Jalmagna is a popular deepwater rice variety with farmers of India because its good yield under waterlogged condition. However, the highly susceptible to bacterial blight (BB) disease. The development resistant cultivars has been most effective and economical strategy control disease situation. Three resistance genes (xa5 + xa13 Xa21) were transferred from Swarna BB pyramid line, using marker-assisted backcrossing (MAB) breeding strategy, into BB-susceptible elite cultivar, Jalmagna.

The immunological role of exosomes in allograft rejection remains unknown. We sought to determine whether are induced during lung and define the antigenic compositions HLA, lung-associated self-antigens (SAgs) microRNAs (miRNAs). Exosomes were isolated from sera bronchoalveolar lavage fluid 30 transplant recipients (LTxRs) who stable or had acute (AR) bronchiolitis obliterans syndrome (BOS). defined by flow cytometry for CD63 western blotting annexin V SAgs, collagen (Col-V) Kα1 tubulin...

Steady-state alveolar macrophages (AMs) are long-lived lung-resident with sentinel function. Evidence suggests that AM precursors originate during embryogenesis and populate lungs without replenishment by circulating leukocytes. However, their presence persistence unclear following human lung transplantation (LTx). Our goal was to examine donor longevity evaluate whether AMs of recipient origin seed the transplanted lungs. Origin accessed using donor–recipient HLA mismatches. We demonstrate...

Rice plants show yellowing, stunting, withering, reduced tillering and utimately low productivity in susceptible varieties under temperature stress. Comparative transcriptome analysis was performed to identify novel transcripts, gain new insights into different gene expression pathways involved cold tolerance rice. analyses of 5 treatments based on chilling stress exposure revealed more down regulated genes higher up tolerant genotypes. A total 13930 10599 differentially expressed (DEGs)...

High yielding rice varieties are usually low in grain iron (Fe) and zinc (Zn) content. These two micronutrients involved many enzymatic activities, lack of which cause disorders human body. Bio-fortification is a cheaper easier way to improve the content these nutrients grain.A population panel was prepared representing all phenotypic classes for Fe-Zn from 485 germplasm lines. The studied genetic diversity, structure association mapping milled rice. showed linkage disequilibrium showing...

Rice exhibits enormous genetic diversity, population structure and molecular marker-traits associated with abiotic stress tolerance to high temperature stress. A set of breeding lines landraces representing 240 germplasm were studied. Based on spikelet fertility percent under temperature, tolerant genotypes broadly classified into four classes. Genetic diversity indicated a moderate level base the for trait Wright's F statistic estimates showed deviation Hardy-Weinberg expectation in...

Rice crop is sensitive to cold stress at seedling stage. A panel of population representing 304 shortlisted germplasm lines was studied for stage chilling tolerance in indica rice. Six phenotypic classes were exposed six low temperature regimes under control phenotyping facility investigate response pattern. 66 genotypes all used ensuring genetic diversity, structure and association mapping the trait using 58 simple sequence repeat (SSR) 2 direct linked markers. moderate level diversity...

Alveolar macrophage (AM) is a mononuclear phagocyte key to the defense against respiratory infections. To understand AM's role in airway disease development, we examined influence of Secretoglobin family 1a member 1 (SCGB1A1), pulmonary surfactant protein, on AM development and function. In murine model, high-throughput RNA-sequencing gene expression analyses were performed purified AMs isolated from mice lacking Scgb1a1 compared with that expressing wild type at weaning (4 week), puberty (8...

The fall armyworm, Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae), is a serious pest known for its ability to feed on various crops, particularly maize, inflicting significant reduction in maize yield. Farmers have traditionally dependent chemical insecticides manage this pest. These are frequently ineffective because the pest’s hidden feeding habits hinder chemicals from reaching their intended target. In response challenge, an vitro bioassay was performed evaluate virulence of...

The pathogenesis of chronic rejection, Bronchiolitis Obliterans Syndrome (BOS) following lung transplantation (LT) is poorly understood. We hypothesized that development antibodies to HLA (DSA) associated with dysregulation microRNA (miRNA) predisposes BOS. Towards this, miRNA profiling mononuclear cells from 10 stable LT (DSA(-) BOS(-) ), DSA(+) (DSA group) and BOS(+) (BOS were performed. Prediction by mirPath indicated differential miRNAs in compared are significantly up-regulated...

Alveolar macrophages are terminally differentiated, lung-resident of prenatal origin. unique in their long life and important role lung development function, as well lung-localized responses to infection inflammation. To date, no unified method for identification, isolation, handling alveolar from humans mice exists. Such a is needed studies on these innate immune cells various experimental settings. The described here, which can be easily adopted by any laboratory, simplified approach...

