A5042176884- Reproductive Biology and Fertility
- Ovarian function and disorders
- Tissue Engineering and Regenerative Medicine
- Sperm and Testicular Function
- Cellular Mechanics and Interactions
- 3D Printing in Biomedical Research
- Reproductive System and Pregnancy
- Pluripotent Stem Cells Research
- Electrospun Nanofibers in Biomedical Applications
- Hydrogels: synthesis, properties, applications
- Renal and related cancers
- biodegradable polymer synthesis and properties
- LGBTQ Health, Identity, and Policy
- Single-cell and spatial transcriptomics
- Nanoparticle-Based Drug Delivery
- Tendon Structure and Treatment
- Reproductive Health and Technologies
- Cancer Cells and Metastasis
- Hormonal and reproductive studies
- Sex and Gender in Healthcare
- Cancer Treatment and Pharmacology
- Advanced Drug Delivery Systems
- RNA Interference and Gene Delivery
- Advanced Polymer Synthesis and Characterization
- Angiogenesis and VEGF in Cancer
University of Michigan
2016-2025
Michigan Medicine
2023-2025
Michigan United
2017-2024
Ann Arbor Center for Independent Living
2018
Michigan Center for Translational Pathology
2018
Weatherford College
2014
Hebrew University of Jerusalem
2002-2013
Northwestern University
2009-2012
Hadassah Medical Center
2006
The reproductive and endocrine functions of the ovary involve spatially defined interactions among specialized cell populations. Despite ovary’s importance in fertility health, functional attributes ovarian cells are largely uncharacterized. Here, we profiled >18,000 genes 257 regions from ovaries two premenopausal donors to examine units ovary. We also generated single-cell RNA sequencing data for 21,198 three additional identified four major types immune subtypes. Custom selection...
Ovarian cryopreservation before chemotherapy and autotransplantation post-treatment can restore fertility to women with premature ovarian failure. Although the majority of primordial follicles survive cycle, follicular pool is reduced after transplantation due ischemic death. Therefore, we engineered a biomaterial-based system promote angiogenesis in mouse model transplantation. To mimic clinical situation sterility, bilateral ovariectomy was performed 2 weeks transplantation, during which...
Tunable properties of multi-arm poly(ethylene glycol) (PEG) hydrogel, crosslinked by Michael-type addition, support diverse applications in tissue engineering. Bioactive modification PEG is achieved incorporating integrin binding sequences, like RGD, and crosslinking with tri-functional protease sensitive peptide (GCYKNRGCYKNRCG), which compete for the same reactive groups PEG. This competition leads to a narrow range conditions that sufficient density provide structural control. Kinetics...
Female cancer patients who seek fertility preservation but cannot undergo ovarian stimulation and embryo may consider 1) retrieval of immature oocytes followed by in vitro maturation (IVM) or 2) tissue cryopreservation transplantation follicle culture. Conventional IVM is carried out during the follicular phase menstrual cycle. There limited evidence demonstrating that oocyte retrieved luteal can mature be fertilized to produce viable embryos. While culture successful rodents, its...
Cellular phenotype is heavily influenced by the extracellular matrix (ECM), a complex and tissue-specific three-dimensional structure with distinct biophysical biochemical properties. As naturally derived cell culture platforms are difficult to controllably modulate, engineered synthetic ECMs have facilitated our understanding of how specific properties direct behavior. However, approaches typically lack fibrous topography, hallmark stromal interstitial in vivo. To construct tunable...
Many prepubertal girls and young women suffer from premature ovarian insufficiency induced by chemotherapy given for treatment of cancer autoimmune diseases. Auto-transplantation cryopreserved tissue could restore the lost endocrine function fertility. Unfortunately, ischemia, inconsistent graft quality risk re-introducing malignant cells may stand in way clinical translation this approach. To address these risks limitations, we engineered an artificial ovary immature follicles using a...
Ovarian tissue cryopreservation is gaining importance as a successful method to restore fertility girls and young women at high risk of sterility. However, there are concerns regarding the safety transplantation after ovarian due reintroducing cancer cells causing disease recurrence. In these cases, development culture systems that support oocyte from primordial follicle stage required. Notable achievements have been reached in human vitro growth past decade. Currently, for based on...
Abstract New degradable poly(ester anhydride)s were prepared by the melt polycondensation of diacid oligomers poly(sebacic acid) (PSA) transesterified with ricinoleic acid. The transesterification PSA acid to form was conducted via a bulk reaction between high molecular weight and A systematic study on synthesis, characterization, degradation in vitro , drug release, stability these polymers performed. Polymers weight‐average weights 2000–60,000 melting temperatures 24–77 °C obtained for...
The ovarian follicle is the functional unit of ovary that secretes sex hormones and supports oocyte maturation. In vitro techniques provide a tool to model development in order investigate basic biology, are further being developed as technique preserve fertility clinic1-4. Our culture system employs hydrogels mimic native environment by maintaining 3D follicular architecture, cell-cell interactions paracrine signaling direct 5. Previously, follicles were successfully cultured alginate, an...
The cryopreservation and autotransplantation of ovarian tissue is emerging as a powerful approach for preserving fertility. However, cancer patients, it may not be possible to transplant due the risk re-seeding disease. We investigated strategies transplantation individually isolated follicles minimize re-introducing cells present within vasculature stroma. Procedures large-scale isolation early-stage their encapsulation into fibrin hydrogels were developed. For in vivo validation studies,...
Cancer survivorship rates have drastically increased due to improved efficacy of oncologic treatments. Consequently, clinical concerns shifted from solely focusing on survival quality life, with fertility preservation as an important consideration. Among strategies for female patients, ovarian tissue cryopreservation and subsequent reimplantation has been the only option available cancer survivors cryopreserved tissue. However, follicle atresia after transplantation risk reintroducing...
Premature ovarian insufficiency is a common complication of anticancer treatments in young women and girls. The ovary complex, highly regulated reproductive organ, whose proper function contingent upon the bidirectional endocrine, paracrine, autocrine signaling. These factors facilitate development follicles, functional units ovary, to progress from gonadotropin-independent, paracrine-controlled early stage gonadotropin-dependent, endocrine-controlled later stage. We hypothesized that low...