A5015880946- Transplantation: Methods and Outcomes
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Immune Cell Function and Interaction
- Organ Transplantation Techniques and Outcomes
- IL-33, ST2, and ILC Pathways
- Polyomavirus and related diseases
- Renal Transplantation Outcomes and Treatments
- Cancer Immunotherapy and Biomarkers
- Tracheal and airway disorders
- Immunotherapy and Immune Responses
- Mechanical Circulatory Support Devices
- Influenza Virus Research Studies
- Respiratory Support and Mechanisms
- Eosinophilic Esophagitis
- Asthma and respiratory diseases
- Respiratory viral infections research
- Pulmonary Hypertension Research and Treatments
- Esophageal and GI Pathology
- Medical Imaging and Pathology Studies
- Peptidase Inhibition and Analysis
- T-cell and B-cell Immunology
- Organ and Tissue Transplantation Research
- Immune cells in cancer
- CAR-T cell therapy research
- Airway Management and Intubation Techniques
St. Joseph's Hospital
2017-2024
St. Joseph's Hospital and Medical Center
2017-2024
Discovery Institute
2022-2024
Sanford Burnham Prebys Medical Discovery Institute
2022-2024
Australian National University
2017-2022
Albany Medical Center Hospital
2020-2022
Mayo Clinic in Arizona
2021
Christian Medical College & Hospital
2020
College of Medicine & JNM Hospital
2018
University of Arizona Cancer Center
2017
Abstract Many of the biological properties C5a are mediated through activation its receptor (C5aR1), expression which has been demonstrated convincingly on myeloid cells, such as neutrophils, monocytes, and macrophages. In contrast, conflicting results exist regarding C5aR1 in dendritic cells (DCs) lymphoid lineage cells. this article, we report generation a floxed GFP-C5aR1 reporter knock-in mouse. Using mouse strain, confirmed strong neutrophils from bone marrow, blood, lung, spleen, well...
PSGL-1 (P-selectin glycoprotein-1) is a T cell-intrinsic checkpoint regulator of exhaustion with an unknown mechanism action. Here, we show that acts upstream PD-1 and requires co-ligation the cell receptor (TCR) to attenuate activation mouse human CD8+ cells drive terminal exhaustion. directly restrains TCR signaling via Zap70 maintains expression inhibitor Sts-1. deficiency empowers respond low-affinity ligands inhibit growth PD-1-blockade-resistant melanoma by enabling tumor-infiltrating...
Abstract This study demonstrates that 24 h following viral vector-based vaccination IL-13Rα2 functions as a master sensor on conventional dendritic cells (cDCs), abetted by high protein stability coupled with minimal mRNA expression, to rapidly regulate DC mediated IL-13 responses at the lung mucosae, unlike IL-13Rα1. Under low IL-13, performs primary signalling receptor, whilst under acts sequester maintain homeostasis, both in STAT3-dependent manner. Likewise, we show vector-derived levels...
Pulmonary artery pseudoaneurysm (PAPA), an uncommon complication of pyogenic bacterial and fungal infections related septic emboli, is associated with high mortality. The pulmonary (PA) lacks adventitial wall; therefore, repeated endovascular seeding the PA emboli creates saccular dilations that are more likely to rupture than systemic arterial aneurysms. most common clinical presentation PAPA massive hemoptysis resultant worsening hypoxemia. Computed tomography angiography preferred...
Abstract IL-4 production is associated with low-avidity, poorly cytotoxic T cell induction that contributes to viral immune evasion and the failure of cell-based vaccines. Yet, precise mechanisms regulate signalling in cells remain elusive. Mounting evidence indicates can dynamically alter their IL-4/IL-13 receptor signature modulate downstream outcomes upon pathogen encounter. Here, we describe how naïve (CD62L + CD44 lo–mid ) CD4 CD8 distinctly engage both STAT6 STAT3 response IL-4. We...
This study demonstrates that route and viral vector can significantly influence the innate lymphoid cells (ILC) dendritic (DC) recruited to vaccination site, 24 h post delivery. Intranasal (i.n.) induced ST2/IL-33R+ ILC2, whilst intramuscular (i.m.) IL-25R+ TSLPR+ (Thymic stromal lymphopoietin protein receptor) ILC2 subsets. However, in muscle a novel ILC subset devoid of known markers (IL-25R− IL-33R− TSLPR−) were found express IL-13, unlike lung. Different vectors also influenced...
