A5066148125- Influenza Virus Research Studies
- Respiratory viral infections research
- SARS-CoV-2 and COVID-19 Research
- Viral gastroenteritis research and epidemiology
- Virology and Viral Diseases
- Animal Virus Infections Studies
- Animal Disease Management and Epidemiology
- Viral Infections and Vectors
- Immune Response and Inflammation
- Vaccine Coverage and Hesitancy
- Immune responses and vaccinations
- COVID-19 Impact on Reproduction
- Immunotherapy and Immune Responses
- vaccines and immunoinformatics approaches
- Infant Nutrition and Health
- Viral Infections and Immunology Research
- Poxvirus research and outbreaks
- COVID-19 Clinical Research Studies
- Mosquito-borne diseases and control
- HIV Research and Treatment
- Bacillus and Francisella bacterial research
- interferon and immune responses
- RNA and protein synthesis mechanisms
- Neonatal Respiratory Health Research
- Monoclonal and Polyclonal Antibodies Research
Wageningen University & Research
2020-2023
Viroclinics Biosciences (Netherlands)
2013-2022
University College Dublin
2022
Erasmus MC
2002-2015
Erasmus University Rotterdam
1999-2013
Codarts Rotterdam
2010
National Institute for Public Health and the Environment
1999-2002
Institute of Virology of the Slovak Academy of Sciences
2000
Transmission of severe acute respiratory coronavirus-2 (SARS-CoV-2) between livestock and humans is a potential public health concern. We demonstrate the susceptibility rabbits to SARS-CoV-2, which excrete infectious virus from nose throat upon experimental inoculation. Therefore, investigations on presence SARS-CoV-2 in farmed should be considered.
The use of classical smallpox vaccines based on vaccinia virus (VV) is associated with severe complications in both naive and immune individuals. Modified Ankara (MVA), a highly attenuated replication-deficient strain VV, has been proven to be safe humans immunocompromised animals, its efficacy against currently being addressed. Here we directly compare the efficacies MVA alone combination VV-based cynomolgus macaque monkeypox model. MVA-based vaccine protected macaques lethal respiratory...
Unprecedented spread between birds and mammals of highly pathogenic avian influenza viruses (HPAI) the H5N1 subtype has resulted in hundreds human infections with a high fatality rate. This highlighted urgent need for development vaccines that can be produced rapidly sufficient quantities. Potential pandemic inactivated will ideally induce substantial intra-subtypic cross-protection humans to warrant option use, either prior or just after start outbreak. In present study, we evaluated split...
The newly emerged influenza A(H1N1) virus (new H1N1 virus) is causing the first pandemic of this century. Three pandemics previous century caused variable mortality, which largely depended on development severe pneumonia. However, ability new to cause pneumonia poorly understood.The was inoculated intratracheally into ferrets. Its compared with that seasonal and highly pathogenic avian (HPAI) H5N1 by using clinical, virological, pathological analyses.Our results showed causes in ferrets...
Immunocompromised individuals tend to suffer from influenza longer with more serious complications than otherwise healthy patients. Little is known about the impact of prolonged infection and efficacy antiviral therapy in these Among all 189 A virus infected immunocompromised patients admitted ErasmusMC, 71 were hospitalized, since start 2009 H1N1 pandemic. We identified 11 (15%) cases pandemic replication (longer 14 days), despite therapy. In 5 out (45%) oseltamivir resistant H275Y viruses...
Background: Since the outbreak of coronavirus disease 2019 (COVID-19), many put their hopes in rapid availability effective immunizations. Human milk, containing antibodies against syndrome 2 (SARS-CoV-2), may serve as means protection through passive immunization. We aimed to determine presence and pseudovirus neutralization capacity SARS-CoV-2 specific IgA human milk mothers who recovered from COVID-19, effect pasteurization on these antibodies. Methods: This prospective case control study...
Humans may be infected by different influenza A viruses—seasonal, pandemic, and zoonotic—which differ in presentation from mild upper respiratory tract disease to severe sometimes fatal pneumonia with extra-respiratory spread. Differences spatial temporal dynamics of these infections are poorly understood. Therefore, we inoculated ferrets seasonal H3N2, pandemic H1N1 (pH1N1), highly pathogenic avian H5N1 virus performed detailed virological pathological analyses at time points 0.5 14 days...
Background The urgent medical need for innovative approaches to control influenza is emphasized by the widespread resistance of circulating subtype H1N1 viruses leading antiviral drug oseltamivir, pandemic threat posed occurrences human infections with highly pathogenic avian H5N1 viruses, and indeed evolving swine-origin pandemic. A recently discovered class monoclonal antibodies ability neutralize a broad spectrum (including H1, H2, H5, H6 H9 subtypes) has potential prevent treat in...
ABSTRACT The ability of Middle East respiratory syndrome coronavirus (MERS-CoV) to infect small animal species may be restricted given the fact that mice, ferrets, and hamsters were shown resist MERS-CoV infection. We inoculated rabbits with MERS-CoV. Although virus was detected in lungs, neither significant histopathological changes nor clinical symptoms observed. Infectious virus, however, excreted from upper tract, indicating a potential route transmission some species.
