A5056718218- Transplantation: Methods and Outcomes
- Esophageal and GI Pathology
- Gastroesophageal reflux and treatments
- Extracellular vesicles in disease
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Organ Transplantation Techniques and Outcomes
- Tracheal and airway disorders
- Esophageal Cancer Research and Treatment
- Dysphagia Assessment and Management
- Mechanical Circulatory Support Devices
- Polyomavirus and related diseases
- Renal Transplantation Outcomes and Treatments
- Helicobacter pylori-related gastroenterology studies
- Eosinophilic Esophagitis
- Inflammatory mediators and NSAID effects
- Organ and Tissue Transplantation Research
- Medical Imaging and Pathology Studies
- Gastric Cancer Management and Outcomes
- Respiratory viral infections research
- MicroRNA in disease regulation
- Viral Infections and Immunology Research
- Neonatal Respiratory Health Research
- Cancer, Stress, Anesthesia, and Immune Response
- Pulmonary Hypertension Research and Treatments
- Pneumocystis jirovecii pneumonia detection and treatment
St. Joseph's Hospital and Medical Center
2016-2025
Creighton University
2009-2025
Phoenix (United States)
2018-2025
St. Joseph's Hospital
2015-2024
Dignity Health
2024
St. Joseph Hospital
2014-2021
St. Joseph Medical Center in Tacoma
2013-2021
University School
2021
Tucson Orthopaedic Institute
2017-2020
Cleveland Clinic
2018
Single-cell RNA sequencing provides new insights into pathologic epithelial and mesenchymal remodeling in the human lung.
Abstract Severe acute respiratory syndrome corona virus 2 (SARS-CoV-2) causes severe syndrome. mRNA vaccines directed at the SARS-CoV-2 spike protein resulted in development of Abs and protective immunity. To determine mechanism, we analyzed kinetics induction circulating exosomes with Ab following vaccination healthy individuals. Results demonstrated expressing on day 14 after followed by d second dose. Exosomes protein, to spike, T cells secreting IFN-γ TNF-α increased booster Transmission...
A series of radiolabeled cyclic arginine-glycine-aspartic acid (RGD) peptide ligands for cell adhesion molecule integrin alpha v beta 3-targeted tumor angiogenesis targeting are being developed in our laboratory. In this study, effort continues by applying a positron emitter 64Cu-labeled PEGylated dimeric RGD radiotracer 64Cu-DOTA-PEG-E[c(RGDyK)]2 lung cancer imaging. The indicated 3 avidity, but the PEGylation reduced receptor binding affinity ligand compared to unmodified dimer. revealed...
The immunological role of exosomes in allograft rejection remains unknown. We sought to determine whether are induced during lung and define the antigenic compositions HLA, lung-associated self-antigens (SAgs) microRNAs (miRNAs). Exosomes were isolated from sera bronchoalveolar lavage fluid 30 transplant recipients (LTxRs) who stable or had acute (AR) bronchiolitis obliterans syndrome (BOS). defined by flow cytometry for CD63 western blotting annexin V SAgs, collagen (Col-V) Kα1 tubulin...
Accumulation of senescent cells contributes to age-related diseases including idiopathic pulmonary fibrosis (IPF). Insulin-like growth factor binding proteins (IGFBPs) regulate many biological processes; however, the functional contributions IGFBP2 in lung remain largely unclear. Here, we report that intranasal delivery recombinant protects aged mice from weight loss and demonstrated antifibrotic effects after bleomycin injury. Notably, human-Igfbp2 transgenic reveal reduced senescence...
Abstract Circulating exosomes containing donor HLA and lung-associated self-antigens (SAg) are thought to play an important role in allograft rejection after human lung transplantation. We characterized isolated from serum of 10 transplant recipients (LTxR) diagnosed with bronchiolitis obliterans syndrome (BOS) compared them stable LTxR. Lung-associated SAg (K-α-1-tubulin [Kα1T] collagen V [Col-V]), MHC class II molecules, costimulatory molecules CD40, CD80, CD86, transcription factors...
Reports documenting the use of extracorporeal membrane oxygenation (ECMO) after blunt thoracic trauma are scarce. We used a large, multicenter database to examine outcomes when ECMO was in treating patients with trauma.We performed retrospective analysis Extracorporeal Life Support Organization between 1998 and 2014. The diagnostic code for pulmonary contusion (861.21, DRG International Classification Diseases-9th Rev.) identify treated trauma. Variations pre-ECMO respiratory support were...
Abstract The coronavirus disease 2019 (COVID-19) global pandemic is caused by severe acute respiratory syndrome 2 (SARS-CoV-2). However, our understanding of SARS-CoV-2-induced inflammation in alveolar epithelial cells remains very limited. contributions intracellular insulin-like growth factor binding protein-2 (IGFBP2) to SARS-CoV-2 pathogenesis are also unclear. In this study, we have uncovered a critical role for IGFBP2, specifically type (AEC2), the immunopathogenesis COVID-19. Using...
Lung cancer is the leading cause of deaths worldwide; approximately 85% these cancers are non-small cell lung (NSCLC). Patients with NSCLC frequently have tumors harboring somatic mutations in epidermal growth factor receptor (EGFR) gene that constitutive activation. These patients best clinical response to EGFR tyrosine kinase inhibitors (TKIs). Herein, we show fibroblast factor-inducible 14 (Fn14; TNFRSF12A) overexpressed tumors, and Fn14 levels correlate p-EGFR expression. We also report...
Abstract Pulmonary fibrosis is a form of chronic lung disease characterized by pathologic epithelial remodeling and accumulation extracellular matrix. In order to comprehensively define the cell types, mechanisms mediators driving fibrotic in lungs with pulmonary fibrosis, we performed single-cell RNA-sequencing suspensions from 10 non-fibrotic control 20 PF lungs. Analysis 114,396 cells identified 31 distinct types. We report remarkable shift phenotypes occurs peripheral PF, identify...