Interleukin-33 (IL-33) is a multifunctional cytokine that mediates type 2-dominated immune responses. In contrast, the role of IL-33 during viral vaccination, which often aims to induce 1 immunity, has not been fully investigated. Here, we examined effects on influenza vaccine We found intranasal coadministration with an inactivated virus increases efficacy against infection, only homologous strain but also heterologous strains, including 2009 H1N1 pandemic strain. Cross-protection was...
During the previous influenza seasons, between 2010 and 2016, live attenuated vaccine (LAIV) provided variable efficacy against in U.S., causing recommendation use of LAIV. In striking contrast, pre-clinical studies have repeatedly demonstrated superior LAIV mismatched viruses, compared to inactivated vaccines (IIV). This disparity reported efficacies clinical may part be explained by limitations animal models influenza. particular, absence pre-existing immunity has recently emerged as a...
Diaphragmatic paralysis, a known cause of dyspnoea, can drastically reduce breathing efficiency, diminishing quality life. We report our 3.5-year experience with 22 consecutive patients who underwent transabdominal, robot-assisted diaphragmatic plication for paralysis.We retrospectively reviewed this procedure by single surgeon from 5 September 2012 to 12 May 2016. The primary outcome measure was change in dyspnoea severity, which measured the 5-point Medical Research Council scale (a score...
Abstract In this study, recombinant pox viral vaccination was shown to induce highly elevated IgG2a and low IgG1 antibody expression in mice lacking IL‐4 or STAT6, whilst IL‐13 −/− exhibited IgG1, but very IgG2a. These findings revealed that differentially regulated development. To understand further, when STAT6 were given a vaccine co‐expressing IL‐13Rα2 temporarily sequestered IL‐13, significantly reduced expression, detected. for the first time demonstrated differentiation utilising an...
Background Preoperative atrial fibrillation is associated with poor outcomes after cardiac surgery, but its effect on lung transplantation remains unknown. Methods We retrospectively reviewed the charts of 235 patients who underwent in our institution from 2013 to 2015, analyzing demographics, length stay, survival, readmissions, and events. Mean recipient age was 59 ± 11 years, 142 (60.4%) were men. Patients grouped according pre-transplantation status (atrial fibrillation/no fibrillation)....
The proportion of lung transplant (LTx) recipients older than 70 years is increasing, thus we assessed long-term survival after LTx in this cohort relative to younger counterparts.We retrospectively reviewed charts patients who underwent between 2012 and 2016 at our center divided by age: group A (<65 years), B (65-69 C (≥70 years). Survival statistics were evaluated using the Kaplan-Meier method Cox regression.The study included 375 recipients: 221 (58.9%) A, 109 (29.1%) B, 45 (12.0%) C....
Lung nodules are common in lung transplant recipients (1), but the immunosuppressed status of these patients means that diagnosing is urgent. Differential diagnoses with include infection (i.e., bacterial, fungal, mycobacterial), malignancy [e.g., bronchogenic, post-transplant lymphoproliferative disorder (PTLD), or metastatic malignancy], and hemorrhage after previous transbronchial biopsies (1,2).
Immunosuppression after lung transplantation may increase susceptibility to opportunistic infection and is associated with early delayed deaths in transplant recipients. Factors that predispose recipients bacterial fungal infections include prolonged corticosteroid use, renal impairment, treatment of acute rejection, post-transplant diabetes mellitus. We present a unique case 63-year-old woman mellitus who underwent redo transplantation. Three years her right-sided single transplant, she...
The efficacy of the intranasally (i.n.) delivered live attenuated influenza vaccine (LAIV) is variable and, in some seasons, suboptimal. In this study, we report that LAIV exhibits cross-protective mice, potentially associated with cellular immunity as opposed to antigen-specific antibody responses. However, pre-exposure intramuscularly (i.m.) inactivated (IIV) severely impaired LAIV-induced cross-protection against heterologous challenge, by inhibiting replication LAIV. Our findings suggest...