Ferrets are widely used as a small animal model for number of viral infections, including influenza A virus and SARS coronavirus. To further analyze the microbiological status ferrets, their fecal flora was studied using metagenomics approach. Novel viruses from families Picorna-, Papilloma-, Anelloviridae well known Astro-, Corona-, Parvo-, Hepeviridae were identified in different ferret cohorts. Ferret kobu- hepatitis E mainly present human household whereas coronaviruses found both farm...
Thrombocytopenia is a common complication of influenza virus infection, and its severity predicts the clinical outcome critically ill patients. The underlying cause(s) remain incompletely understood. In this study, in patients with an A/H1N1 viral load platelet count correlated inversely during acute infection phase. We confirmed finding ferret model infection. these animals, decreased degree pathogenicity varying from 0% animals infected A/H3N2 virus, to 22% those pandemic up 62% highly...
Efforts to develop and deploy effective vaccines against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continue at pace. Here, we describe rational antigen design through manufacturability vaccine efficacy of a prefusion-stabilised spike (S) protein, Sclamp, in combination with the licensed adjuvant MF59 'MF59C.1' (Seqirus, Parkville, Australia).A panel recombinant Sclamp proteins were produced Chinese hamster ovary screened vitro select lead candidate. The structure this was...
Previously we have shown that a single dose of recombinant adenovirus serotype 26 (Ad26) vaccine expressing prefusion stabilized SARS-CoV-2 spike antigen (Ad26.COV2.S) is immunogenic and provides protection in Syrian hamster non-human primate infection models. Here, investigated the immunogenicity, protective efficacy, potential for vaccine-associated enhanced respiratory disease (VAERD) mediated by Ad26.COV2.S moderate challenge model, using currently most prevalent G614 variant. Vaccine...
ABSTRACT Recombinant modified vaccinia virus Ankara (MVA), encoding the measles (MV) fusion (F) and hemagglutinin (H) (MVA-FH) glycoproteins, was evaluated in an MV vaccination-challenge model with macaques. Animals were vaccinated twice absence or presence of passively transferred MV-neutralizing macaque antibodies challenged 1 year later intratracheally wild-type MV. After second vaccination MVA-FH, all animals developed MV-specific T-cell responses. Although MVA-FH slightly less effective...
Severe influenza is often associated with disease manifestations outside the respiratory tract. While proinflammatory cytokines can be detected in lungs and blood of infected patients, role extra-respiratory organs production unknown. Here, we show that both 2009 pandemic H1N1 A (H1N1) virus highly pathogenic avian (H5N1) induce expression tumor necrosis factor α, interleukin-6, interleukin-8 tract central nervous system. In addition, H5N1 induced heart, pancreas, spleen, liver, jejunum....
Human respiratory syncytial virus (HRSV) is an important cause of severe tract disease in immunocompromised patients. Animal models are indispensable for evaluating novel intervention strategies this complex patient population. To complement existing rodents and non-human primates, we have evaluated the potential benefits HRSV infection model ferrets (Mustela putorius furo). Nine- to 12-month-old HRSV-seronegative immunocompetent or were infected with a low-passage wild-type strain subgroup...
Abstract Globally accessible therapeutics against SARS-CoV-2 are urgently needed. Here, we report the generation of first anti-SARS-CoV-2 DARPin molecules with therapeutic potential as well rapid large-scale production capabilities. Highly potent multivalent low picomolar virus neutralization efficacies were generated by molecular linkage three different monovalent molecules. These target various domains spike protein, thereby limiting possible viral escape. Cryo-EM analysis individual...
Middle East respiratory syndrome coronavirus (MERS-CoV) is a WHO priority pathogen for which vaccines are urgently needed. Using an immune-focusing approach, we created self-assembling particles multivalently displaying critical regions of the MERS-CoV spike protein ─fusion peptide, heptad repeat 2, and receptor binding domain (RBD) ─ tested their immunogenicity protective capacity in rabbits. "plug-and-display" SpyTag/SpyCatcher system, coupled RBD to lumazine synthase (LS) producing...
Enterovirus A71 (EV-A71) can elicit a wide variety of human diseases such as hand, foot, and mouth disease severe or fatal neurological complications. It is not clearly understood what determines the virulence fitness EV-A71. has been observed that amino acid changes in receptor binding protein, VP1, resulting viral to heparan sulfate proteoglycans (HSPGs) may be important for ability EV-A71 infect neuronal tissue. In this study, we identified presence glutamine, opposed glutamic acid, at...
We report an outbreak of cowpox virus among monkeys at a sanctuary for exotic animals. Serologic analysis and polymerase chain reaction were performed on blood swab samples from different rodent species trapped the during outbreak. Sequence comparison serologic results showed that brown rats (Rattus norvegicus) transmitted to monkeys.