A5088779262- Neuroblastoma Research and Treatments
- Cancer, Hypoxia, and Metabolism
- Adenosine and Purinergic Signaling
- Cancer Treatment and Pharmacology
- Calcium signaling and nucleotide metabolism
- Metabolism, Diabetes, and Cancer
- Cancer Immunotherapy and Biomarkers
- Retinoids in leukemia and cellular processes
- Cancer therapeutics and mechanisms
- Lung Cancer Research Studies
- Immune cells in cancer
- Cell death mechanisms and regulation
- Chronic Lymphocytic Leukemia Research
- Parkinson's Disease Mechanisms and Treatments
- CAR-T cell therapy research
- Estrogen and related hormone effects
- Pancreatic function and diabetes
- Nanoplatforms for cancer theranostics
- Immunodeficiency and Autoimmune Disorders
- Virus-based gene therapy research
- Glioma Diagnosis and Treatment
- RNA Interference and Gene Delivery
- Nanoparticle-Based Drug Delivery
- Neuroscience and Neuropharmacology Research
- Amyotrophic Lateral Sclerosis Research
Ospedale Policlinico San Martino
2015-2024
University of Genoa
2002-2022
Istituti di Ricovero e Cura a Carattere Scientifico
2013-2017
Alleanza Contro il Cancro
2011-2017
National Cancer Research Institute
2003-2013
ANT Foundation Italy Onlus
2010-2011
National Cancer Institute
2010
University of Bari Aldo Moro
2010
Short-term starvation (or fasting) protects normal cells, mice, and potentially humans from the harmful side effects of a variety chemotherapy drugs. Here, we show that treatment with conditions sensitized yeast cells (Saccharomyces cerevisiae) expressing oncogene-like RAS2(val19) to oxidative stress 15 17 mammalian cancer cell lines chemotherapeutic agents. Cycles were as effective agents in delaying progression different tumors increased effectiveness these drugs against melanoma, glioma,...
Tumor chemoresistance is associated with high aerobic glycolysis rates and reduced oxidative phosphorylation, a phenomenon called "Warburg effect" whose reversal could impair the ability of wide range cancer cells to survive in presence or absence chemotherapy. In previous studies, Short-term-starvation (STS) was shown protect normal organs but sensitize different cell types chemotherapy mechanisms responsible for these effects are poorly understood. We tested cytotoxicity Oxaliplatin (OXP)...
The ATP receptor P2X7 (P2X7R or P2RX7) has a key role in inflammation and immunity, but its possible roles cancer are not firmly established. In the present study, we investigated effect of host genetic deletion P2X7R mouse on growth B16 melanoma CT26 colon carcinoma cells. Tumor size metastatic dissemination were assessed by vivo calliper luciferase luminescence emission measurements along with postmortem examination. P2X7R-deficient mice, tumor spreading accelerated strongly, compared...
Emerging evidence suggests that metformin, a widely used anti-diabetic drug, may be useful in the prevention and treatment of different cancers. In present study, we demonstrate metformin directly inhibits enzymatic function hexokinase (HK) I II cell line triple-negative breast cancer (MDA-MB-231). The inhibition is selective for these isoforms, as documented by experiments with purified HK well lysates. Measurements (18)F-fluoro-deoxyglycose uptake document it dose- time-dependent powerful...
Tumor microenvironment of solid tumors is characterized by a strikingly high concentration adenosine and ATP. Physiological significance this biochemical feature unknown, but it has been suggested that may affect infiltrating immune cell responses tumor progression. There increasing awareness many the effects extracellular ATP on inflammatory cells are mediated P2X7 receptor (P2X7R). Aim study was to investigate whether: (i) component neuroblastoma (NB) microenvironment, (ii) myeloid-derived...
GD2-redirected chimeric antigen receptor (CAR) T lymphocytes represent a promising therapeutic option for immunotherapy of neuroblastoma (NB). However, despite the encouraging effects observed in some hematological malignancies, clinical results CAR cell solid tumors are still modest. Tumor driven neo-angiogenesis supports an immunosuppressive microenvironment that influences treatment responses and is amenable to targeting with antiangiogenic drugs. The latter agents promote lymphocyte...
Abstract Cancer metabolism is characterized by an accelerated glycolytic rate facing reduced activity of oxidative phosphorylation. This “Warburg effect” represents a standard to diagnose and monitor tumor aggressiveness with 18 F-fluorodeoxyglucose whose uptake currently regarded as accurate index total glucose consumption. Studying cancer metabolic response respiratory chain inhibition metformin, we repeatedly observed reduction tracer marked increase in puzzling discordance brought us...
Aberrant histone deacetylase (HDAC) activity is frequent in human leukemias. However, while classical, NAD+-independent HDACs are an established therapeutic target, the relevance of NAD+-dependent (sirtuins) leukemia treatment remains unclear. Here, we assessed antileukemic sirtuin inhibitors and NAD+-lowering drug FK866, alone combination with traditional HDAC inhibitors. Primary cells, cell lines, healthy leukocytes hematopoietic progenitors were treated (sirtinol, cambinol, EX527) or...
The present translational study aimed to verify whether serial 18F-FDG PET/CT predicts doxorubicin cardiotoxicity. Methods: Fifteen athymic mice were treated intravenously with saline (n = 5) or 5 7.5 mg of per kilogram each) and underwent dynamic small-animal PET beforehand afterward estimate left ventricular (LV) metabolic rate glucose (MRGlu). Thereafter, we retrospectively identified 69 patients who had been successfully a regimen doxorubicin, bleomycin, vinblastine, dacarbazine for...
