A5067643421- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Molecular Biology Techniques and Applications
- Monoclonal and Polyclonal Antibodies Research
- Metabolomics and Mass Spectrometry Studies
- Glioma Diagnosis and Treatment
- Immunotherapy and Immune Responses
- Multiple Myeloma Research and Treatments
- RNA Research and Splicing
- Glycosylation and Glycoproteins Research
- Advanced Biosensing Techniques and Applications
- RNA modifications and cancer
- HIV/AIDS drug development and treatment
- Multiple Sclerosis Research Studies
- S100 Proteins and Annexins
- Analytical Chemistry and Chromatography
- Cancer, Hypoxia, and Metabolism
- Prostate Cancer Treatment and Research
- HIV Research and Treatment
- Peptidase Inhibition and Analysis
- Pregnancy and preeclampsia studies
- Extracellular vesicles in disease
- Ocular Oncology and Treatments
- Lung Cancer Research Studies
- Alzheimer's disease research and treatments
Erasmus MC
2016-2025
Erasmus University Rotterdam
2014-2023
Erasmus MC Cancer Institute
2003-2023
Cancer Genomics Centre
2014-2023
University of Groningen
1987-2021
Digital Proteomics (United States)
2017
Center for Systems Biology
2016
Biomedical Primate Research Centre
2015
University Medical Center Groningen
2015
Radboud University Medical Center
2006-2014
Exosomes are membrane vesicles from endosomal origin secreted by various cells such as hematopoietic, epithelial, and tumor cells. contain specific antigens potentially useful for immunotherapeutic purposes. Our aim was to determine if exosomes present in human cancerous pleural effusions identify their proteomic content. were purified sucrose gradient ultracentrifugation, electron microscopy used check both concentration purity of exosomes. Proteins separated one-dimensional sodium dodecyl...
DNA double-strand break (DSB) repair by nonhomologous end joining (NHEJ) requires the assembly of several proteins on ends. Although biochemical studies have elucidated aspects NHEJ reaction mechanism, much less is known about in living cells, mainly because inability to visualize at damage. Here we provide evidence that a pulsed near IR laser can produce DSBs without any visible alterations nucleus, and show accumulate irradiated areas. The levels Ku accumulation diminished time, showing...
Recommendations about structuring proteomic biomarker studies should increase the probability that such markers will be clinically useful.
Background Current markers for prostate cancer, such as PSA lack specificity. Therefore, novel biomarkers are needed. Unfortunately, the complexity of body fluids often hampers biomarker discovery. An attractive alternative approach is isolation small vesicles, i.e. exosomes, ∼100 nm, which contain proteins that specific to tissue from they derived and therefore can be considered treasure chests disease-specific Materials Methods Exosomes were isolated 2 immortalized primary epithelial cells...
Glioblastoma multiforme (GBM) is the most common primary brain tumor and without exception lethal. GBMs modify immune system, which contributes to aggressive nature of disease. Particularly, cells monocytic lineage, including monocytes, macrophages microglia, are affected. We investigated influence GBM-derived extracellular vesicles (EVs) on phenotype cells. Proteomic profiling showed GBM EVs be enriched with proteins functioning in matrix interaction leukocyte migration. appeared skew...
The analysis of cerebrospinal fluid (CSF) is used in biomarker discovery studies for various neurodegenerative central nervous system (CNS) disorders. However, little known about variation CSF proteins and metabolites between patients without neurological A baseline a large number compounds appears to be lacking. To analyze the protein metabolite abundances well-defined individual samples undergoing routine, non-neurological surgical procedures, we determined by multiple analytical...
Experimental Autoimmune Encephalomyelitis (EAE) is the most commonly used animal model for Multiple Sclerosis (MScl). CSF metabolomics in an acute EAE rat was investigated using targetted LC-MS and GC-MS. Acute Lewis rats induced by co-injection of Myelin Basic Protein with Complete Freund's Adjuvant. samples were collected at two time points: 10 days after inoculation, which during onset disease, 14 peak disease. The obtained metabolite profiles from points development show profound...
Immunocompromised individuals tend to suffer from influenza longer with more serious complications than otherwise healthy patients. Little is known about the impact of prolonged infection and efficacy antiviral therapy in these Among all 189 A virus infected immunocompromised patients admitted ErasmusMC, 71 were hospitalized, since start 2009 H1N1 pandemic. We identified 11 (15%) cases pandemic replication (longer 14 days), despite therapy. In 5 out (45%) oseltamivir resistant H275Y viruses...
