A5071288353- CAR-T cell therapy research
- Virus-based gene therapy research
- Immunotherapy and Immune Responses
- Cancer Research and Treatments
- Immune Cell Function and Interaction
- Chronic Lymphocytic Leukemia Research
- Cancer Immunotherapy and Biomarkers
- Viral Infectious Diseases and Gene Expression in Insects
- T-cell and B-cell Immunology
- Cancer Genomics and Diagnostics
- Bladder and Urothelial Cancer Treatments
- Phagocytosis and Immune Regulation
- Gut microbiota and health
- Gene expression and cancer classification
- RNA Interference and Gene Delivery
- MicroRNA in disease regulation
- Bioinformatics and Genomic Networks
- RNA Research and Splicing
- Metabolism, Diabetes, and Cancer
- Acute Myeloid Leukemia Research
- PI3K/AKT/mTOR signaling in cancer
- IL-33, ST2, and ILC Pathways
- Epigenetics and DNA Methylation
- CRISPR and Genetic Engineering
- Histone Deacetylase Inhibitors Research
Istituti di Ricovero e Cura a Carattere Scientifico
2016-2024
Istituto di Ricovero e Cura a Carattere Scientifico San Raffaele
2016-2024
Vita-Salute San Raffaele University
2015-2024
IRCCS Ospedale San Raffaele
2012-2024
Center for Translational Research in Neuroimaging and Data Science
2019
Okayama University Hospital
2016
Kyoto University
2016
Tokai University
2016
San Raffaele University of Rome
2014-2015
Azienda Sanitaria Ospedaliera S.Croce e Carle Cuneo
2009
The BioMart Community Portal (www.biomart.org) is a community-driven effort to provide unified interface biomedical databases that are distributed worldwide. portal provides access numerous database projects supported by 30 scientific organizations. It includes over 800 different biological datasets spanning genomics, proteomics, model organisms, cancer data, ontology information and more. All resources available through the independently administered funded their host data federation...
Abstract Microenvironmental signals strongly influence chronic lymphocytic leukemia (CLL) cells through the activation of distinct membrane receptors, such as B-cell and inflammatory Toll-like receptors (TLRs). Inflammatory pathways downstream these lead to NF-κB activation, thus protecting leukemic from apoptosis. Dimethyl fumarate (DMF) is an anti-inflammatory immunoregulatory drug used treat patients with multiple sclerosis psoriasis in which it blocks aberrant impacts NRF2 antioxidant...
The role of monocytes/macrophages in the development and progression chronic lymphocytic leukemia (CLL) is poorly understood. Transcriptomic analyses show that leukemic cells cross talk during CLL progression. Macrophage depletion impairs engraftment, drastically reduces growth, favorably impacts mouse survival. Targeting macrophages by either CSF1R signaling blockade or clodrolip-mediated cell killing has marked inhibitory effects on established also. induces death mainly via TNF pathway...
Neoadjuvant pembrolizumab has been shown to be a valid treatment for patients affected by muscle-invasive bladder cancer (MIBC), as demonstrated in the PURE-01 clinical trial (NCT02736266). Among tumor-extrinsic factors influencing immunotherapy efficacy, extensive data highlighted that microbiome is central player immune-mediated anticancer activity. This report aimed investigate composition and role of stool enrolled trial. An orthotopic animal model (MB49-Luc) was used support some...
<h3>Background</h3> The thymic stromal lymphopoietin (TSLP), a key cytokine for development of Th2 immunity, is produced by cancer associated fibroblasts (CAFs) in pancreatic where predominant tumor infiltrating over Th1 cells correlates with reduced patients' survival. Which and molecules are mostly relevant driving TSLP secretion CAFs not defined. <h3>Methods</h3> We performed vitro, vivo ex-vivo analyses. For vitro studies we used cell lines, primary cultures, THP1 cells. was as read-out...
Abstract Clinical application of lentiviral vector (LV)‐based hematopoietic stem and progenitor cells (HSPC) gene therapy is rapidly becoming a reality. Nevertheless, LV‐mediated signaling its potential functional consequences on HSPC biology remain poorly understood. We unravel here remarkably limited impact LV the transcriptional landscape. escaped innate immune sensing that instead led to robust IFN responses upon transduction with gamma‐retroviral vector. However, reverse‐transcribed DNA...
Significance CD1d-restricted invariant natural killer T (iNKT) cells are innate-like lymphocytes that play fundamental roles in cancer, autoimmunity, and infections. iNKT acquire effector functions already the thymus, because of a distinct developmentally regulated genetic program is critically controlled by miRNAs. Our study unveils unexpected requirement for miRNA-dependent fine-tuning TGF-β signaling control cell development functional differentiation. The targeting lineage-specific...
