A5074132361- Cellular Mechanics and Interactions
- 3D Printing in Biomedical Research
- Electrospun Nanofibers in Biomedical Applications
- Angiogenesis and VEGF in Cancer
- Cancer Cells and Metastasis
- Cardiovascular Function and Risk Factors
- Tissue Engineering and Regenerative Medicine
- Pulmonary Hypertension Research and Treatments
- Photoacoustic and Ultrasonic Imaging
- Connective tissue disorders research
- Cell Adhesion Molecules Research
- Tendon Structure and Treatment
- Cardiac Imaging and Diagnostics
- Acute Myocardial Infarction Research
- Blood properties and coagulation
- Advanced Fluorescence Microscopy Techniques
- Advanced Image Fusion Techniques
- Image Enhancement Techniques
- Thermoregulation and physiological responses
- Mesenchymal stem cell research
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Extracellular vesicles in disease
- Inhalation and Respiratory Drug Delivery
- Electrohydrodynamics and Fluid Dynamics
- Advanced Image Processing Techniques
University of Michigan–Ann Arbor
2018-2023
Ann Arbor Center for Independent Living
2020-2021
Boston University
2015
Princess Alexandra Hospital
2014
Princess Alexandra Hospital
2014
The University of Queensland
2014
Institute of Cardiology
2014
Cells select from a diverse repertoire of migration strategies. Recent developments in tunable biomaterials have helped identify how extracellular matrix properties influence migration, however, many settings lack the fibrous architecture characteristic native tissues. To investigate contexts, we independently varied alignment and stiffness synthetic 3D fiber matrices identified two phenotypically distinct modes. In contrast to stiff where cells migrated continuously traditional mesenchymal...
Abstract Vasculogenesis is the de novo formation of a vascular network from individual endothelial progenitor cells occurring during embryonic development, organogenesis, and adult neovascularization. can be mimicked studied in vitro using assays, which (ECs) spontaneously form capillary-like structures when seeded appropriate microenvironment. While biochemical regulators have been well these role mechanical topographical properties extracellular matrix (ECM) less understood. Here, we...
Physical features of the extracellular matrix (ECM) heavily influence cell migration strategies and efficiency. Migration in on fibrous ECMs is significant physiologic importance, but limitations ability to experimentally define diameter, density, alignment native vitro have hampered our understanding how these properties affect this basic function. Here, we designed a high-throughput platform that models ECM as collections lines cell-adhesive fibronectin flat surface eliminate effects...
Cellular phenotype is heavily influenced by the extracellular matrix (ECM), a complex and tissue-specific three-dimensional structure with distinct biophysical biochemical properties. As naturally derived cell culture platforms are difficult to controllably modulate, engineered synthetic ECMs have facilitated our understanding of how specific properties direct behavior. However, approaches typically lack fibrous topography, hallmark stromal interstitial in vivo. To construct tunable...
The formation of functional microvasculature results from physical and soluble microenvironmental cues that balance endothelial cell migration with proliferation during multicellular sprouting morphogenesis.
Acoustically-responsive scaffolds (ARSs), which are fibrin hydrogels containing monodispersed perfluorocarbon (PFC) emulsions, respond to ultrasound in an on-demand, spatiotemporally-controlled manner via a mechanism termed acoustic droplet vaporization (ADV). Previously, ADV has been used control the release of bioactive payloads from ARSs stimulate regenerative processes. In this study, we classical nucleation theory (CNT) predict pressure emulsions different PFC cores as well...
Supportive stromal cells of mesenchymal origins regulate vascular morphogenesis in developmental, pathological, and regenerative contexts, contributing to vessel formation, maturation, long-term stability, part via the secretion bioactive molecules. In this work, we adapted a microfluidic lab-on-a-chip system that enables formation perfusion microvascular capillary beds with connections arteriole-scale endothelialized channels explore how cell (SC) identity influences endothelial (EC)...