Abstract The development, homeostasis, and repair of intrahepatic extrahepatic bile ducts are thought to involve distinct mechanisms including proliferation maturation cholangiocyte progenitor cells. This study aimed characterize human organoids (ECO) using canonical Wnt-stimulated culture medium previously developed for (ICO). Paired ECO ICO were derived from common duct liver tissue, respectively. Characterization showed both organoid types highly similar, though some differences in size...
Pancreatic ductal adenocarcinoma (PDAC) is notorious for its poor prognosis even after curative resection. Responses to immunotherapy are rare and related inadequate T-cell priming. We previously demonstrated the potency of allogeneic lysate-dendritic cell (DC) vaccination in a preclinical model. Here we translate this concept patients.In phase I study, patients with resected PDAC were included when they no radiologic signs recurrence standard-of-care treatment. Allogeneic tumour...
The objective of this study was to identify differentially expressed and prognostically important genes in pediatric medulloblastoma ependymoma by Affymetrix microarray analysis. Among the most discriminative genes, three members SOX transcription factor family were expressed. Both SOX4 SOX11 significantly overexpressed (median, 11-fold 5-fold, respectively) compared with normal cerebellum. SOX9 had greater expression 16-fold) cerebellum (p<0.001 for all comparisons). differential confirmed...
To standardize the use of cerebrospinal fluid (CSF) for biomarker research, a set stability studies have been performed on porcine samples to investigate influence common sample handling procedures proteins, peptides, metabolites and free amino acids. This study focuses at effect proteins analyzed by applying label-free quantitation using microfluidics nanoscale liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (chipLC-MS) as well matrix-assisted laser desorption...
Tamoxifen resistance is a major cause of death in patients with recurrent breast cancer. Current clinical factors can correctly predict therapy response only half the treated patients. Identification proteins that are associated tamoxifen first step toward better prediction and tailored treatment In present study we intended to identify putative protein biomarkers indicative cancer using nano-LC coupled FTICR MS. Comparative proteome analysis was performed on approximately 5,500 pooled tumor...
Novel markers for prostate cancer (PCa) are needed because current established such as prostate-specific antigen lack diagnostic specificity and prognostic value. Proteomics analysis of serum from mice grafted with human PCa xenografts resulted in the identification 44 tumor-derived proteins. Besides secreted proteins we identified several cytoplasmic proteins, among which were most subunits proteasome. Native gel electrophoresis sandwich ELISA showed that these present proteasome complexes...
Clinical outcome of patients with triple-negative breast cancer (TNBC) is highly variable. This study aims to identify and validate a prognostic protein signature for TNBC reduce unnecessary adjuvant systemic therapy. Frozen primary tumors were collected from 126 lymph node–negative therapy–naive patients. These samples used global proteome profiling in two series: an in-house training (n = 63) multicenter test set. Patients who remained free distant metastasis minimum 5 years after surgery...
Liver transplantation is the only effective treatment for end-stage liver disease, but absolute donor shortage remains a limiting factor. Recent advances in tissue engineering focus on generation of native extracellular matrix (ECM) by decellularized complete livers animal models. Although proof concept has been reported human livers, this study aims to perform whole decellularization clinically relevant series using controlled machine perfusion. In study, we describe mild nondestructive...
The discovery of genes and molecular pathways involved in the formation brain metastasis would direct development therapeutic strategies to prevent this deadly complication cancer. By comparing gene expression profiles Estrogen Receptor negative (ER-) primary breast tumors between patients who developed organs other than brain, we found that T lymphocytes promote metastases. To functionally test ability cells metastasis, used an vitro blood–brain barrier (BBB) model. co-culturing with cancer...
Changes to extracellular matrix (ECM) structures are linked tumor cell proliferation and metastasis. We previously reported that naturally occurring peptides of collagen type I elevated in urine patients with colorectal liver metastasis (CRLM). In the present study, we took an MS-based proteomic approach identify specific types up-regulated CRLM tissues compared healthy, adjacent from same patients. found 19 22 collagen-α chains significantly (p < 0.05) healthy tissues. At least four were...