A5070600467- Analytical Chemistry and Sensors
- 3D Printing in Biomedical Research
- Nanoplatforms for cancer theranostics
- Cancer, Hypoxia, and Metabolism
- Molecular Sensors and Ion Detection
- Advanced Fluorescence Microscopy Techniques
- Luminescence and Fluorescent Materials
- Mitochondrial Function and Pathology
- Cancer Cells and Metastasis
- Electrochemical sensors and biosensors
- Nitric Oxide and Endothelin Effects
- ATP Synthase and ATPases Research
- Advanced biosensing and bioanalysis techniques
- Optical Imaging and Spectroscopy Techniques
- Porphyrin and Phthalocyanine Chemistry
- Electrospun Nanofibers in Biomedical Applications
- Adipose Tissue and Metabolism
- Cell Image Analysis Techniques
- Tissue Engineering and Regenerative Medicine
- Bone Tissue Engineering Materials
- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
- Metabolomics and Mass Spectrometry Studies
- RNA and protein synthesis mechanisms
- Cancer Research and Treatments
Ghent University
2020-2025
Ghent University Hospital
2021-2024
University College Cork
2011-2020
Sechenov University
2018-2020
Lomonosov Moscow State University
2018-2020
Institute of Bioorganic Chemistry
2005-2016
Bellingham Technical College
2015
Russian Academy of Sciences
2005-2008
Institute of Bioorganic Chemistry
2007
Institute of Evolutionary Physiology and Biochemistry
1996
Abstract Monitoring cell and tissue oxygenation is important for the analysis of development differentiation, mitochondrial function, common (patho)physiological conditions such as ischemia, cancer, neurodegenerative disorders. A number materials sensing cellular oxygen (O 2 ) by optical means have been described in recent years, but diverse range biological models measurement tasks demands more versatile, flexible, simple O sensors. new cell‐penetrating phosphorescent nanosensor material...
High brightness, chemical and photostability, tunable characteristics, spectral surface properties are important attributes for nanoparticle probes designed live cell imaging. We describe a class of nanoparticles high-resolution imaging O2 that consists substituted conjugated polymer (polyfluorene or poly(fluorene-alt-benzothiadiazole)) acting as FRET antenna fluorescent reference with covalently bound phosphorescent metalloporphyrin (PtTFPP, PtTPTBPF). The prepared from such copolymers by...
Oxygen and glucose metabolism play pivotal roles in many (patho)physiological conditions. In particular, oxygen deprivation (OGD) during ischemia stroke results extensive tissue injury cell death. Using time-resolved ribosome profiling, we assess gene expression levels a neural line, PC12, the first hour of OGD. The most substantial alterations are seen to occur within 20 minutes While transcription only 100 genes is significantly altered one OGD, translation response affects approximately...
PtPFPP-carbohydrate conjugates are promising O<sub>2</sub>probes for 3D PLIM imaging of live spheroids and brain explants.
Stem cells and the niche in which they reside feature a complex microenvironment with tightly regulated homeostasis, cell-cell interactions dynamic regulation of metabolism. A significant number organoid models has been described over last decade, yet few methodologies can enable single cell level resolution analysis stem metabolic demands, real-time without perturbing integrity. Here, we studied redox metabolism Lgr5-GFP intestinal organoids by two emerging microscopy approaches based on...
Abstract Spheroids are three-dimensional cellular models with widespread basic and translational application across academia industry. However, methodological transparency guidelines for spheroid research have not yet been established. The MISpheroID Consortium developed a crowdsourcing knowledgebase that assembles the experimental parameters of 3,058 published spheroid-related experiments. Interrogation this identified heterogeneity in setup spheroids. Empirical evaluation interlaboratory...
Transient early endosome (EE)-mitochondria interactions can mediate mitochondrial iron translocation, but the associated mechanisms are still elusive. We showed that Divalent Metal Transporter 1 (DMT1) sustains translocation via EE-mitochondria in triple-negative MDA-MB-231, not luminal A T47D breast cancer cells. DMT1 silencing increases labile pool (LIP) levels and activates PINK1/Parkin-dependent mitophagy MDA-MB-231 Mitochondrial bioenergetics iron-associated protein profile were altered...
