A5053938706- Adenosine and Purinergic Signaling
- Adolescent and Pediatric Healthcare
- Neuroendocrine Tumor Research Advances
- Mast cells and histamine
- Gestational Trophoblastic Disease Studies
- Cancer Immunotherapy and Biomarkers
- Extracellular vesicles in disease
- Epigenetics and DNA Methylation
- Peptidase Inhibition and Analysis
- Nanoplatforms for cancer theranostics
- Immune Cell Function and Interaction
- Tryptophan and brain disorders
- Neuroblastoma Research and Treatments
- Pancreatic function and diabetes
- Cardiac Arrhythmias and Treatments
- Fibroblast Growth Factor Research
- Biochemical Analysis and Sensing Techniques
- Immune cells in cancer
- Click Chemistry and Applications
- Cancer, Stress, Anesthesia, and Immune Response
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- Neurological Complications and Syndromes
- Cardiac pacing and defibrillation studies
- Amino Acid Enzymes and Metabolism
University of Ferrara
2016-2025
Monash University
2022
Ludwig-Maximilians-Universität München
2022
Universidade de Santiago de Compostela
2022
University of Campania "Luigi Vanvitelli"
2018
Inserm
2012
Sorbonne Paris Cité
2012
Hôpital Européen Georges-Pompidou
2012
Institut Gustave Roussy
2012
Université Paris Cité
2012
The P2X7 receptor is an ATP-gated ion channel known for its cytotoxic activity. However, recent evidence suggests a role in cell proliferation. Here, we found that exhibits significant growth-promoting effects vivo. Human embryonic kidney cells expressing exhibited more tumorigenic and anaplastic phenotype than control vivo, the growth rate size of these tumors were significantly reduced by intratumoral injection inhibitor-oxidized ATP. accelerated P2X7-expressing was characterized increased...
In the tumor microenvironment (TME) ATP and its receptor P2X7 exert a pivotal influence on cancer growth tumor–host interactions. Here we analyzed different effect of genetic deficiency versus antagonism response against P2X7-expressing implanted tumors. We focused immune cell expression degrading enzymes CD39 CD73 in vivo measured TME's ATP. The infiltrate tumors growing null mice shows decrease CD8+ cells an increased number Tregs, overexpressing fitness markers OX40, PD-1, CD73. A similar...
P2X7 is a bifunctional receptor (P2X7R) for extracellular ATP that, depending on the level of activation, forms cation-selective channel or large conductance nonselective pore. The P2X7R has strong proapoptotic activity but can also support growth. Here, we describe mechanism involved in growth stimulation. Transfection increases resting mitochondrial potential (delta psi(mt)), basal Ca2+ ([Ca2+]mt), intracellular content, and confers ability to grow absence serum. These changes require full...
P2X7 is the largest member of P2X subfamily purinergic receptors. A typical feature carboxyl tail, which allows formation a large pore. Recently naturally occurring truncated splice variant, isoform B (P2X7B), has been identified. Here we show that P2X7B expression in HEK293 cells, cell type lacking endogenous receptors, mediated ATP-stimulated channel activity but not plasma membrane permeabilization, raised endoplasmic reticulum Ca2+ content, activated transcription factor NFATc1,...
Human leukocytes can express the P2X<sub>7</sub> purinergic receptor, an ionic channel gated by extracellular ATP, for which physiological role is only partially understood. Transfection of cDNA into lymphoid cells that lack this receptor sustains their proliferation in serum-free medium. Increased serum-starved transfectants abolished P2X<sub>7</sub>receptor blocker oxidized ATP or hydrolase apyrase. Both wild type and P2X<sub>7</sub>-transfected release large amounts culture These data...
The ATP receptor P2X7 (P2X7R or P2RX7) has a key role in inflammation and immunity, but its possible roles cancer are not firmly established. In the present study, we investigated effect of host genetic deletion P2X7R mouse on growth B16 melanoma CT26 colon carcinoma cells. Tumor size metastatic dissemination were assessed by vivo calliper luciferase luminescence emission measurements along with postmortem examination. P2X7R-deficient mice, tumor spreading accelerated strongly, compared...
Ability to adapt conditions of limited nutrient supply requires a reorganization the metabolic pathways balance energy generation and production biosynthetic intermediates. Several fast-growing cells overexpress P2X7 receptor (P2X7R) for extracellular ATP. A feature this is allow growth in absence serum. We show here that transfection P2X7R allows proliferation P2X7R-transfected HEK293 (HEK293-P2X7) not only serum but also low (4 mM) glucose, increases lactate output compared with...
P2X7 receptors are ATP-gated ion channels primarily expressed on antigen-presenting immune cells where they play a role in the acute inflammatory response. These couple not only to influx of cations, including calcium, but also rapid alterations cell morphology (membrane blebbing, phosphatidylserine exposure, microvesicle shedding). features resemble extranuclear events associated with end stages apoptosis death does occur if receptor activation is brief. Here we delineate two signaling...
We previously purified a new esterase from the thermoacidophilic eubacterium Bacillus acidocaldarius whose N-terminal sequence corresponds to an open reading frame (ORF3) reported show homology with mammalian hormone-sensitive lipase (HSL)-like group of esterase/lipase family. To compare biochemical properties this thermophilic enzyme those homologous mesophilic and psychrophilic members HSL group, overexpression system in Escherichia coli was established. The protein, expressed soluble...
The P2X(7) plasma membrane receptor is an intriguing molecule that endowed with the ability to kill cells, as well activate many responses and even stimulate proliferation. Here, authors give overview on multiplicity complexity of P2X(7)-mediated responses, discussing recent information this receptor. Particular attention has been paid early late signs apoptosis necrosis linked activation emerging field function in carcinogenesis.
The P2X(7) receptor is known for the cytotoxic activity because of its ability to cause opening non-selective pores in plasma membrane and activate apoptotic caspases. A key factor P2X(7)-dependent cytotoxicity massive intracellular Ca(2+) increase triggered by activation. Here we show that transfection increased endoplasmic reticulum accumulate, store, release Ca(2+). This caused a larger agonist-stimulated cytosol mitochondrial transfectants than mock transfected cells. survived even...
The P2X7 receptor (P2X7R) is attracting increasing attention for its involvement in cancer. Several recent studies have shown a crucial role of P2X7R tumour cell growth, angiogenesis and invasiveness. In this study, we investigated the two known human functional splice variants, full length P2X7RA truncated P2X7RB, osteosarcoma growth. Immunohistochemical analysis tissue array osteosarcomas showed that forty-four, total fifty-four tumours (81.4%), stained positive both B, thirty-one (57.4%)...
Chemotherapy-induced immunogenic cell death can favor dendritic cross-priming of tumor-associated antigens for T activation thanks to the release damage-associated molecular patterns, including ATP. Here, we tested hypothesis that in acute myeloid leukemia, ATP release, along with its well-known immune stimulatory effect, may also contribute generation an suppressive microenvironment. In a cohort leukemia patients, undergoing combined daunorubicin and cytarabine chemotherapy, population...
Abstract Previous data from our laboratory show that expression of the P2X7 receptor (P2X7R) is needed for amyloid β (Aβ)-stimulated microglia activation and IL-1β release in vitro vivo . We also showed Aβ-dependent stimulation inhibited by dihydropyridine nimodipine at an intracellular site distal to P2X7R. In present study, we used N13 cell line mouse primary wt P2rx7 -deleted mice test effect on (Aβ)-dependent NLRP3 inflammasome function, mitochondrial energy metabolism. Our Aβ causes...