glycosaminoglycans research
- Shoulder Injury and Treatment
- 3D Printing in Biomedical Research
- Lower Extremity Biomechanics and Pathologies
- Medical Imaging and Analysis
- Orthopaedic implants and arthroplasty
- Bone fractures and treatments
- Hydrogels: synthesis, properties, applications
- Polymer Surface Interaction Studies
- Hippo pathway signaling and YAP/TAZ
- Cell Adhesion Molecules Research
- Orthopedic Surgery and Rehabilitation
University of Pennsylvania
2016-2025
Philadelphia VA Medical Center
2016-2025
California University of Pennsylvania
2011-2024
AO Foundation
2013-2023
Orthopaedic Research
2010-2023
Tokai University
2018-2023
Philadelphia University
2009-2022
Veterans Health Administration
2012-2020
John Wiley & Sons (United States)
2019
Martin University
2019
Due to its avascular nature, articular cartilage exhibits a very limited capacity regenerate and repair. Although much of the tissue-engineered in existence has been successful mimicking morphological biochemical appearance hyaline cartilage, it is generally mechanically inferior natural tissue. In this study, we tested hypothesis that application dynamic deformational loading at physiological strain levels enhances chondrocyte matrix elaboration cell-seeded agarose scaffolds produce more...
Methacrylated hyaluronic acid (HA) hydrogels provide a backbone polymer with which mesenchymal stem cells (MSCs) can interact through several cell surface receptors that are expressed by MSCs, including CD44 and CD168. Previous studies showed this 3D hydrogel environment supports the chondrogenesis of here we demonstrate functional blockade these specific cell–material interactions play role in process. Beyond matrix interactions, cadherin molecules, family transmembrane glycoproteins,...
Mesenchymal stem cells (MSCs) are being recognized as a viable cell source for cartilage repair; however, it still remains challenge to recapitulate the functional properties of native articular using only MSCs. Additionally, MSCs may exhibit hypertrophic phenotype under chondrogenic induction, resulting in calcification after ectopic transplantation. With this mind, objective study was assess whether addition chondrocytes MSC cultures influences tissue-engineered and hypertrophy when...
Significance It is well known that cell proliferation, differentiation, and migration depend strongly on the mechanical stiffness of extracellular matrix (ECM). Natural ECMs also exhibit dissipative (i.e., plastic, viscoelastic) properties, which can modulate cellular behavior. However, to fully utilize this information in bioengineering applications, a systematic understanding role substrate viscosity function needed. Using combined theoretical experimental approaches, we demonstrated...
3D microenvironmental parameters control cell behavior, but can be challenging to investigate over a wide range of conditions. Here, combinatorial hydrogel platform is developed that uses light-mediated thiol-norbornene chemistry encapsulate cells within hydrogels with biochemical gradients made by spatially varied light exposure. Specifically, mesenchymal stem are photoencapsulated in norbornene-modified hyaluronic acid functionalized (0-5 mM) peptides mimic cell-cell or cell-matrix...
Abstract Tissue fibrosis contributes to nearly half of all deaths in the developed world and is characterized by progressive matrix stiffening. Despite this, vitro disease models are mechanically static. Here, we used visible light-mediated stiffening hydrogels investigate cell mechanotransduction a disease-relevant system. Primary hepatic stellate cell-seeded stiffened situ at later time points (following recovery phase post-isolation) displayed accelerated signaling kinetics both early...
Mesenchymal stem cell (MSC) differentiation is mediated by soluble and physical cues. In this study, we investigated differentiation-induced transformations in MSC cellular nuclear biophysical properties queried their role mechanosensation. Our data show that nuclei differentiated bovine human MSCs stiffen become resistant to deformation. This attenuated deformation was governed restructuring of Lamin A/C increased heterochromatin content. change stiffness sensitized...
EGFR activation by overexpressing HBEGF in cartilage or intra-articular delivery of TGFα nanoparticles attenuates osteoarthritis development mice.
Osteoarthritis (OA) affects multiple tissues in the knee joint, including synovium and intra-articular adipose tissue (IAAT) that are attached to each other. However, whether these two share same progenitor cells hence function as a single unit joint homeostasis diseases is largely unknown. Single-cell transcriptomic profiling of infrapatellar fat pad (IFP), largest IAAT, from control OA mice revealed five mesenchymal clusters predicted (MPCs) common progenitors for other cells: synovial...
It has previously been demonstrated that dynamic deformational loading of chondrocyte-seeded agarose hydrogels over the course 1 month can increase construct mechanical and biochemical properties relative to free-swelling controls. The present study examines manner in which two mediators matrix biosynthesis, growth factors TGF-β1 IGF-I, interact with applied loading. Under conditions control medium (C), [proteoglycan content][collagen content][equilibrium aggregate modulus] cell-laden (10 ×...