A5060878254- RNA Research and Splicing
- RNA modifications and cancer
- MicroRNA in disease regulation
- RNA and protein synthesis mechanisms
- Cancer-related molecular mechanisms research
- Developmental Biology and Gene Regulation
- Cancer-related gene regulation
- Metabolism, Diabetes, and Cancer
- Animal Genetics and Reproduction
- RNA regulation and disease
- Congenital heart defects research
- Ubiquitin and proteasome pathways
- Pancreatic function and diabetes
- Wnt/β-catenin signaling in development and cancer
- Immune Cell Function and Interaction
- Neurobiology and Insect Physiology Research
- Nuclear Receptors and Signaling
- 14-3-3 protein interactions
- PI3K/AKT/mTOR signaling in cancer
- Protein Kinase Regulation and GTPase Signaling
- Lipid metabolism and biosynthesis
- Nuclear Structure and Function
- RNA Interference and Gene Delivery
- Cell Adhesion Molecules Research
- Retinoids in leukemia and cellular processes
Ospedale Policlinico San Martino
2013-2023
Alleanza Contro il Cancro
2002-2017
Istituti di Ricovero e Cura a Carattere Scientifico
2012-2014
The Francis Crick Institute
2012
Ceinge Biotecnologie Avanzate (Italy)
2011
Howard Hughes Medical Institute
1999-2010
University of California, San Diego
1999-2010
Unidades Centrales Científico-Técnicas
2010
Consejo Superior de Investigaciones Científicas
2010
Oregon State University
2002
The importance of post-transcriptional mechanisms for the regulation homoeostasis immune system and response to challenge by microorganisms is becoming increasingly appreciated. We investigated contribution microRNAs (miRNAs) macrophage activation induced lipopolysaccharide (LPS). first observed that Dicer knockout in bone marrow-derived macrophages (BMDMs) increases LPS-induced expression some inflammation mediators. miRNA microarray analysis BMDMs revealed LPS significantly induces a...
In eukaryotes, RNA-binding proteins that contain multiple K homology (KH) domains play a key role in coordinating the different steps of RNA synthesis, metabolism and localization. Understanding how KH modules participate recognition targets is necessary to dissect way these operate. We have designed mutant with impaired capability for general use exploring individual combinatorial functional targets. A double mutation hallmark GxxG loop (GxxG-to-GDDG) impairs nucleic acid binding without...
Significance Long noncoding RNAs (lncRNAs) provide new layers of complexity to gene expression control. We report on the functional consequences interaction between ssRNA-binding protein K homology-type splicing regulatory (KSRP) with H19 lncRNA (H19) in multipotent C2C12 cells able differentiate culture toward myotubes response activation cell signaling pathways, including AKT. KSRP and interact exclusively undifferentiated cells, this favors KSRP’s ability promyogenic transcript myogenin...
The expression pattern of Otx2, a homeobox-containing gene, was analyzed from the beginning eye morphogenesis until neural retina differentiation in chick embryos. Early on, Otx2 diffuse throughout optic vesicles but became restricted to their dorsal part when contacted surface ectoderm. As cup forms, expressed only outer layer, which gives rise pigment epithelium. This early complementary that PAX2, localizes ventral half developing and stalk. always observed epithelium at all stages...
ABSTRACT Otx1 and Otx2, two murine homologs of the Drosophila orthodenticle (otd) gene, contribute to brain morphogenesis. In particular null mice are viable show spontaneous epileptic seizures abnormalities affecting dorsal telencephalic cortex. Otx2 die early in development fail specification rostral neuroectoderm proper gastrulation. order determine whether Otx1−/− Otx2−/− highly divergent phenotypes reflect differences temporal expression or biochemical activity OTX1 OTX2 proteins, Otx2-...
Pitx2 is a bicoid-related homeodomain factor that required for effective cell type-specific proliferation directly activating specific growth-regulating gene cyclin D2 . Here, we report Pitx2, in response to the Wnt/β-catenin pathway and growth signals, also can regulate c -Myc D1. Investigation of molecular mechanisms Pitx2-dependent proliferation, these cases, further supports nuclear role β-catenin preventing histone deacetylase 1-dependent inhibitory functions several DNA-binding...
Beta-catenin plays an essential role in several biological events including cell fate determination, proliferation, and transformation. Here we report that beta-catenin is encoded by a labile transcript whose half-life prolonged Wnt phosphatidylinositol 3-kinase-AKT signaling. AKT phosphorylates the mRNA decay-promoting factor KSRP at unique serine residue, induces its association with multifunctional protein 14-3-3, prevents interaction exoribonucleolytic complex exosome. This impairs...
Abstract Long noncoding RNAs (lncRNAs) are emerging as regulators of fundamental biological processes. Here we report on the characterization an intergenic lncRNA expressed in epithelial tissues which termed EPR (Epithelial cell Program Regulator). is rapidly downregulated by TGF-β and its sustained expression largely reshapes transcriptome, favors acquisition traits, reduces proliferation cultured mammary gland cells well animal model orthotopic transplantation. generates a small peptide...
White adipose tissue (WAT) releases fatty acids from stored triacylglycerol for an energy source. Here, we report that targeted deletion of KH-type splicing regulatory protein (KSRP), RNA-binding regulates gene expression at multiple levels, enhances lipolysis in epididymal WAT (eWAT) because the upregulation genes promoting lipolytic activity. Expression microRNA 145 (miR-145) is decreased impaired primary miR-145 processing Ksrp(-/-) eWAT. We show directly targets and represses Foxo1...
Epithelial-to-mesenchymal transition (EMT) confers several traits to cancer cells that are required for malignant progression. Here, we report miR-27b-3p-mediated silencing of the single-strand RNA binding protein KHSRP is transforming growth factor β (TGF-β)-induced EMT in mammary gland cells. Sustained expression limits TGF-β-dependent induction factors and cell migration, whereas its knockdown untreated mimics TGF-β-induced EMT. Genome-wide sequencing analyses revealed controls (1) levels...
Brown adipose tissue oxidizes chemical energy for heat generation and expenditure. Promoting brown-like transformation in white (WAT) is a promising strategy combating obesity. Here, we find that targeted deletion of KH-type splicing regulatory protein (KSRP), an RNA-binding regulates gene expression at multiple levels, causes reduction body adiposity. The brown fat-selective genes increased subcutaneous/inguinal WAT (iWAT) Ksrp(-/-) mice because the elevated PR domain containing 16...