A5100609547- Cellular Mechanics and Interactions
- 3D Printing in Biomedical Research
- Tissue Engineering and Regenerative Medicine
- Cell Adhesion Molecules Research
- Microfluidic and Bio-sensing Technologies
- Electrospun Nanofibers in Biomedical Applications
- Bone Tissue Engineering Materials
- Cancer Cells and Metastasis
- Force Microscopy Techniques and Applications
- Angiogenesis and VEGF in Cancer
- Solar and Space Plasma Dynamics
- Neuroscience and Neural Engineering
- Cardiomyopathy and Myosin Studies
- Pluripotent Stem Cells Research
- Nanofabrication and Lithography Techniques
- Ionosphere and magnetosphere dynamics
- Tendon Structure and Treatment
- Peptidase Inhibition and Analysis
- Liver physiology and pathology
- Microtubule and mitosis dynamics
- Hippo pathway signaling and YAP/TAZ
- Polymer Surface Interaction Studies
- CAR-T cell therapy research
- RNA Research and Splicing
- Spaceflight effects on biology
Harvard University
2016-2025
Boston University
2016-2025
Micron Corporation (United States)
2025
Johns Hopkins University
2003-2024
Stanford University
2007-2024
University of California, San Francisco
2022-2024
Cancer Research Center
2023-2024
University of California, Los Angeles
2007-2024
Brigham and Women's Hospital
1995-2024
National University of Singapore
2012-2024
Human and bovine capillary endothelial cells were switched from growth to apoptosis by using micropatterned substrates that contained extracellular matrix-coated adhesive islands of decreasing size progressively restrict cell extension. Cell spreading also was varied while maintaining the total cell-matrix contact area constant changing spacing between multiple focal adhesion-sized islands. shape found govern whether individual grow or die, regardless type matrix protein antibody integrin...
We describe an approach to manipulate and measure mechanical interactions between cells their underlying substrates by using microfabricated arrays of elastomeric, microneedle-like posts. By controlling the geometry posts, we varied compliance substrate while holding other surface properties constant. Cells attached to, spread across, deflected multiple The deflections posts occurred independently neighboring and, therefore, directly reported subcellular distribution traction forces. report...
We report here that living cells and nuclei are hard-wired such a mechanical tug on cell surface receptors can immediately change the organization of molecular assemblies in cytoplasm nucleus. When integrins were pulled by micromanipulating bound microbeads or micropipettes, cytoskeletal filaments reoriented, distorted, nucleoli redistributed along axis applied tension field. These effects specific for integrins, independent cortical membrane distortion, mediated direct linkages between...
Spatial patterns of cellular growth generate mechanical stresses that help to push, fold, expand, and deform tissues into their specific forms. Genetic factors are thought specify other behaviors drive morphogenesis. Here, we show tissue form itself can feed back regulate proliferation. Using micro-fabrication control the organization sheets cells, demonstrated emergence stable proliferative foci. Regions concentrated corresponded regions high tractional stress generated within sheet, as...
The control of cell position and function is a fundamental focus in the development applications ranging from cellular biosensors to tissue engineering. Using microcontact printing self-assembled monolayers (SAMs) alkanethiolates on gold, we manufactured substrates that contained micrometer-scale islands extracellular matrix (ECM) separated by nonadhesive regions such pattern determined distribution bovine human endothelial cells. In addition, size geometry were shown shape. Traditional...
Actomyosin contractility affects cellular organization within tissues in part through the generation of mechanical forces at sites cell–matrix and cell–cell contact. While increased loading adhesions results focal adhesion growth, whether drive changes size remains an open question. To investigate responsiveness adherens junctions (AJ) to force, we adapted a system microfabricated force sensors quantitatively report tugging AJ size. We observed that was modulated by endothelial forces: AJs...
There is increasing demand for automated and quantitative cell culture technology, driven both by the intense activity in stem biology emergence of systems biology. We built a fully screening system based on microfluidic chip that creates arbitrary media formulations 96 independent chambers maintains viability weeks. Individual conditions are customized terms seeding density, composition medium, feeding schedule, each chamber imaged with time-lapse microscopy. Using this device, we perform...
Human mutations that truncate the massive sarcomere protein titin [TTN-truncating variants (TTNtvs)] are most common genetic cause for dilated cardiomyopathy (DCM), a major of heart failure and premature death. Here we show cardiac microtissues engineered from human induced pluripotent stem (iPS) cells powerful system evaluating pathogenicity gene variants. We found certain missense mutations, like TTNtvs, diminish contractile performance pathogenic. By combining functional analyses with RNA...