// Giovanna Sociali 1, * , Lizzia Raffaghello 2, Mirko Magnone 1 Federica Zamporlini 3 Laura Emionite 4 Sturla Bianchi 2 Tiziana Vigliarolo Aimable Nahimana 5 Alessio Nencioni 6, 7 Nadia Raffaelli Santina Bruzzone Department of Experimental Medicine, Section Biochemistry, and CEBR, University Genova, 16132 Italy Laboratorio di Oncologia Istituto G. Gaslini, 16147 Agricultural, Food Environmental Sciences, Polytechnic Marche, 60131 Ancona, Animal Facility, IRCCS AOU San Martino - IST...
Abstract Long noncoding RNAs (lncRNAs) are emerging as regulators of fundamental biological processes. Here we report on the characterization an intergenic lncRNA expressed in epithelial tissues which termed EPR (Epithelial cell Program Regulator). is rapidly downregulated by TGF-β and its sustained expression largely reshapes transcriptome, favors acquisition traits, reduces proliferation cultured mammary gland cells well animal model orthotopic transplantation. generates a small peptide...
2-Cis,4-trans-abscisic acid (ABA) is a plant hormone that present also in animals. Several lines of evidence suggest ABA contributes to the regulation glycemia mammals: nanomolar stimulates insulin release from β-pancreatic cells and glucose transporter-4-mediated uptake by myoblasts adipocytes vitro; plasma increases normal human subjects, but not diabetic patients, after load for an oral tolerance test (OGTT). The presence fruits prompted exploration bioavailability dietary effect ABA-rich...
Treatment of neuroblastoma is successful in less than half patients with high-risk disease. The antitumor activity a water soluble pegylated SN38 drug conjugate, EZN-2208, was compared CPT-11 (a prodrug for SN38) preclinical models human neuroblastoma.The vitro cytotoxicity EZN-2208 tested by counting trypan blue dye- and Annexin V-positive cells, whereas its therapeutic efficacy evaluated, terms survival, antiangiogenic activities, s.c. luciferase-transfected, pseudometastatic, orthotopic...
The anaplastic lymphoma kinase (ALK) is a tyrosine receptor that involved in the pathogenesis of different types human cancers, including neuroblastoma (NB). In NB, ALK overexpression, or point mutations, are associated with poor prognosis and advanced stage disease. Inhibition activity by small-molecule inhibitors lung cancers carrying translocations has shown therapeutic potential. However, secondary mutations may occur that, generate tumor resistance to inhibitors. To overcome we adopted...
In the course of metformin treatment, staging abdominal cancer lesions with (18)F-FDG PET images is often hindered by presence a high bowel radioactivity. The present study aimed to verify mechanism underlying this phenomenon.Fifty-three mice were submitted dynamic acquisitions kinetics under fasting conditions. Three small-animal scans obtained over 4-mo period. animals subdivided into 4 groups according following administration protocol: group 1, untreated (n = 15); 2, exposed treatment...
Curcumin has been reported to inhibit inflammation, tumor growth, angiogenesis and metastasis by decreasing cell growth inducing apoptosis mainly through the inhibition of nuclear factor kappa-B (NFκB), a master regulator inflammation. Recent reports also indicate potential metabolic effects polyphenol, therefore we analyzed whether how it affects energy metabolism cells. We show that curcumin (10 µM) inhibits activity ATP synthase in isolated mitochondrial membranes leading dramatic drop...
Abstract Emerging evidence demonstrates that targeting energy metabolism is a promising strategy to fight cancer. Here we show combining metformin and short-term starvation markedly impairs growth of colon breast The impairment in glycolytic flux caused by enhanced through its interference with hexokinase II activity, as documented measurement 18F-fluorodeoxyglycose uptake. Oxidative phosphorylation additively compromised combined treatment: virtually abolishes Complex I function; determines...
Neuroblastoma (NB) represents the most frequent and aggressive form of extracranial solid tumor infants. Nucleolin (NCL) is a protein overexpressed partially localized on cell surface cells adult cancers. Little known about NCL pediatric tumors nothing reported NB.NB lines, Schwannian stroma-poor NB bone marrow (BM)-infiltrating were evaluated for expression by Flow Cytometry, Imaging Cytometry Immunohistochemistry analyses. The cytotoxic activity doxorubicin (DXR)-loaded nanocarriers...
RNA interference molecules have some advantages as cancer therapeutics, including a proved efficacy on both wild-type (WT) and mutated transcripts an extremely high sequence-specificity. The most significant hurdle to be overcome if exogenous small interfering RNAs (siRNA) is used therapeutically the specific, effective, nontoxic delivery of siRNA its intracellular site action. At present, human applications are confined almost exclusively targets within liver, where systems naturally...
Abstract Background The DLX gene family encodes for homeobox transcription factors involved in the control of morphogenesis and tissue homeostasis. Their expression can be regulated by Endothelin1 (ET1), a peptide associated with breast cancer invasive phenotype. Deregulation was found human solid tumors hematologic malignancies. In particular, DLX4 overexpression represents possible prognostic marker ovarian cancer. We have investigated role genes progression. Methods MDA-MB-231 carcinoma...
Abstract Activated leukocyte cell adhesion molecule (ALCAM) is involved in cell–cell interactions cancer. Shedding of its ectodomain by the metalloprotease ADAM17/TACE generates a soluble form (sALCAM). Here, we show that serum sALCAM levels were significantly higher epithelial ovarian cancer (EOC) ( p < 0.005) than controls. The performance as classifier, tested receiver operating characteristic curve, resulted an area under curve (AUC) 0.8067. Serum showed direct correlation with...