A5054820276- Cancer Immunotherapy and Biomarkers
- Esophageal Cancer Research and Treatment
- Cancer Genomics and Diagnostics
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Cancer-related molecular mechanisms research
- Lymphoma Diagnosis and Treatment
- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Acute Myeloid Leukemia Research
- Genomics and Phylogenetic Studies
- Immunotherapy and Immune Responses
- Lung Cancer Treatments and Mutations
- Genetics, Bioinformatics, and Biomedical Research
- Cancer-related gene regulation
- RNA and protein synthesis mechanisms
- MicroRNA in disease regulation
- Gene expression and cancer classification
- Circular RNAs in diseases
- Genomics and Rare Diseases
- Hematopoietic Stem Cell Transplantation
- CRISPR and Genetic Engineering
- Neuroendocrine Tumor Research Advances
- Histone Deacetylase Inhibitors Research
- Advanced biosensing and bioanalysis techniques
Istituti di Ricovero e Cura a Carattere Scientifico
2016-2024
Istituto di Ricovero e Cura a Carattere Scientifico San Raffaele
2016-2024
Vita-Salute San Raffaele University
2015-2023
IRCCS Ospedale San Raffaele
2014-2023
Northwestern University
2018
European Institute of Oncology
2009-2015
Center for Genomic Science
2012-2014
Italian Institute of Technology
2012-2014
San Raffaele University of Rome
2014
University of Reading
2014
The BioMart Community Portal (www.biomart.org) is a community-driven effort to provide unified interface biomedical databases that are distributed worldwide. portal provides access numerous database projects supported by 30 scientific organizations. It includes over 800 different biological datasets spanning genomics, proteomics, model organisms, cancer data, ontology information and more. All resources available through the independently administered funded their host data federation...
Significance Long noncoding RNAs (lncRNAs) provide new layers of complexity to gene expression control. We report on the functional consequences interaction between ssRNA-binding protein K homology-type splicing regulatory (KSRP) with H19 lncRNA (H19) in multipotent C2C12 cells able differentiate culture toward myotubes response activation cell signaling pathways, including AKT. KSRP and interact exclusively undifferentiated cells, this favors KSRP’s ability promyogenic transcript myogenin...
Abstract Long noncoding RNAs (lncRNAs) are emerging as regulators of fundamental biological processes. Here we report on the characterization an intergenic lncRNA expressed in epithelial tissues which termed EPR (Epithelial cell Program Regulator). is rapidly downregulated by TGF-β and its sustained expression largely reshapes transcriptome, favors acquisition traits, reduces proliferation cultured mammary gland cells well animal model orthotopic transplantation. generates a small peptide...
Transcription factor (TF)–induced reprogramming of somatic cells into induced pluripotent stem (iPSC) is associated with genome-wide changes in chromatin modifications. Polycomb-mediated histone H3 lysine-27 trimethylation (H3K27me3) has been proposed as a defining mark that distinguishes the from iPSC epigenome. Here, we dissected functional role H3K27me3 TF–induced through inactivation H3K27 methylase EZH2 at onset reprogramming. Our results demonstrate surprisingly establishment proceeds...
α-Synuclein is thought to regulate neurotransmitter release through multiple interactions with presynaptic proteins, cytoskeletal elements, ion channels, and synaptic vesicles membrane. abundant in the compartment, its from neurons glia has been described as responsible for spreading of α-synuclein-derived pathology. α-Synuclein-dependent dysregulation might occur via action on surface-exposed calcium channels. Here, we provide electrophysiological biochemical evidence show that α-synuclein,...
Epithelial-to-mesenchymal transition (EMT) confers several traits to cancer cells that are required for malignant progression. Here, we report miR-27b-3p-mediated silencing of the single-strand RNA binding protein KHSRP is transforming growth factor β (TGF-β)-induced EMT in mammary gland cells. Sustained expression limits TGF-β-dependent induction factors and cell migration, whereas its knockdown untreated mimics TGF-β-induced EMT. Genome-wide sequencing analyses revealed controls (1) levels...
