A5005733713- Archaeological and Historical Studies
- Archaeological and Geological Studies
- 3D Printing in Biomedical Research
- Systemic Lupus Erythematosus Research
- Medieval Architecture and Archaeology
- Advanced Fluorescence Microscopy Techniques
- Immune cells in cancer
- Single-cell and spatial transcriptomics
- Cancer Cells and Metastasis
- Rheumatoid Arthritis Research and Therapies
- Muscle Physiology and Disorders
- Cancer, Hypoxia, and Metabolism
- T-cell and B-cell Immunology
- Melanoma and MAPK Pathways
- Spaceflight effects on biology
- Educational Games and Gamification
- Immune Cell Function and Interaction
- Autoimmune and Inflammatory Disorders Research
- Muscle metabolism and nutrition
- Osteoarthritis Treatment and Mechanisms
- Knee injuries and reconstruction techniques
- Diet and metabolism studies
- Digital Accessibility for Disabilities
- Mesenchymal stem cell research
- Nanoplatforms for cancer theranostics
Trinity College Dublin
2019-2024
Universidad de Granada
2022
Direção Geral do Património Cultural
2022
22q11 Ireland
2021
Polytechnic Institute of Porto
2020
University of Lisbon
2017
Stem cells and the niche in which they reside feature a complex microenvironment with tightly regulated homeostasis, cell-cell interactions dynamic regulation of metabolism. A significant number organoid models has been described over last decade, yet few methodologies can enable single cell level resolution analysis stem metabolic demands, real-time without perturbing integrity. Here, we studied redox metabolism Lgr5-GFP intestinal organoids by two emerging microscopy approaches based on...
Objectives Immune and stromal cell communication is central in the pathogenesis of rheumatoid arthritis (RA) psoriatic (PsA), however, nature these interactions synovial pathology two pathotypes can differ. Identifying immune-stromal crosstalk at site inflammation RA PsA challenging. This study creates first global transcriptomic analysis inflamed joint investigates inflammation. Methods Single profiling 178 000 tissue cells from five patients with four RA, importantly, without prior sorting...
While autoantibodies are used in the diagnosis of rheumatoid arthritis (RA), function B cells inflamed joint remains elusive. Extensive flow cytometric characterization and SPICE algorithm analyses single-cell synovial tissue from patients with RA revealed accumulation switched double-negative memory programmed death-1 receptor-expressing (PD-1-expressing) at site inflammation. Accumulation was mediated by CXCR3, evident observed increase CXCR3-expressing compared periphery, differential...
Metabolic reprogramming of innate immune cells is emerging as a key player in the progression number chronic diseases, including atherosclerosis, where high rates glycolysis correlate with plaque instability. This study aimed to investigate if cholesterol crystals, which are atherosclerosis-associated DAMPs (damage/danger-associated molecular patterns), alter cell metabolism and whether this, turn, impacts on macrophage phenotype function.Primary human macrophages were treated crystals...
Retinal degeneration is the leading cause of incurable blindness worldwide and characterised by progressive loss light-sensing photoreceptors in neural retina. SARM1 known for its role axonal degeneration, but a photoreceptor cell has not been reported. to mediate neuronal through depletion essential metabolite NAD induction energy crisis. Here, we demonstrate that expressed photoreceptors, using retinal tissue explant, confirm activation causes destruction pools layer. Through generation...
Since the recent observation that immune cells undergo metabolic reprogramming upon activation, there has been immense research in this area to not only understand basis of such changes, but also exploit rewiring for therapeutic benefit. In a resting state, macrophages preferentially utilise oxidative phosphorylation generate energy; however, presence cell activators, glycolytic genes are upregulated, and energy is generated through glycolysis. This facilitates rapid production biosynthetic...
Objectives This study investigates pathogenic and protective polyfunctional T-cell responses in patient with rheumatoid arthritis (RA), individuals at risk (IAR) healthy control (HC) synovial-tissue biopsies identifies the presence of a novel population T-cells that are enriched RA joint prior to development clinical inflammation. Methods Pathway enrichment analysis previously obtained RNAseq data synovial from (n=118), IAR (n=20) HC (n=44) was performed. Single-cell tissue suspensions...
The stem cell niche at the perivascular space plays a role in dictating fate of cells within it. This study predicts <italic>in silico</italic> and models space, miniaturised bioreactor, with non-invasive assessment metabolism.
