Injectable hydrogels are becoming increasingly important in the fields of tissue engineering and drug delivery due to their tunable properties, controllable degradation, high water content, ability deliver them a minimally invasive manner. Shear-thinning is one promising technique for application injectable hydrogels, where preformed can be injected by shear stress (during injection) quickly self-heal after removal shear. Importantly, these gels used biological molecules cells during...

Three-dimensional (3D) printing is becoming an increasingly common technique to fabricate scaffolds and devices for tissue engineering applications. This due the potential of 3D provide patient-specific designs, high structural complexity, rapid on-demand fabrication at a low-cost. One major bottlenecks that limits widespread acceptance in biomanufacturing lack diversity “biomaterial inks”. Printability biomaterial determined by technique. Although wide range inks including polymers,...

Methacrylated hyaluronic acid (HA) hydrogels provide a backbone polymer with which mesenchymal stem cells (MSCs) can interact through several cell surface receptors that are expressed by MSCs, including CD44 and CD168. Previous studies showed this 3D hydrogel environment supports the chondrogenesis of here we demonstrate functional blockade these specific cell–material interactions play role in process. Beyond matrix interactions, cadherin molecules, family transmembrane glycoproteins,...

Abstract Hydrogels with controlled surface patterns are useful for a range of applications, including in microdevices, sensors, coatings, and adhesives. In this work, simple robust method to generate wide osmotically driven patterns, random, lamellar, peanut, hexagonal structures is developed. This does not require the use organic solvents swelling, pre‐patterning film surface, or coating second layer on gel. The fabricated by exposing photocurable formulation light while open air then using...

We investigated solvent induced transition of surface instability from wrinkles to creases in poly(2-hydroxyethyl methacrylate) (PHEMA) gels with depth-wise crosslinking gradients. The mode and morphology patterns was found be dependent on the equilibrium linear expansion, which a function crosslinker concentration solvent–polymer interaction. maximum expansion obtained when PHEMA film swollen good solvent, had Hildebrand solubility parameter (δs) close that gels, 26.6 29.6 MPa1/2. In...

The interlayer bonding strength of 3D-printed PEEK specimens is studied using a three-point flexural test combined with design experiment (DOE) approach to reveal correlations between printing parameters and the end-use properties.

Marine mussels anchor to a variety of surfaces by secreting liquid proteins that harden and form water-resistant bonds surfaces. Studies have revealed these mussel adhesive contain an unusual amino acid, 3,4-dihydroxy-L-phenylalanine (DOPA), which is believed be responsible for the cohesive properties proteins. To separate roles DOPA, we incorporated DOPA into midblock poly(methyl methacrylate)−poly(methacrylic acid)−poly(methyl methacrylate) (PMMA−PMAA−PMMA) triblock copolymers....

Hydrogels undergo extensive three-dimensional volume changes when immersed in water, the degree of which is determined by network chemical composition and crosslinking. When hydrogel attached to a rigid substrate, it swells preferentially perpendicular substrate. This anisotropic swelling generates compressive stress, drives formation surface patterns exceeding critical stress value (σ ≥ σc). In order develop an indepth understanding mechanism pattern hydrogels, we investigated dynamic...

The dynamic alignment of cells and matrix is critical in many biological processes, including during tissue development the progression a variety diseases; yet, nearly all vitro models are static. Thus, it great interest to temporally spatially manipulate cellular better understand develop strategies control these processes. Here, strain-responsive buckling patterns on PDMS substrates used dynamically human mesenchymal stem cell (hMSC) organization. results indicate that pattern recognition...

The adhesive proteins secreted by marine mussels form a natural glue that cures rapidly to strong and durable bonds in aqueous environments. These mussel contain an unusual amino acid, 3,4-dihydroxy-L-phenylalanine (DOPA), which is largely responsible for their cohesive strengths. In this study, we incorporated DOPA into diblock triblock polymers developed membrane contact experiment assess the interactions of these materials with TiO2 tissue surfaces. typical micrometer-thick...

Micro-scale topography mimics stem cell patterning in human interfollicular epidermal cells.

Age-related intimal stiffening is associated with increased endothelium permeability, an initiating step in atherosclerosis. Notably, addition to a bulk increase matrix stiffness, the aged intima also exhibits spatial stiffness heterogeneity. We investigate effect of heterogeneous on endothelial cells. Methacrylated hyaluronic acid hydrogels are fabricated and photopatterned create substrates 50-and 100 μm squares containing soft stiff regions 2.7 ± 0.7 10.3 3.9 kPa. On patterned matrices,...