A5059318668- Cell Image Analysis Techniques
- 3D Printing in Biomedical Research
- Advanced Fluorescence Microscopy Techniques
- Cancer Cells and Metastasis
- Single-cell and spatial transcriptomics
- Microtubule and mitosis dynamics
- Cellular Mechanics and Interactions
- Spaceflight effects on biology
- Ubiquitin and proteasome pathways
- Autophagy in Disease and Therapy
- Liver physiology and pathology
- Genetics, Aging, and Longevity in Model Organisms
- Pancreatic function and diabetes
- Photosynthetic Processes and Mechanisms
- bioluminescence and chemiluminescence research
- Cancer-related Molecular Pathways
- DNA Repair Mechanisms
- Innovative Microfluidic and Catalytic Techniques Innovation
- Erythrocyte Function and Pathophysiology
- Glioma Diagnosis and Treatment
- Space Exploration and Technology
- Immunotherapy and Immune Responses
- interferon and immune responses
- Cancer, Hypoxia, and Metabolism
- Advanced Biosensing Techniques and Applications
Goethe University Frankfurt
2015-2025
Goethe Institute
2021
German Centre for Cardiovascular Research
2016
Ludwig-Maximilians-Universität München
2016
Institut de Biologie Moléculaire et Cellulaire
2014
European Molecular Biology Laboratory
2004-2010
European Molecular Biology Laboratory
2008-2009
Forschungszentrum Jülich
2003
University of Regensburg
1999-2001
We use single-particle tracking to study the elastic properties of single microtubules grafted a substrate. Thermal fluctuations free microtubule's end are recorded, in order measure position distribution functions from which we calculate persistence length with contour lengths between 2.6 and 48 micrometers. find vary by more than factor 20 over total range lengths. Our results support hypothesis that shearing protofilaments contributes significantly mechanics microtubules.
Cancer cells in poorly vascularized tumor regions need to adapt an unfavorable metabolic microenvironment. As distance from supplying blood vessels increases, oxygen and nutrient concentrations decrease cancer react by stopping cell cycle progression becoming dormant. cytostatic drugs mainly target proliferating cells, dormancy is considered as a major resistance mechanism this class of anti-cancer drugs. Therefore, substances that have the potential enhance cytostatic-based chemotherapy...
Abstract Background Organoids are morphologically heterogeneous three-dimensional cell culture systems and serve as an ideal model for understanding the principles of collective behaviour in mammalian organs during development, homeostasis, regeneration, pathogenesis. To investigate underlying organisation organoids, we imaged hundreds pancreas cholangiocarcinoma organoids parallel using light sheet bright-field microscopy up to 7 days. Results We quantified organoid at single-cell...
Abstract The development, homeostasis, and repair of intrahepatic extrahepatic bile ducts are thought to involve distinct mechanisms including proliferation maturation cholangiocyte progenitor cells. This study aimed characterize human organoids (ECO) using canonical Wnt-stimulated culture medium previously developed for (ICO). Paired ECO ICO were derived from common duct liver tissue, respectively. Characterization showed both organoid types highly similar, though some differences in size...
Abstract Spheroids are three-dimensional cellular models with widespread basic and translational application across academia industry. However, methodological transparency guidelines for spheroid research have not yet been established. The MISpheroID Consortium developed a crowdsourcing knowledgebase that assembles the experimental parameters of 3,058 published spheroid-related experiments. Interrogation this identified heterogeneity in setup spheroids. Empirical evaluation interlaboratory...
Abstract Glioblastoma (GBM), the most malignant primary brain tumor in adults, has poor prognosis irrespective of therapeutic advances due to its radio-resistance and infiltrative growth into tissue. The present study assessed functions putative druggability BRCA1-associated ATM activator 1 (BRAT1) as a crucial factor driving key aspects GBM, including enhanced DNA damage response migration. By stable depletion BRAT1 GBM glioma stem-like (GSC) cell lines, we observed delay double-strand...
Launching a new drug on the market is an extremely time-consuming and expensive process. The total costs from lab bench to patient's bedside are in range of $800 million for each compound. Innovative pre-clinical assays urgently needed select most promising candidates. High-throughput molecular screening does not provide information effects cellular functions. Testing animals expensive, ethically controversial, poorly predictive response humans. Conventional two-dimensional (2D) do...
Organoids are three-dimensional in vitro-grown cell clusters that recapitulate key features of native organs. In regenerative medicine, organoid technology represents a promising approach for the replacement severely damaged organs, such as pancreas patients with type 1 diabetes. Isolation human organoids (hPOs) chemically defined serum-free culture media would be major milestone this approach.Starting from discarded pancreatic tissues, we developed large-scale process obtaining clinically...
Thermal shape fluctuations of grafted microtubules were studied using high resolution particle tracking attached fluorescent beads. First mode relaxation times extracted from the mean square displacement in transverse coordinate. For shorter than $\ensuremath{\sim}10\text{ }\text{ }\ensuremath{\mu}\mathrm{m}$, found to follow an ${L}^{2}$ dependence instead ${L}^{4}$ as expected standard wormlike chain model. This length is shown result a complex bending stiffness which can be understood...
Abstract Three-dimensional multicellular aggregates such as spheroids provide reliable in vitro substitutes for tissues. Quantitative characterization of at the cellular level is fundamental. We present first pipeline that provides three-dimensional, high-quality images intact resolution and a comprehensive image analysis completes traditional segmentation by algorithms from other fields. The combines light sheet-based fluorescence microscopy optically cleared with automated nuclei (F score:...
The filamentous ascomycete Podospora anserina is a well-established aging model in which variety of different pathways, including those involved the control respiration, ROS generation and scavenging, DNA maintenance, proteostasis, mitochondrial dynamics, programmed cell death have previously been demonstrated to affect life span. Here we address potential role autophagy. We provide data demonstrating high basal autophagy levels even strains cultivated under noninduced conditions. By...
The AMP-activated protein kinase (AMPK) is stimulated by hypoxia, and although the AMPKα1 catalytic subunit has been implicated in angiogenesis, little known about role played AMPKα2 vascular repair.To determine of repair.Recovery blood flow after femoral artery ligation was impaired (>80%) AMPKα2-/- versus wild-type mice, a phenotype reproduced mice lacking myeloid cells (AMPKα2ΔMC). Three days ligation, neutrophil infiltration into ischemic limbs AMPKα2ΔMC lower than that despite being...