Rice is the principal food crop for people in South and South-East Asia life millions of rural households worldwide. Bacterial blight (BB) a very common, widespread, highly destructive disease rice. By introduction nitrogen-responsive high-yielding rice varieties, there was many-fold increase productivity level tropical recent years. Continuous cropping with high nitrogen use general wet conditions aggravated incidence. The cost chemical control measures often shows adverse effects on...

Abstract Background Drought during reproductive stage is among the main abiotic stresses responsible for drastic reduction of grain yield in rainfed rice. The genetic mechanism drought tolerance very complex. Many physiological and morphological traits are associated with this stress tolerance. Robust molecular markers required detection incorporation these correlated into different superior backgrounds. Identification gene(s)/QTLs controlling its deployment rice improvement programs...

High seed vigour ensures good quality and higher productivity. Early seedling growth parameters indicate in rice. Seed via physiological is a complex trait controlled by many quantitative loci. A panel was prepared representing population of 274 rice landraces including genotypes from all the phenotypic groups sixseedling stage germination % for association mapping. Wide variations six studiedtraits were observed population. The classified into 3 genetic groups. Fixation indices indicated...

Protein, starch, amylose and total soluble sugars are basic metabolites of seed that influence the eating, cooking nutritional qualities rice. Chlorophyll is responsible for absorption utilization light energy influencing photosynthetic efficiency in rice plant. Mapping these traits very important detection more number robust markers improvement through molecular breeding approaches.A representative panel population was developed by including 120 germplasm lines from initial shortlisted 274...

Bacterial blight (BB) of rice caused by Xanthomonas oryzae pv. is a major disease in many growing countries. Pyramided lines carrying two BB resistance gene combinations (Xa21+xa13 and Xa21+xa5) were developed lowland cultivar Jalmagna background through backcross breeding integrating molecular markers. In each generation, markers closely linked to the genes used select plants possessing target genes. Background selection was continued those resistant until BC(3) generation. Plants having...

Abstract An FcR homolog (IpFcRI), representing the first such receptor from an ectothermic vertebrate, has been identified in channel catfish (Ictalurus punctatus). Mining of expressed sequence tag databases using mammalian sequences for CD16, CD32, and CD64 resulted identification a teleost Ig-binding receptor. IpFcRI is encoded by single-copy gene containing three Ig C2-like domains, but lacking transmembrane segment cytoplasmic tail. The domains are phylogenetically structurally related...

We investigated whether blockade of the CD47 signaling pathway could reduce ischemia-reperfusion injury (IRI) renal allografts donated after cardiac death (DCD) in a porcine animal model transplantation. Renal were subjected to 30 minutes warm ischemia, 3.5 hours cold and then perfused with humanized anti-CD47 monoclonal antibody (CD47mAb) treatment group or HTK solution control (n = 4/group). The animals euthanized five days At time reperfusion, indocyanine green-based vivo imaging showed...

Autoantibodies to the islet-specific Zn transporter ZnT8 (Slc30a8), as well CD4 T cells, have been identified in patients with type 1 diabetes. Here we examined for T-cell reactivity epitopes NOD mouse. Immunization a cytoplasmic domain of protein or peptides predicted bind I-A(g7) resulted response, indicating lack deletional tolerance. However, presentation by intraislet antigen-presenting cells (APC) was not detectable prediabetic mice. Presentation islet APC found only islets mice active...

Zbtb7a in alveolar macrophages influences early inflammation and autoimmunity antibody-mediated rejection of transplanted lungs.

Antioxidant-rich rice is a cheaper way to solve stress-related disorders and other health benefits for the global rice-eating population. Five antioxidant traits, namely, superoxide dismutase, flavonoids, anthocyanins, γ-oryzanol 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) activity were mapped using representative panel population through association mapping. Potential landraces carrying multiple compounds identified from The represented four genetic groups correspondence...

Abstract Immune responses to HLA and development of anti-donor (DSA) were shown play a role in chronic rejection following transplantation. We hypothesized that Abs MHC change microRNAs (miRNAs), leading lung allograft rejection. Microarray analysis was performed murine model anti-MHC–induced obliterative airway disease (OAD), correlate bronchiolitis. A unique profile dysregulated miRNAs detected OAD mice on days 7 15 after Ab administration compared with control. Sixty-seven increased 42...

The bacterial blight (BB) disease of rice is a major that reduces yield heavily in susceptible varieties. Ranidhan late maturing popular variety but shows high susceptibility to the disease. Two BB resistance genes were transferred into through marker-assisted backcross breeding approach. Tightly linked molecular markers deployed track plants carrying target each generation. Foreground screening detected 17, 16 and 15 progenies carry 3 BC1F1, BC2F1 BC3F1 generations, respectively. selected...