A5100659690- Reproductive Biology and Fertility
- Organ Transplantation Techniques and Outcomes
- Ovarian function and disorders
- Liver Disease Diagnosis and Treatment
- Neuroblastoma Research and Treatments
- Hepatitis B Virus Studies
- Sperm and Testicular Function
- Liver Disease and Transplantation
- Hepatitis C virus research
- Advanced Radiotherapy Techniques
- Adipokines, Inflammation, and Metabolic Diseases
- Tissue Engineering and Regenerative Medicine
- Medical Imaging Techniques and Applications
- Cholesterol and Lipid Metabolism
- Lung Cancer Diagnosis and Treatment
- Renal Transplantation Outcomes and Treatments
- Pluripotent Stem Cells Research
- Cancer, Hypoxia, and Metabolism
- Vascular Malformations and Hemangiomas
- Cytomegalovirus and herpesvirus research
- Anesthesia and Neurotoxicity Research
- Radiomics and Machine Learning in Medical Imaging
- Tracheal and airway disorders
- Esophageal Cancer Research and Treatment
- Immune Cell Function and Interaction
Chinese Academy of Sciences
2020-2024
University of Science and Technology of China
2024
Fujian Medical University
2024
Regend Therapeutics (China)
2024
Union Hospital
2024
Shanghai Jiao Tong University
2009-2024
Tongren Hospital
2022-2024
University of Chinese Academy of Sciences
2024
Kunming Institute of Zoology
2024
University of Hong Kong
2024
Oocytes grown in vitro are of low quality and yield few live births, thus limiting the ability to store or bank ova women wishing preserve their fertility. We applied tissue engineering principles culture immature mouse follicles by designing an alginate hydrogel matrix maintain oocyte's 3- dimensional (3D) architecture cell-cell interactions vitro. A 3D mimics vivo follicle environment, hydrogel-encapsulated develop mature oocytes within capacity for fertilization similar that matured vivo....
BACKGROUNDYoung female adult and adolescent cancer patients facing life-preserving but fertility-threatening chemo- or radiation-therapy are increasingly seeking options to protect their reproductive potential. Ovarian tissue cryopreservation with transplantation is a promising technique safeguard fertility in patients. However, this method may risk re-introduction of the original survivor disease. Thus, developing for vitro growth immature follicles provide restoration future.
The availability of viable oocytes is the limiting factor in development new reproductive techniques. Many attempts have been made to grow immature vitro during recent decades. Recently, a modified alginate-based three-dimensional culture system was designed support growth and maturation multilayered secondary follicles. This able produce that successfully completed meiosis, fertilization, blastocyst stage. Subsequent two-layered follicles were unsuccessful under original conditions. Herein,...
In vitro ovarian follicle cultures may provide fertility-preserving options to women facing premature infertility due cancer therapies. An encapsulated three-dimensional (3-D) culture system utilizing biomaterials maintain cell-cell communication and support development produce a mature oocyte has been developed for the mouse. We tested whether this 3-D would also of nonhuman primate preantral follicles, which in growth not reported. Three questions were investigated: Does cycle stage at...
Abstract An option for fertility preservation women facing a cancer diagnosis involves the cryopreservation of ovarian tissue later re‐transplantation or in vitro culture, with culture preferred to avoid reintroduction cancer. Small, immature follicles survive freeze‐thaw process, and can be matured through follicle maturation (IFM) that an initial growth subsequent oocyte. The cryopreserved two forms: (i) cortical strips consisting surrounding stroma (Cryo‐Ov) (ii) individually isolated...
Ovarian cryopreservation before chemotherapy and autotransplantation post-treatment can restore fertility to women with premature ovarian failure. Although the majority of primordial follicles survive cycle, follicular pool is reduced after transplantation due ischemic death. Therefore, we engineered a biomaterial-based system promote angiogenesis in mouse model transplantation. To mimic clinical situation sterility, bilateral ovariectomy was performed 2 weeks transplantation, during which...