The limited clinical response observed in high-grade serous ovarian cancer (HG-SOC) with high frequency of TP53 mutations (mutp53) might be related to mutp53-driven oncogenic pathway network. Here we show that β-arrestin1 (β-arr1), interacts YAP, triggering its cytoplasmic-nuclear shuttling. This interaction allows β-arr1 recruit mutp53 the YAP-TEAD transcriptional complex upon activation endothelin-1 receptors (ET-1R) patient-derived HG-SOC cells and cell lines bearing mutp53. In parallel,...
Epigenetic alterations in the pattern of DNA and histone modifications play a crucial role cancer development. Analysis patient samples, however, is hampered by technical limitations study chromatin structure from pathology archives that usually consist heavily fixed, paraffin-embedded material. Here, we present methodology [pathology tissue–ChIP (PAT-ChIP)] to extract immunoprecipitate samples up several years old. In pairwise comparison with canonical ChIP, PAT-ChIP showed high...
The cancer-associated loss of microRNA (miRNA) expression leads to a proliferative advantage and aggressive behavior through largely unknown mechanisms. Here, we exploit model system that recapitulates physiological terminal differentiation its reversal upon oncogene analyze coordinated mRNA/miRNA responses. cell cycle reentry myotubes, forced by the E1A oncogene, was associated with pattern modulation reciprocal induced during myoblasts into myotubes. E1A-induced mRNA response...
Current treatment for patients with locally advanced esophageal adenocarcinoma (EAC) is neoadjuvant chemotherapy (nCT), alone or combined radiotherapy, before surgery. However, fewer than 30% of treated show a pathologic complete response to nCT, which correlates increased 5-year survival compared nonresponders. Understanding the mechanisms nCT pivotal better stratify and inform more efficacious therapies. Here, we investigated immune involved in by multidimensional profiling pretreatment...
MicroRNAs (miRNAs) are essential regulators of development, physiology, and evolution, their biogenesis is strictly controlled at multiple levels. Regulatory proteins, such as KSRP, modulate rates timing enzymatic reactions responsible for maturation select miRNAs from primary transcripts in response to specific stimuli. Here, we show that KSRP silencing mesenchymal C2C12 cells produces a change the transcriptome largely overlapping induced by bone morphogenetic protein 2 (BMP2) signaling...
Abstract Long non-coding RNAs (lncRNAs) can affect multiple layers of gene expression to control crucial cellular functions. We have previously demonstrated that the lncRNA EPR, by controlling at different levels, affects cell proliferation and migration in cultured mammary gland cells impairs breast tumor formation an orthotopic transplant model mice. Here, we used ChIRP-Seq identify EPR binding sites on chromatin NMuMG overexpressing identified its trans genome. Then, with purpose relating...
The long non-coding RNA EPR is expressed in epithelial tissues, binds to chromatin and controls distinct biological activities mouse mammary gland cells. Because of its high expression the intestine, this study we have generated a colon-specific conditional targeted deletion (EPR cKO) evaluate vivo functions mice. cKO mice display epithelium hyperproliferation, impaired mucus production secretion, as well inflammatory infiltration proximal portion large intestine. sequencing analysis reveals...
EPR is a long non-coding RNA (lncRNA) that controls cell proliferation in mammary gland cells by regulating gene transcription. Here, we report on Mettl7a1 as direct target of EPR. We show induces transcription rewiring three-dimensional chromatin interactions at the locus. Our data indicate METTL7A1 contributes to EPR-dependent inhibition TGF-β signaling. absent tumorigenic murine and its human ortholog (METTL7A) downregulated breast cancers. Importantly, re-expression 4T1 attenuates their...
Classification of lung carcinoids into typical and atypical is a diagnostic challenge since no immunohistochemical tools are available to support pathologists in distinguishing between the two subtypes. A differential diagnosis essential for clinicians correctly discuss therapy, prognosis follow-up with patients. Indeed, distinction subtypes on biopsies/cytological specimens still unfeasible sometimes limited also after radical surgeries. By comparing gene expression profile (TC) (AC), we...
Vorinostat (suberoylanilide hydroxamic acid; SAHA) is a histone deacetylase inhibitor (HDACi) approved in the clinics for treatment of T-cell lymphoma and with potential to be effective also breast cancer. We investigated responsiveness SAHA human primary tumors cancer cell lines.We observed differential response drug both lines. Gene expression analysis lines revealed that genes involved adhesion redox pathways, especially glutathione metabolism, were differentially expressed resistant...