A5022278280- Colorectal Cancer Treatments and Studies
- Cancer Genomics and Diagnostics
- Cancer Research and Treatments
- Cancer Cells and Metastasis
- Genetic factors in colorectal cancer
- 3D Printing in Biomedical Research
- Colorectal and Anal Carcinomas
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Molecular Biology Techniques and Applications
- Pregnancy and preeclampsia studies
- Animal testing and alternatives
- Epigenetics and DNA Methylation
- Cell Image Analysis Techniques
- Pancreatic and Hepatic Oncology Research
- Birth, Development, and Health
- Inflammatory mediators and NSAID effects
- Radiomics and Machine Learning in Medical Imaging
- Advanced Fluorescence Microscopy Techniques
- Health and Medical Research Impacts
- Infections and bacterial resistance
- Celiac Disease Research and Management
- Liver physiology and pathology
- Fibroblast Growth Factor Research
- Growth Hormone and Insulin-like Growth Factors
- Ferroptosis and cancer prognosis
Université Paris Sciences et Lettres
2016-2024
Université Paris Cité
2009-2023
Institut Curie
2012-2023
Sorbonne Paris Cité
2014-2023
Délégation Paris 5
2008-2021
Institució Catalana de Recerca i Estudis Avançats
2017
Universitat Autònoma de Barcelona
2017
Université Paris 1 Panthéon-Sorbonne
2010
Centre National de la Recherche Scientifique
2005-2008
Hôpital René Huguenin
2008
Abstract Spheroids are three-dimensional cellular models with widespread basic and translational application across academia industry. However, methodological transparency guidelines for spheroid research have not yet been established. The MISpheroID Consortium developed a crowdsourcing knowledgebase that assembles the experimental parameters of 3,058 published spheroid-related experiments. Interrogation this identified heterogeneity in setup spheroids. Empirical evaluation interlaboratory...
A better understanding of the molecular profile anal squamous cell carcinomas (ASCCs) is necessary to consider new therapeutic approaches, and identification prognostic predictive factors for response treatment. We retrospectively analysed tumours from ASCC patients mutational analysis KRAS, NRAS, HRAS, BRAF, PIK3CA, MET, TP53 FBXW7 genes by HRM Sanger sequencing analysis. Specimens 148 were analysed: 96 treatment-naive 52 recurrences after initial radiotherapy (RT) or chemoradiotherapy...
A fully flexible imaging window acting as an implantable transparent “second skin” upgrades in vivo imaging.
The placenta relies on phenotypes that are characteristic of cancer to successfully implant the embryo in uterus during early pregnancy. Notably, it has invade its host tissues, promote angiogenesis-while surviving hypoxia-, and escape immune system. Similarities DNA methylation patterns between cancers suggest common epigenetic mechanisms may be involved regulating these behaviors. We show here megabase-scale hypomethylation distinguish first from third trimester chorionic villi placenta,...
RSPO ligands, activators of the Wnt/β-catenin pathway, are overexpressed in different cancers. The objective this study was to investigate role RSPOs breast cancer (BC). Expression and markers various pathways were measured tumours cell lines by qRT–PCR. effect on pathway activity determined luciferase assay, western blotting, RSPO2 inhibition proliferation using siRNAs. IWR-1, an inhibitor examined growth RSPO2-positive patient-derived xenograft (PDX) model metaplastic triple-negative BC....
Tumor endothelial transdifferentiation and VEGFR1/2 expression by cancer cells have been reported in glioblastoma but remain poorly documented for many other types. To characterize vasculature of patient-derived tumor xenografts (PDXs), largely used preclinical anti-angiogenic assays, we designed here species-specific real-time quantitative RT-PCR assays. Human mouse PECAM1/CD31, ENG/CD105, FLT1/VEGFR1, KDR/VEGFR2 VEGFA transcripts were analyzed a large series 150 PDXs established from 8...
Anal squamous cell carcinoma (ASCC) is a rare tumour, but its incidence increasing. Standard chemoradiotherapy fails to cure 20% of patients and no targeted therapy currently approved for recurrent ASCC. The PI3K/Akt/mTOR pathway frequently altered in this poorly characterised . IGF2 was identified here as key factor ASCC oncogenesis, shown play crucial role the PI3K with frequent (~60%) mutually exclusive genomic alterations IGF2, IGF1R, PTEN PIK3CA genes. We also demonstrated that...
Abstract The management of anal squamous cell carcinoma (ASCC) has yet to experience the transformative impact precision medicine. Conducting genomic analyses may uncover novel prognostic biomarkers and offer potential directions for development targeted therapies. To that end, we assessed theragnostic implications pathogenic variants identified in 571 cancer‐related genes from surgical samples collected a homogeneous, multicentric French cohort 158 ASCC patients who underwent...
Genomic alterations of anal squamous cell carcinoma (ASCC) remain poorly understood due to the rarity this tumor. Array comparative genomic hybridization and targeted gene sequencing were performed in 49 cases ASCC. The most frequently altered regions (with a frequency greater than 25%) 10 deleted (2q35, 2q36.3, 3p21.2, 4p16.3, 4p31.21, 7q36.1, 8p23.3, 10q23.2, 11q22.3, 13q14.11) 8 gained (1p36.33, 1q21.1, 3q26.32, 5p15.33, 8q24.3, 9q34.3, 16p13.3, 19p13.3). frequent minimal deletion (55%)...
Patient-derived tumor xenograft (PDX) is now largely recognized as a key preclinical model for cancer research, mimicking patient phenotype and genotype. Immunodeficient mice, well-known to develop spontaneous lymphoma, are required PDX growth. As all animal models used further clinical translation, robust experimental design strongly lead conclusive results. Here we briefly report unintentional co-engraftment of mouse lymphoma during expansion well-established PDXs illustrate the importance...
The sequence of the beta-subunit human chorionic gonadotropin (hCGβ) varies depending on whether hCGβ is encoded by type I or II genes. Type genes are upregulated in trophoblast and cancer but can be detected serum nonpregnant women healthy individuals. We aimed to determine monoclonal antibody (mAb) FBT11-II specifically detects (type hCGβ).Competitive inhibition assays with synthetic peptides, immunocytochemical immunohistochemical studies, dosing immunoassays sequencing CGB were...
Abstract Intravital microscopy (IVM) is a powerful technique that enables imaging of internal tissues at (sub)cellular resolutions in living animals. Here, we present silicone-based window consisting fully flexible, suture-less design ideally suited for long-term, longitudinal IVM growing and tumors. Crucially, show this window, without any customization, suitable numerous anatomical locations mice using rapid standardized implantation procedure. This low-cost device represents substantial...
Inactivation des gènes murins codant la chaîne lourde IgHInactivation légère Igκ
14515 Background: In vitro spheroid model using cancer cell lines is widely admitted to mimic in vivo micro tumors, including micrometastases. Floating cluster culture has been recently used for normal and stem expansion. Spontaneously spheroids generated have only studied ovarian ascites while organoid aggregates sometimes observed the establishment of human colon lines. this study, we investigated whether spontaneous from could be isolated characterized. Methods: 127 colorectal primary...
e15060 Background: A better understanding of the molecular profile anal squamous cell carcinomas (ASCCs) is necessary to consider new therapeutic approaches, and identification prognostic predictive factors for response treatment. Methods: We retrospectively analyzed tumors from ASCC patients mutational analysis KRAS, NRAS, HRAS, BRAF, PI3KCA, MET, TP53 FBXW7 genes. Results: Specimen 148 were analyzed: 96 treatment-naive 52 recurrences after initial radiotherapy (RT) or chemoradiotherapy...