Angiogenesis is a complex, multicellular process that involves bidirectional interactions between extracellular matrix (ECM) and collectively invading endothelial cell (EC) sprouts extend the microvasculature during development, wound healing, disease processes. While many aspects of angiogenesis have been well studied, relationship sprout morphology subsequent neovessel function remains relatively unknown. Here, we investigated how various soluble physical cues regulate sprouting speed...
Synthetic hydrogels represent an exciting avenue in the field of regenerative biomaterials given their injectability, orthogonally tunable mechanical properties, and potential for modular inclusion cellular cues. Separately, recent advances soluble factor release technology have facilitated control over milieu cell microenvironments via microparticles. A composite hydrogel incorporating both these components can robustly mediate tendon healing following a single injection. Here, synthetic...
Abstract Intercellular communication is critical to the formation and homeostatic function of all tissues. Previous work has shown that cells can communicate mechanically via transmission cell‐generated forces through their surrounding extracellular matrix, but this process not well understood. Here, defined, synthetic electrospun fibrous matrices are utilized in conjunction with a microfabrication‐based cell patterning approach examine mechanical intercellular (MIC) between endothelial...
Acoustic droplet vaporization (ADV) of phase shift emulsion (green) generates different bubble morphologies, and spatiotemporally tunes the micromechanics fibrin gels (red).
Abstract Capillary scale vascularization is critical to the survival of engineered 3D tissues and remains an outstanding challenge for field tissue engineering. Current methods generate micro‐scale vasculatures such as printing, two photon hydrogel ablation, angiogenesis, vasculogenic assembly face challenges in rapidly creating organized, highly vascularized at capillary length‐scales. Within metabolically demanding tissues, native beds are organized densely packed achieve adequate delivery...
Abstract Mechanical interactions between cells and their surrounding extracellular matrix (ECM) guide many fundamental cell behaviors. Native connective tissue consists of highly organized, 3D networks ECM fibers with complex, nonlinear mechanical properties. The most abundant stromal component is fibrillar type I collagen, which often possesses a wavy, crimped morphology that confers strain- load-dependent behavior. Here, we established new simple method for engineering electrospun fibrous...
Emerging cell-based therapies such as stem cell therapy and immunotherapy have attracted broad attention in both biological research clinical practice. However, a long-standing technical gap of is the difficulty directly assessing treatment efficacy via tracking therapeutically administered cells. Therefore, imaging techniques to follow
The functional membrane proteins on tumor-cell-derived EVs contain a large amount of biomolecular information, and can serve as comprehensive marker to delineate the molecular nature cancer. However, due low secretion rates, it is difficult perform accurate quantification analysis with conventional EV technologies such Western blots. Here, we introduce multifunctional technology based an automated microfluidic chemiluminescent ELISA (Enzyme-Linked ImmunoSorbent Assay) platform. With this...
The extracellular matrix (ECM) influences biological processes associated with tissue development and disease progression. However, robust cell‐free techniques to control fiber alignment of naturally derived ECM proteins, such as fibronectin (Fn), remain elusive. It is demonstrated that controlled hydrodynamics Fn solutions at the air/fluid interface porous tessellated polymer scaffolds (TPSs) generates suspended 3D fibrillar networks across multiple length scales (<1, 1–20 μm, extended...
ABSTRACT Intercellular communication is critical to the development and homeostatic function of all tissues. Previous work has shown that cells can communicate mechanically via transmission cell-generated forces through their surrounding extracellular matrix, but this process not well understood. Here, we utilized synthetic, electrospun fibrous matrices in conjunction with a microfabrication-based cell patterning approach examine mechanical intercellular (MIC) between endothelial (ECs)...
ABSTRACT The peritumoral stroma is a complex 3D tissue that provides cells with myriad biophysical and biochemical cues. Histologic observations suggest during metastatic spread of carcinomas, these cues influence transformed epithelial cells, prompting diversity migration modes spanning single cell multicellular phenotypes. Purported consequences variations in tumor escape strategies include differential capability therapy resistance. Therefore, understanding how from the stromal...