A5059105159- Congenital heart defects research
- Developmental Biology and Gene Regulation
- Animal Genetics and Reproduction
- Muscle Physiology and Disorders
- RNA Research and Splicing
- Pluripotent Stem Cells Research
- Signaling Pathways in Disease
- Hops Chemistry and Applications
- Nuclear Receptors and Signaling
- dental development and anomalies
- Cancer-related gene regulation
- Bone and Dental Protein Studies
- CRISPR and Genetic Engineering
- Genomics and Chromatin Dynamics
- 3D Printing in Biomedical Research
- RNA modifications and cancer
- Zebrafish Biomedical Research Applications
- Reproductive biology and impacts on aquatic species
- Peptidase Inhibition and Analysis
- Neurogenetic and Muscular Disorders Research
- Tissue Engineering and Regenerative Medicine
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Neuropeptides and Animal Physiology
- Wnt/β-catenin signaling in development and cancer
- Cancer-related molecular mechanisms research
Oregon State University
2013-2024
Tata Institute of Fundamental Research
2012
Howard Hughes Medical Institute
1998-2003
University of California, San Diego
1998-2002
Alleanza Contro il Cancro
2002
Salk Institute for Biological Studies
1999
Scripps Research Institute
1996-1997
Pasteur Hellenic Institute
1991-1995
Max Planck Institute for Biophysical Chemistry
1994-1995
Max Planck Society
1994-1995
Pitx1 is a Bicoid-related homeodomain factor that exhibits preferential expression in the hindlimb, as well developing anterior pituitary gland and first branchial arch. Here, we report gene-deleted mice exhibit striking abnormalities morphogenesis growth of resulting limb structural changes tibia fibula patterning alterations patella proximal tarsus, to more closely resemble corresponding forelimb structures. Deletion locus results decreased distal hindlimb-specific marker, T-box factor,...
CREB binding protein (CBP) functions as an essential coactivator of transcription factors that are inhibited by the adenovirus early gene product E1A. Transcriptional activation signal transducer and activator transcription-1 (STAT1) requires C/H3 domain in CBP, which is primary target E1A inhibition. Here it was found not required for retinoic acid receptor (RAR) function, nor involved Instead, inhibits RAR function preventing assembly CBP-nuclear complexes, revealing differences CBP...
Pax6 , a highly conserved member of the paired homeodomain transcription factor family that plays essential roles in ocular, neural, and pancreatic development effects asymmetric transient dorsal expression during pituitary development, with its extinguished before ventral → appearance specific cell types. Analysis Small eye −/− mouse mutants reveals dorsoventral axis gland becomes ventralized, extension transcriptional determinants types, particularly PFrk . This ventralization is followed...
Pitx2 is a bicoid-related homeodomain factor that required for effective cell type-specific proliferation directly activating specific growth-regulating gene cyclin D2 . Here, we report Pitx2, in response to the Wnt/β-catenin pathway and growth signals, also can regulate c -Myc D1. Investigation of molecular mechanisms Pitx2-dependent proliferation, these cases, further supports nuclear role β-catenin preventing histone deacetylase 1-dependent inhibitory functions several DNA-binding...
Pitx2 expression is observed during all states of the myogenic progression in embryonic muscle anlagen and persists adult muscle. mutant mice form but a few anlagen. Loss or degeneration could generally be attributed to loss attachment site induced by some other aspect phenotype. Muscles derived from first branchial arch were absent, whereas muscles second merely distorted mutants at midgestation. was expressed well before, required for, initiation first, not second, mesoderm. also for...
The search for developmental mechanisms driving vertebrate organogenesis has paved the way toward a deeper understanding of birth defects. During embryogenesis, parts heart and craniofacial muscles arise from pharyngeal mesoderm (PM) progenitors. Here, we reveal hierarchical regulatory network set transcription factors expressed in PM that initiates organogenesis. Genetic perturbation this mice resulted muscle defects, revealing robust cross-regulation between its members. We identified Lhx2...
Xanthohumol (XN), a prenylated flavonoid from hops, improves dysfunctional glucose and lipid metabolism in animal models of metabolic syndrome (MetS). However, its transformation into the estrogenic metabolite, 8-prenylnaringenin (8-PN), poses potential health concern for use humans. To address this concern, we evaluated two hydrogenated derivatives, α,β-dihydro-XN (DXN) tetrahydro-XN (TXN), which showed negligible affinity estrogen receptors α β, cannot be metabolically converted 8-PN. We...
Homeobox genes are regulators of place-dependent morphogenesis and play important roles in controlling the expression patterns cell adhesion molecules (CAMs). To identify proteins that bind to a regulatory element common for two neural CAMs, Ng–CAM L1, we screened mouse cDNA library with concatamer sequence CCATTAGPyGA found new homeobox gene, which have called Barx2 . The homeodomain encoded by is 87% identical Barx1 , both related at Bar locus Drosophila melanogaster also encode an stretch...
Nuclear LIM domains interact with a family of coregulators referred to as Clim/Ldb/Nli. Although one member, Clim-2/Ldb-1/Nli, is highly expressed in epidermal keratinocytes, no nuclear domain factor known be epidermis. Therefore, we used the conserved LIM-interaction Clim screen for factors adult and embryonic mouse skin expression libraries isolated that homologous previously described LIM-only proteins LMO-1, -2, -3. This factor, LMO-4, overlapping manner Clim-2 epidermis several other...
The transcription factor Ctip2/Bcl11b plays essential roles in developmental processes of the immune and central nervous systems skin. Here we show that Ctip2 also a key role tooth development. is highly expressed ectodermal components developing tooth, including inner outer enamel epithelia, stellate reticulum, stratum intermedium, ameloblast cell lineage. In Ctip2(-/-) mice, morphogenesis appeared to proceed normally through cap stage but developed multiple defects at bell stage. Mutant...
Abstract Vitamin E (VitE) deficiency results in embryonic lethality. Knockdown of the gene ttpa encoding for VitE regulatory protein [α-tocopherol transfer (α-TTP)] zebrafish embryos causes death within 24 h post-fertilization (hpf). To test hypothesis that VitE, not just α-TTP, is necessary nervous system development, adult 5D strain zebrafish, fed either sufficient (E+) or deficient (E−) diets, were spawned to obtain E+ and E− embryos, which subjected RNA situ hybridization RT-qPCR. Ttpa...