Assessment of hypoxia, nutrients, metabolite gradients, and other hallmarks the tumor microenvironment within 3D multicellular spheroid organoid models represents a challenging analytical task. Here, we report red/near-infrared (NIR) emitting cell staining with O2-sensitive nanoparticles, which enable measurements oxygenation on conventional fluorescence microscope. Nanosensor probes, termed "MMIR" (multimodal infrared), incorporate an NIR metalloporphyrin (PtTPTBPF) deep red aza-BODIPY...
Abstract Volumetric bioprinting has revolutionized the field of biofabrication by enabling creation cubic centimeter-scale living constructs at faster printing times (in order seconds). However, a key challenge remains: developing wider variety available osteogenic bioinks that allow maturation encapsulated cells within construct. Herein, bioink exploiting step-growth mechanism (norbornene-norbornene functionalized gelatin in combination with thiolated - GelNBNBSH) outperformed chain-growth...
The supply of oxygen (O(2)) to respiring tissue, cells, and mitochondria regulates metabolism, gene expression, cell fate. Depending on the type mitochondrial function, O(2) gradients between extra- intracellular compartments may vary play important physiological roles such as regulation activity prolyl hydroxylases adaptive responses hypoxia. Here we present a new methodology for analysis localized in cultures adherent using three phosphorescent Pt-porphyrin based probes with different...
Abstract Ir III –porphyrins are a relatively new group of phosphorescent dyes that have potential for oxygen sensing and labeling biomolecules. The requirement two axial ligands the ion permits simple linkage biomolecules by one‐step ligand‐exchange reaction, example, using precursor carbonyl chloride complexes peptides containing histidine residue(s). Using this approach, we produced three –octaethylporphyrin with cell‐penetrating (Ir1 Ir2) tumor‐targeting (Ir3) studied their photophysical...
A profound understanding of how to tailor surface topographies electrospun fibers is great importance for sensitive applications including optical sensing, catalysis, drug delivery and tissue engineering. Hereby, a novel approach comprehend the driving forces fiber topography formation introduced through inclusion dynamic solvent-polymer interaction during formation. Thus, interplay between polymer solubility as well computed jet temperature changes in function time solvent evaporation...
Live cells function within narrow limits of physiological temperature (T) and O2 metabolite concentrations. We have designed a cell-permeable T-sensitive fluorescence lifetime-based nanoprobe based on lipophilic sulforhodamine, which stains 2D 3D cell models, shows cytoplasmic localization, has robust response to T (∼0.037 ns/K). Subsequently, we evaluated the probe lifetime imaging microscopy (FLIM) technique for combined gradients in metabolically active cells. found that adherent culture...
ABSTRACT A major focus of current biological studies is to fill the knowledge gaps between cell, tissue and organism scales. To this end, a wide array contemporary optical analytical tools enable multiparameter quantitative imaging live fixed cells, three-dimensional (3D) systems, tissues, organs organisms in context their complex spatiotemporal molecular features. In particular, modalities luminescence lifetime imaging, comprising fluorescence (FLI) phosphorescence microscopy (PLIM),...
Abstract Luminescence thermometry represents a technique of choice for measurements in small objects and imaging temperature distribution. However, most state‐of‐the‐art luminescent probes are limited spectral characteristics, brightness, photostability, sensitivity. Molecular thermometers the new generation utilizing air moisture‐stable zirconium(IV) pyridinedipyrrolide complexes can address all these limitations. The dyes emit pure thermally activated delayed fluorescence without any...
Several new perylene bisimide (PBI) probes comprising oligo-guanidine conjugates and cationic hydrogel nanoparticle structures were designed for sensing intracellular pH in live cell fluorescence lifetime imaging microscopy (FLIM). Using adherent mammalian cells (2D) neurosphere (3D) models, we evaluated their performance by confocal FLIM-TCSPC. The PBI probe showed stable calibration changes from 4.7 to 3.7 ns between 4.4 8 attributed photo-induced electron transfer (PET). molecular fast...