Steady-state alveolar macrophages (AMs) are long-lived lung-resident with sentinel function. Evidence suggests that AM precursors originate during embryogenesis and populate lungs without replenishment by circulating leukocytes. However, their presence persistence unclear following human lung transplantation (LTx). Our goal was to examine donor longevity evaluate whether AMs of recipient origin seed the transplanted lungs. Origin accessed using donor–recipient HLA mismatches. We demonstrate...
Folliculogenesis is a coordinated process, and the genes that regulate development are difficult to investigate in vivo. In vitro culture systems permit assessment of individual follicles during development, thereby enabling gene expression patterns be monitored follicle development. Mouse multilayered secondary (150-180 microm diameter) were cultured three-dimensional matrices varying physical properties for up 8 days. During this period growth vitro, antrum formation steroid production...
Donor organ shortages have led to an increased interest in finding new approaches recover organs from extended criteria donors (ECD). Normothermic extracorporeal liver perfusion (NELP) has been proposed as a superior preservation method reduce ischemia/reperfusion injury (IRI), precondition suboptimal grafts, and treat ECD livers so that they can be successfully used for transplantation. The aim of this study was investigate the beneficial effects modified NELP circuit on discarded human...
The roles of nuclear receptor subfamily 1 group d member (NR1D1) and the circadian clock in liver fibrosis remain unclear. Here, we showed that genes, especially NR1D1, were dysregulated mice with carbon tetrachloride (CCl4)-induced fibrosis. In turn, disruption exacerbated experimental NR1D1-deficient more sensitive to CCl4-induced fibrosis, supporting a critical role NR1D1 development. Validation at tissue cellular levels was primarily degraded by N6-methyladenosine (m6A) methylation...
Persons with chronic HBV infection coinfected HIV experience accelerated progression of liver fibrosis compared to those monoinfection. We aimed determine whether and its proteins promote HBV-induced in HIV/HBV-coinfected cell culture models through HIF-1α TGF-β1 signaling.
The ovarian follicle is the functional unit of ovary that secretes sex hormones and supports oocyte maturation. In vitro techniques provide a tool to model development in order investigate basic biology, are further being developed as technique preserve fertility clinic1-4. Our culture system employs hydrogels mimic native environment by maintaining 3D follicular architecture, cell-cell interactions paracrine signaling direct 5. Previously, follicles were successfully cultured alginate, an...
Alveolar macrophage (AM) is a mononuclear phagocyte key to the defense against respiratory infections. To understand AM's role in airway disease development, we examined influence of Secretoglobin family 1a member 1 (SCGB1A1), pulmonary surfactant protein, on AM development and function. In murine model, high-throughput RNA-sequencing gene expression analyses were performed purified AMs isolated from mice lacking Scgb1a1 compared with that expressing wild type at weaning (4 week), puberty (8...
BackgroundLymphatic malformations are common congenital vascular lesions. Neither surgical resection nor other treatments have been found to be effective for invasive cases. Recent research has suggested that sirolimus is in treating complex lymphatic (LMs). We aimed evaluate the effectiveness and safety of oral children living with LMs our hospital.MethodsFifty-six cases treated were collected from Shanghai Children's Medical Centre between June 2016 March 2019. All confirmed either by...
The chronic low-grade inflammation of adipose tissues, primarily mediated by tissue macrophages (ATMs), is the key pathogenic link between obesity and metabolic disorders. Oleanolic acid (OA) a natural triterpenoid possessing anti-diabetic anti-inflammation effects, but machinery poorly understood. This study investigated detailed mechanisms OA on in obese mice. C57BL/6J mice were fed with high-fat diet (HFD) for 12 weeks, then daily intragastric administrated vehicle, 25 50 mg/kg 4 weeks....
Ovarian follicle maturation results from a complex interplay of endocrine, paracrine, and direct cell-cell interactions. This study compared the dynamic expression key developmental genes during folliculogenesis in vivo vitro culture 3D alginate hydrogel system. Candidate gene profiles were measured within mouse two-layered secondary follicles, multi-layered cumulus-oocyte complexes (COCs). The 20 involved endocrine communication, growth signaling, oocyte development was investigated by...