In this study, we utilise fluorescence lifetime imaging of NAD(P)H-based cellular autofluorescence as a non-invasive modality to classify two contrasting states human macrophages by proxy their governing metabolic state. Macrophages derived from blood-circulating monocytes were polarised using established protocols and metabolically challenged small molecules validate responding actions in extracellular acidification oxygen consumption. Large field-of-view images individual obtained...
Abstract This study examines trends in infant diet, breastfeeding and weaning Portugal through time Roman, Medieval Muslim Christian skeletal assemblages (1st to the 15th century CE). New stable carbon (δ 13 C) nitrogen 15 N) measurements were collected from 143 non-adults 46 adults that are analysed alongside comparative published datasets contemporaneous Iberian populations. A statistical package was used model bone collagen isotope data of individuals, quantitatively estimating onset...
Malignant melanoma is among the tumor entities with highest increase of incidence worldwide. To elucidate progression and develop new effective therapies, rodent models are commonly used. While these do not adequately reflect human physiology, two-dimensional cell cultures lack crucial elements microenvironment. address this shortcoming, we have developed a skin equivalent based on an open-source epidermal model. Melanoma lines different driver mutations were incorporated into forming...
A strategy assisted by an inorganic template was developed to promote the organized self-assembly of meso-(tetrakis)-(p-sulfonatophenyl)porphyrin (TPPS) on pH-sensitive core-shell polyelectrolyte microcapsules (PECs) poly(styrenesulfonate) (PSS) and poly(allylamine hydrochloride) (PAH). key feature this is use CaCO3 microparticles as a nucleation site endorsing inside-outside directional growth porphyrin aggregates. Using approach, TPPS in positively charged PECs with (PAH/PSS)2PAH sequence...
Mechanical stimulation can modulate the chondrogenic differentiation of stem/progenitor cells and potentially benefit tissue engineering (TE) functional articular cartilage (AC). cues like hydrostatic pressure (HP) are often applied to cell-laden scaffolds, with little optimization other key parameters (e.g. cell density, biomaterial properties) known effect lineage commitment. In this study, we first sought establish seeding densities fibrin concentrations supportive robust chondrogenesis...
The extracellular parasite and causative agent of African sleeping sickness Trypanosoma brucei (T. brucei) has evolved a number strategies to avoid immune detection in the host. One recently described mechanism involves conversion host-derived amino acids aromatic ketoacids, which are detected at relatively high concentrations bloodstream infected individuals. These ketoacids have been shown directly suppress inflammatory responses murine cells, as well acting potent inducers stress response...
The paper presents the development of an inclusive serious multiplayer game with Morse code as its theme, well describing methodologies and architectures for developing a through specific concepts deaf (sign language) blind (audio description). preceding case has three modes: classic one versus one, cooperative mode between two players four-player all. In order to validate this performance quality, final product dimensions: pedagogical, ergonomic technical. From obtained data, it was...
This study performed an in-depth investigation into the myeloid cellular landscape in synovium of patients with rheumatoid arthritis (RA), “individuals at risk” RA, and healthy controls (HC). Flow cytometric analysis demonstrated presence a CD40-expressing CD206 + CD163 macrophage population dominating inflamed RA synovium, associated disease activity treatment response. In-depth RNA sequencing metabolic that this is transcriptionally distinct, displaying unique inflammatory tissue-resident...
Serious games have a great potential to help people developing new skills or improving previously existing ones. Deaf andblind community face considerable challenges and difficulties regarding the acquisition of in literacy. The designof
Abstract BackgroundThoroughbred racehorse performance is largely influenced by a major quantitative trait locus at the myostatin ( MSTN ) gene which determines aptitude for certain race distances due to promoter region insertion mutation influencing functional phenotypes in skeletal muscle. To develop an vitro system experiments we established three novel equine muscle cell lines reflecting variation phenotype associated with genotype (CC/II, CT/IN and TT/NN SNP g.66493737C>T/SINE 227 bp...
Thoroughbred racehorse performance is largely influenced by a major quantitative trait locus at the myostatin (MSTN) gene which determines aptitude for certain race distances due to promoter region insertion mutation influencing functional phenotypes in skeletal muscle. To develop an vitro system experiments we established three novel equine muscle cell lines reflecting variation phenotype associated with MSTN genotype (CC/II, CT/IN and TT/NN SNP g.66493737C > T/SINE 227 bp polymorphism)....