A5020651722- Cellular Mechanics and Interactions
- 3D Printing in Biomedical Research
- Barrier Structure and Function Studies
- Proteoglycans and glycosaminoglycans research
- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- Cancer, Hypoxia, and Metabolism
- Blood properties and coagulation
- Angiogenesis and VEGF in Cancer
- Tendon Structure and Treatment
- Skin and Cellular Biology Research
- Long-Term Effects of COVID-19
- Nerve injury and regeneration
- Immune cells in cancer
- Cell Adhesion Molecules Research
- Cardiac Fibrosis and Remodeling
- Hydrogels: synthesis, properties, applications
- Neuroinflammation and Neurodegeneration Mechanisms
- Cardiovascular Health and Disease Prevention
- Mesenchymal stem cell research
- nanoparticles nucleation surface interactions
- Computational Drug Discovery Methods
- Silk-based biomaterials and applications
- Advanced Thermodynamics and Statistical Mechanics
- Vagus Nerve Stimulation Research
Rowan University
2016-2025
California University of Pennsylvania
2025
University of Pennsylvania
2012-2019
Philadelphia University
2015
University of Michigan
2010-2014
Bioengineering Center
2014
Rensselaer Polytechnic Institute
2009
Princeton University
2007
Solid organs transport fluids through distinct vascular networks that are biophysically and biochemically entangled, creating complex three-dimensional (3D) regimes have remained difficult to produce study. We establish intravascular multivascular design freedoms with photopolymerizable hydrogels by using food dye additives as biocompatible yet potent photoabsorbers for projection stereolithography. demonstrate monolithic transparent hydrogels, produced in minutes, comprising efficient 3D...
Angiogenesis is a complex morphogenetic process whereby endothelial cells from existing vessels invade as multicellular sprouts to form new vessels. Here, we have engineered unique organotypic model of angiogenic sprouting and neovessel formation that originates preformed artificial fully encapsulated within 3D extracellular matrix. Using this model, screened the effects factors identified two distinct cocktails promoted robust sprouting. The in our system exhibited hallmark structural...
As researchers across the globe have focused their attention on understanding SARS-CoV-2, picture that is emerging of a virus has serious effects vasculature in multiple organ systems including cerebral vasculature. Observed central nervous system include neurological symptoms (headache, nausea, dizziness), fatal microclot formation and rare cases encephalitis. However, our how causes these mild to severe impacted remains unclear. Thus, results presented this report explored whether...
The density and architecture of capillary beds that form within a tissue depend on many factors, including local metabolic demand blood flow. Here, using microfluidic control fluid mechanics, we show the existence previously unappreciated flow-induced shear stress threshold triggers angiogenic sprouting. Both intraluminal over endothelium transmural flow through above 10 dyn/cm(2) triggered endothelial cells to sprout invade into underlying matrix, this is not impacted by maturation...
// Keri L. Schadler 1 , Nicholas J. Thomas Peter A. Galie 2 Dong Ha Bhang Kerry C. Roby Prince Addai Jacob E. Till Kathleen Sturgeon Alexander Zaslavsky Christopher S. Chen 3 Sandra Ryeom Department of Cancer Biology, Perelman School Medicine, University Pennsylvania, Abramson Family Research Institute, Philadelphia, PA 19104, USA Physiology, Bioengineering, Boston University, Boston, MA 02215, Correspondence to: Ryeom, email: sryeom@upenn.edu Keywords: tumor vascular normalization, NFAT,...
Gels formed by semiflexible filaments such as most biopolymers exhibit non-linear behavior in their response to shear deformation, e.g., with a pronounced strain stiffening and negative normal stress. These stresses suggest that networks would collapse axially when subject This coupling of axial deformations can have particularly important consequences for extracellular matrices collagenous tissues. Although measurements uniaxial moduli been made on biopolymer gels, these not directly...
Abstract The migration of cells through constricting spaces or along fibrous tracks in tissues is important for many biological processes and depends on the mechanical properties a cytoskeleton made up three different filaments: F‐actin, microtubules, intermediate filaments. signaling pathways cytoskeletal structures that control cell motility 2D are often very from those 3D. Previous studies have shown filaments can promote actin‐driven protrusions at edge, but little effect overall flat...
We present theoretical and experimental studies of the elastic response fibrous networks subjected to uniaxial strain. Uniaxial compression or extension is applied extracellular fibrin collagen using a shear rheometer with free water in/outflow. Both stress network modulus are measured. Prior work [van Oosten, et al., Sci. Rep., 2015, 6, 19270] has shown softening/stiffening these under compression/extension, together nonlinear shear, but origin such behaviour remains poorly understood....
ABSTRACT As researchers across the globe have focused their attention on understanding SARS-CoV-2, picture that is emerging of a virus has serious effects vasculature in multiple organ systems including cerebral vasculature. Observed central nervous system includes neurological symptoms (headache, nausea, dizziness), fatal microclot formation and rare cases encephalitis. However, our how causes these mild to severe impacted remains unclear. Thus, results presented this report explored...
A device able to apply multiple levels of shear stress cells plated on polyacrylamide gels physiological stiffness.
Injectable hydrogels for cell delivery and tissue regeneration have several advantages over pre-fabricated scaffolds that require more invasive transplantation procedures, but lack the ability to implement tunable topologies. Here, we describe an approach create patternable injectable using magnetically-responsive (MR) self-assembling peptide hydrogels, validate their efficacy promote align axon infiltration at site of a spinal cord injury. In vitro experiments reveal parameters needed...
Fluid shear stress is an important mediator of vascular permeability, yet the molecular mechanisms underlying effect on blood-brain barrier (BBB) have to be clarified in cerebral vasculature despite its importance for brain homeostasis. The goal this study probe components mechanotransduction within BBB gain a better understanding pathologies associated with changes perfusion including ischemic stroke. Interrogating effects vivo complicated by complexity factors parenchyma and difficulty...
Alterations of the extracellular matrix (ECM), including both mechanical (such as stiffening ECM) and chemical variation adhesion proteins deposition hyaluronic acid (HA)) changes, in malignant tissues have been shown to mediate tumor progression. To survey how cells from different tissue types respond various changes ECM mechanics composition, we measured physical characteristics (adherent area, shape, cell stiffness, speed) 25 cancer 5 non-tumorigenic lines on 7 substrate conditions. Our...
Abstract The flow inside the perivascular space (PVS) is modeled using a first-principles approach in order to investigate how cerebrospinal fluid (CSF) enters brain through permeable layer of glial cells. Lubrication theory employed deal with thin annular gap between an impermeable artery and tissue. has imposed peristaltic deformation deformable tissue by means elastic Hooke’s law. model solved numerically, discovering that wave induces steady streaming to/from which strongly depends on...