A5058215158- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- HIV Research and Treatment
- SARS-CoV-2 and COVID-19 Research
- T-cell and B-cell Immunology
- Hepatitis C virus research
- Animal Virus Infections Studies
- Hepatitis B Virus Studies
- vaccines and immunoinformatics approaches
- Influenza Virus Research Studies
- Respiratory viral infections research
- Viral gastroenteritis research and epidemiology
- IL-33, ST2, and ILC Pathways
- Viral Infections and Immunology Research
- Bacterial Infections and Vaccines
- interferon and immune responses
- Eosinophilic Esophagitis
- Neuroinflammation and Neurodegeneration Mechanisms
- Immune Response and Inflammation
- Viral Infections and Vectors
- Cytomegalovirus and herpesvirus research
- Long-Term Effects of COVID-19
- COVID-19 Clinical Research Studies
- Monoclonal and Polyclonal Antibodies Research
- Virus-based gene therapy research
Vaxxas (Australia)
2023-2025
The University of Queensland
2020-2024
Basil Hetzel Institute
2014-2020
The University of Adelaide
2014-2020
Australian National University
2010-2019
Coronavirus disease-2019 (COVID-19) is primarily a respiratory disease, however, an increasing number of reports indicate that SARS-CoV-2 infection can also cause severe neurological manifestations, including precipitating cases probable Parkinson's disease. As microglial NLRP3 inflammasome activation major driver neurodegeneration, here we interrogated whether promote activation. Using transgenic mice expressing human angiotensin-converting enzyme 2 (hACE2) as COVID-19 pre-clinical model,...
A novel T cell–based ZIKV vaccine, encoding NS1 protein, confers protection against systemic infection.
Abstract The introduction of directly acting antiviral agents (DAAs) has produced significant improvements in the ability to cure chronic hepatitis C infection. However, with over 2% world’s population infected HCV, complications arising from development cirrhosis liver, infection remains leading indication for liver transplantation. Several modelling studies have indicated that DAAs alone will not be sufficient eliminate but if combined an effective vaccine this regimen would provide a...
Efforts to develop and deploy effective vaccines against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continue at pace. Here, we describe rational antigen design through manufacturability vaccine efficacy of a prefusion-stabilised spike (S) protein, Sclamp, in combination with the licensed adjuvant MF59 'MF59C.1' (Seqirus, Parkville, Australia).A panel recombinant Sclamp proteins were produced Chinese hamster ovary screened vitro select lead candidate. The structure this was...
The ongoing SARS-CoV-2 pandemic continues to be a major health burden globally, especially in resource-limited areas. Continued research into more effective and accessible vaccines is required reduce the of disease. Here, we use an emerging vaccine delivery system, high-density microarray patch (HD-MAP) deliver plasmid DNA (Delta 6P) encoding for spike protein. HD-MAP this resulted robust IgG responses mice against multiple domains cellular response vaccination was also measured, comparative...
Primary liver cancer is the sixth most prevalent globally and often diagnosed late, when treatment options are limited. Secondary cancer, arising from metastasis of other cancers to liver, a common complication advanced solid significant cause cancer-related morbidity mortality. Existing for primary secondary tumours have limited efficacy new modalities potential improve patient outcomes. mRNA therapeutics readily delivered healthy after systemic administration, but their uptake expression...
Hepatitis C virus (HCV) persistently infects approximately 71 million people globally. To prevent infection a vaccine which elicits neutralizing antibodies against the envelope proteins (E1/E2) are required for entry into host cells is desirable. DNA vaccines cost-effective to manufacture globally and despite recent landmark studies highlighting therapeutic efficacy of in humans cervical cancer, encoding E1/E2 developed thus far poorly immunogenic. We now report novel highly immunogenic...
Psoriasis is a common chronic inflammatory skin condition manifested by T cell responses and characterized preferential recurrence at previously inflamed sites upon withdrawal of treatment. The site-specific disease memory in psoriasis has been linked to CD8+CD103+ tissue-resident cells (Trm) the epidermis which were thought only provide “frontline” protection against pathogens immunosurveillance during cancer development. In this study, we correlated presence subset Trm are also CD49a+ with...