The epigenetic silencing of exogenous transcriptional units integrated into the genome represents a critical problem both for long-term gene therapy efficacy and eradication latent viral infections. We report here that limitation essential amino acids, such as methionine cysteine, causes selective up-regulation transgene expression in mammalian cells. Prolonged acid deprivation led to significant reversible increase levels stably transgenes transcribed by means or human promoters HeLa This...
Autosomal dominant tubulointerstitial kidney disease (ADTKD) is an inherited disorder that causes progressive damage and renal failure. Mutations in the UMOD gene, encoding uromodulin, lead to ADTKD-UMOD related. Uromodulin a GPI-anchored protein exclusively produced by epithelial cells of thick ascending limb Henle's loop. It released tubular lumen after proteolytic cleavage represents most abundant human urine physiological condition. We previously generated characterized transgenic mouse...
Donor-derived allogeneic T cells evoke potent graft versus tumor (GVT) effects likely due to the simultaneous recognition of tumor-specific and host-restricted minor histocompatibility (H) antigens. Here we investigated whether such could be reproduced in autologous settings by TCR gene-engineered lymphocytes. We report that redirected either a broadly expressed Y-encoded H antigen or tumor-associated antigen, although poorly effective if individually transferred, when simultaneously...
Myotonic dystrophy type 2 (DM2) is a genetic, autosomal dominant disease due to expansion of tetraplet (CCTG) repetitions in the first intron ZNF9/CNBP gene. DM2 multisystemic disorder affecting skeletal muscle, heart, eye and endocrine system. According proposed pathological mechanism, expanded tetraplets have an RNA toxic effect, disrupting splicing many mRNAs. Thus, identification aberrantly spliced transcripts instrumental for our understanding molecular mechanisms underpinning disease....
Control of systemic iron homeostasis is interconnected with the inflammatory response through key regulator, antimicrobial peptide hepcidin. We have previously shown that mice deficiency anemia (IDA)-low hepcidin show a pro-inflammatory blunted in deficient-high Tmprss6 KO mice. The transcriptional associated chronic overexpression due to genetic inactivation unknown. By using whole genome transcription profiling liver and analysis spleen immune-related genes we identified several functional...
Abstract Lipid and cholesterol metabolism play a crucial role in tumor cell behavior shaping the microenvironment. In particular, enzymatic non-enzymatic metabolism, derived metabolites control dendritic (DC) functions, ultimately impacting antigen presentation within outside mass, dampening immunity immunotherapeutic attempts. The mechanisms accounting for such events remain largely to be defined. Here we perturbed (oxy)sterol genetically pharmacologically analyzed lipidome landscape...
Chronic lymphocytic leukemia (CLL) co-evolves with its own microenvironment where inflammatory stimuli including toll-like receptors (TLR) signaling can protect CLL cells from spontaneous and drug-induced apoptosis by upregulating IκBζ, an atypical co-transcription factor. To dissect IκBζ-centered pathways, we performed a gene expression profile of primary leukemic expressing either high or low levels IκBζ after stimulation, highlighting that is not only but it may control metabolic rewiring...
In a variety of species, reduced food intake, and in particular protein or amino acid (AA) restriction, extends lifespan healthspan. However, the underlying epigenetic and/or transcriptional mechanisms are largely unknown, dissection specific pathways cultured cells may contribute to filling this gap. We have previously shown that, mammalian cells, deprivation essential AAs (methionine/cysteine tyrosine) leads reactivation integrated silenced transgenes, including plasmid retroviral vectors...
The choroid plexus (ChP) is a secretory tissue that produces cerebrospinal fluid (CSF) secreted into the ventricular system. It monolayer of secretory, multiciliated epithelial cells derived from neuroepithelial progenitors and overlying stroma mesenchymal mesodermal origin. Zfp423, which encodes Kruppel-type zinc-finger transcription factor essential for cerebellar development mutated in rare cases vermis hypoplasia/Joubert syndrome other ciliopathies, expressed hindbrain roof plate, IV...
Current palliative chemotherapy (CT) regimens achieve clinical benefits in less than 50% of patients treated for metastatic gastric cancers, and long-term survivals are anecdotical. Genetic polymorphisms differences at the level transcription genes involved biological processes drug metabolism, DNA repair resistance can explain observed individual response to drugs, survival different susceptibility toxic effects CT. The possibility classify on basis genetic signatures could help choosing CT...