A5070016179- Cancer Cells and Metastasis
- 3D Printing in Biomedical Research
- Cancer Genomics and Diagnostics
- Adenosine and Purinergic Signaling
- Prostate Cancer Treatment and Research
- Pharmacological Receptor Mechanisms and Effects
- Bone health and treatments
- Cancer Immunotherapy and Biomarkers
- Peptidase Inhibition and Analysis
- HER2/EGFR in Cancer Research
- Epigenetics and DNA Methylation
- MicroRNA in disease regulation
- Cellular Mechanics and Interactions
- Immune cells in cancer
- Pancreatitis Pathology and Treatment
- Extracellular vesicles in disease
- Pancreatic and Hepatic Oncology Research
- Cell Image Analysis Techniques
- Lung Cancer Treatments and Mutations
- Radiopharmaceutical Chemistry and Applications
- Nanoplatforms for cancer theranostics
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Advanced Breast Cancer Therapies
- Telomeres, Telomerase, and Senescence
- Mathematical Biology Tumor Growth
Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori
2016-2025
Istituti di Ricovero e Cura a Carattere Scientifico
2016-2025
Emory University
2023
Cancer Institute (WIA)
2023
Second Xiangya Hospital of Central South University
2023
Peking University
2023
Peking University First Hospital
2023
Central South University
2023
University of Bologna
2021
The potential of a spheroid tumor model composed cells in different proliferative and metabolic states for the development new anticancer strategies has been amply demonstrated. However, there is little or no information literature on problems reproducibility data originating from experiments using 3D models. Our analyses, carried out novel open source software capable performing an automatic image analysis colonies, showed that number morphology parameters affect response large spheroids to...
Abstract Prostate cancer represents the major cause of cancer-related death in men and patients frequently develop drug-resistance metastatic disease. Most studies focus on hormone-resistance mechanisms related to androgen receptor mutations or acquired property prostate cells over-activate signaling pathways. Tumor microenvironment plays a critical role progression. However, mechanism involving androgen/androgen associated fibroblasts consequences for progression still remains elusive. We...
Abstract Spheroids are three-dimensional cellular models with widespread basic and translational application across academia industry. However, methodological transparency guidelines for spheroid research have not yet been established. The MISpheroID Consortium developed a crowdsourcing knowledgebase that assembles the experimental parameters of 3,058 published spheroid-related experiments. Interrogation this identified heterogeneity in setup spheroids. Empirical evaluation interlaboratory...
Breast cancer is a leading cause of mortality worldwide. Pharmacological inhibitors cyclin-dependent kinases (CDK) 4 and 6 (CDK4/6i) inhibit breast growth by inducing senescent-like state. However, the long-term treatment efficacy remains limited development drug resistance, so clearance cells may extend durability treatment. we show here that while CDK4/6i-treated exhibit various senescence-associated phenotypes, they remain insensitive to common senolytic compounds. By searching for novel...
Tumor growth and metastatic spreading are heavily affected by the P2X7 receptor as well microvesicles exosomes release into tumor microenvironment. stimulation is known to trigger vesicular from immune central nervous system cells. However, role in delivery cells was never analyzed depth. Here we show that overexpressed patients malignant melanoma its expression closely correlates with reduced overall survival. Antagonism of cell-expressed inhibited vitro anchorage-independent migration vivo...
Glioblastoma (GBM) is the most lethal brain tumor in adults. Radiation, together with temozolomide standard treatment, but nevertheless, relapse occurs nearly all cases. Understanding mechanisms underlying radiation resistance may help to find more effective therapies. After ATP released into microenvironment where it binds and activates purinergic P2 receptors, mainly of P2X7 subtype. Two main splice variants, P2X7A P2X7B, are expressed cell types, they associate distinct biochemical...
Tumors are complex and heterogeneous diseases characterized by an intricate milieu dynamically in connection with surrounding distant tissues. In the last decades, great efforts have been made to develop novel preclinical models able recapitulate original features of tumors. However, development vitro functional realistic tumor organ is still utopic represents one major challenges reproduce architecture ecosystem. A strategy decrypt whole picture predict its behavior could be started from...
// Chiara Arienti 1 , Michele Zanoni Sara Pignatta Alberto Del Rio 2, 3 Silvia Carloni Michela Tebaldi Gianluca Tedaldi Anna Tesei Biosciences Laboratory, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, Meldola, Italy 2 Institute of Organic Synthesis and Photoreactivity (ISOF), National Research Council (CNR), Bologna, Innovamol Srls, Modena, Correspondence to: Arienti, e-mail: chiara.arienti@irst.emr.it Tesei, anna.tesei@irst.emr.it Keywords: trastuzumab...
Abstract Background Recent developments in abscopal effect strongly support the use of radiotherapy for treatment metastatic disease. However, deeper understanding molecular mechanisms underlying are required to best benefit a larger proportion patients with metastasis. Several groups including ours, reported involvement wild-type (wt) p53 radiation-induced effects, however very little is known on role wtp53 dependent mechanisms. Methods We investigated through vivo and vitro approaches how...
Ma and colleagues reported in their study on 12,004 elderly patients published Breast J. 2020, that adjuvant chemotherapy was not associated with overall survival. Given the toxicities systemic treatments, caution recommendation or omission of may be considered patient selection especially when comorbidities are present. We agree authors final conclusions but we want to highlight define therapy BC several factors need taken into account. One main issues is lack universal unique guidelines...
Abstract Tumor dissemination is a key event in tumor progression. During this event, main role played by circulating cells (CTCs), immune cells, and their interaction. How the system supports survival proliferation of CTCs not fully elucidated. In study we established an in-vitro co-culture consisting from same patient, which increased success rate establishment CTC-derived long-term cell cultures. system, characterized successful co-cultures signals they exchange with cancer including...
Rationale: Caloric restriction improves the efficacy of anti-cancer therapy.This effect is largely dependent on increase extracellular ATP concentration in tumor microenvironment (TME).Pathways for release triggered by nutrient deprivation are unknown.Methods: The (eATP) was vivo measured B16F10-inoculated C57Bl/6 mice with pmeLuc probe.Alternatively, pmeLuc-TG-mouse used.Caloric induced hydroxycitrate (HC).B16F10 melanoma cells or CT26 colon carcinoma were vitro exposed to serum starvation...
Breast cancer (BC) is the most common diagnosed and second leading cause of cancer-associated death in women, with triple negative (TNBC) subtype being characterized by poorest prognosis. New therapeutic targets are urgently needed to overcome high metastatic potential, aggressiveness poor survival these tumors. Trop2 transmembrane glycoprotein, acting as an intracellular calcium signal transducer, recently emerged a new potential target epithelial cancers, particular breast cancer.We...