With the hope of understanding how interleukin (IL)-4 and IL-13 modulated quality anti-viral CD8+ T cells, we evaluated expression receptors for these cytokines following a range viral infections (e.g. pox viruses influenza virus). Results clearly indicated that unlike other IL-4/IL-13 receptor subunits, IL-4 α (IL-4Rα) was significantly down-regulated on cells in cognate antigen dependent manner. The infection gene knockout mice wild-type (WT) with vaccinia virus (VV) or VV expressing...
ABSTRACT There are 3 to 4 million new hepatitis C virus (HCV) infections annually around the world, but no vaccine is available. Robust T-cell mediated responses necessary for effective clearance of virus, and DNA vaccines result in a cell-mediated bias. Adjuvants often required vaccination, during natural lytic viral damage-associated molecular patterns (DAMPs) released, which act as adjuvants. Hence, that induces cell necrosis releases DAMPs will immunity (CMI), similar resulting from...
A universal hepatitis C virus (HCV) vaccine should elicit multiantigenic, multigenotypic responses, which are more likely to protect against challenge with the range of genotypes and subtypes circulating in community. cocktail vaccines encoding consensus HCV sequences attractive approaches achieve this goal. Consequently, a series mouse vaccination studies, we compared immunogenicity DNA nonstructural 5B (NS5B) protein that plasmids genotype 1b (Gt1b) Gt3a NS5B proteins. To complement study,...
The COVID-19 pandemic has highlighted the need for vaccines capable of providing rapid and robust protection. One way to improve vaccine efficacy is delivery via microarray patches, such as Vaxxas high-density patch (HD-MAP). We have previously demonstrated that a SARS-CoV-2 protein candidate, HexaPro, HD-MAP induces potent humoral immune responses. Here, we investigate cellular responses induced by HexaPro vaccination. found type one biassed response much greater magnitude compared standard...
Here we describe a multiplex, fluorescence-based, in vivo cytotoxic T-cell assay using the three vital dyes carboxyfluorescein diacetate succinimidyl ester, cell trace violet, and proliferation dye efluor 670. When used to label cells combination, these can discriminate >200 different target populations one animal due each population having unique fluorescence signature based on intensity emission wavelengths of dyes. This allows simultaneous measurement killing pulsed with numerous peptides...
Qualitative characteristics of cytotoxic CD8+ T cells (CTLs) are important in measuring the effectiveness CTLs controlling HIV-1 infections. Indeed, recent studies patients who naturally resistant to infections have been shown possess that high functional avidity and a capacity recognize HIV epitope variants, when compared infection progressors. When developing efficacious vaccines, assays can effectively measure CTL quality specifically vivo becoming increasingly important. Here we report...
This study demonstrates that route and viral vector can significantly influence the innate lymphoid cells (ILC) dendritic (DC) recruited to vaccination site, 24 h post delivery. Intranasal (i.n.) induced ST2/IL-33R+ ILC2, whilst intramuscular (i.m.) IL-25R+ TSLPR+ (Thymic stromal lymphopoietin protein receptor) ILC2 subsets. However, in muscle a novel ILC subset devoid of known markers (IL-25R− IL-33R− TSLPR−) were found express IL-13, unlike lung. Different vectors also influenced...
Subunit vaccines exhibit favorable safety and immunogenicity profiles can be designed to mimic native antigen structures. However, pairing with an appropriate adjuvant is imperative in order elicit effective humoral cellular immune responses. In this study, we aimed determine optimal the prefusion form of influenza haemagglutinin (HA) or respiratory syncytial virus (RSV) fusion (F) subunit BALB/c mice inform future vaccine selection. We tested a panel adjuvants, including aluminum hydroxide...
Abstract DNA vaccines are cost-effective to manufacture on a global scale and Tat-based have yielded protective outcomes in preclinical clinical models of human immunodeficiency virus (HIV), highlighting the potential such vaccines. However, been poorly immunogenic despite administration multiple doses and/or addition adjuvants, these not general use. In this study, we improved Tat immunogenicity by fusing it with oligomerisation domain chimeric C4-binding protein (C4b-p), termed IMX313,...
During the previous influenza seasons, between 2010 and 2016, live attenuated vaccine (LAIV) provided variable efficacy against in U.S., causing recommendation use of LAIV. In striking contrast, pre-clinical studies have repeatedly demonstrated superior LAIV mismatched viruses, compared to inactivated vaccines (IIV). This disparity reported efficacies clinical may part be explained by limitations animal models influenza. particular, absence pre-existing immunity has recently emerged as a...