A5085732836- RNA Research and Splicing
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Neuroblastoma Research and Treatments
- Cancer, Hypoxia, and Metabolism
- Cancer-related molecular mechanisms research
- Cancer Treatment and Pharmacology
- Ion channel regulation and function
- Neuroscience and Neuropharmacology Research
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- Protein Degradation and Inhibitors
- Cancer Genomics and Diagnostics
- Extracellular vesicles in disease
- Cancer, Stress, Anesthesia, and Immune Response
- Ubiquitin and proteasome pathways
- Molecular Biology Techniques and Applications
- Pharmacological Receptor Mechanisms and Effects
- DNA and Nucleic Acid Chemistry
- Neurogenetic and Muscular Disorders Research
- Neuropeptides and Animal Physiology
- Cell death mechanisms and regulation
- Amyotrophic Lateral Sclerosis Research
- Mitochondrial Function and Pathology
- Advanced biosensing and bioanalysis techniques
University of Trento
2015-2024
University of Lausanne
2021
University of Padua
2015
University of Florence
1999-2014
Interuniversity Consortium for Magnetic Resonance
2006-2014
Genomics (United Kingdom)
2012-2014
University of Milan
1997-2014
Hospital of Prato
2007
Casa Sollievo della Sofferenza
2003-2006
Florence (Netherlands)
2006
The BioMart Community Portal (www.biomart.org) is a community-driven effort to provide unified interface biomedical databases that are distributed worldwide. portal provides access numerous database projects supported by 30 scientific organizations. It includes over 800 different biological datasets spanning genomics, proteomics, model organisms, cancer data, ontology information and more. All resources available through the independently administered funded their host data federation...
Spices and herbs often contain active phenolic substances endowed with potent antioxidative properties. We had previously shown that curcumin, the yellow pigment in curry, strongly induced HO-1 expression activity rat astrocytes. In CNS, has been reported to operate as a fundamental defensive mechanism for neurons exposed an oxidant challenge. Treatment of astrocytes curcumin upregulated protein at both cytoplasmic nuclear levels, by immunofluorescence analysis under laser-scanning confocal...
The CDKN1B gene encodes the cyclin-dependent kinase inhibitor p27KIP1, an atypical tumor suppressor playing a key role in cell cycle regulation, proliferation, and differentiation. Impaired p27KIP1 expression and/or localization are often observed cells, further confirming its central regulating cycle. Recently, germline mutations have been associated with inherited multiple endocrine neoplasia syndrome type 4, autosomal dominant characterized by varying combinations of tumors affecting at...
Abstract Background The classical view on eukaryotic gene expression proposes the scheme of a forward flow for which fluctuations in mRNA levels upon stimulus contribute to determine variations availability translation. Here we address this issue by simultaneously profiling with microarrays total mRNAs (the transcriptome) and polysome-associated translatome) after EGF treatment human cells, extending analysis other 19 different transcriptome/translatome comparisons mammalian cells following...
Three-dimensional (3D) porous scaffolds combined with therapeutic stem cells play vital roles in tissue engineering. The adult brain has very limited regeneration ability after injuries such as trauma and stroke. In this study, injectable 3D silk fibroin-based hydrogel encapsulated neural were developed, aiming at supporting regeneration. To improve the function of towards cells, fibroin was modified by an IKVAV peptide through covalent binding. Both unmodified hydrogels obtained,...
Genetic alterations impacting ubiquitously expressed proteins involved in RNA metabolism often result neurodegenerative conditions, with increasing evidence suggesting that translation defects can contribute to disease. Spinal muscular atrophy (SMA) is a neuromuscular disease caused by low levels of SMN protein, whose role pathogenesis remains unclear. Here, we identified vivo and vitro are cell autonomous dependent. By determining parallel the transcriptome translatome SMA mice, observed...
Abstract N6‐methyladenosine (m 6 A) regulates a variety of physiological processes through modulation RNA metabolism. This modification is particularly enriched in the nervous system several species, and its dysregulation has been associated with neurodevelopmental defects neural dysfunctions. In Drosophila , loss m A alters fly behavior, albeit underlying molecular mechanism role during development have remained elusive. Here we find that impairment pathway leads to axonal overgrowth...
Glioblastoma stem cells (GSCs) resist current glioblastoma (GBM) therapies. GSCs rely highly on oxidative phosphorylation (OXPHOS), whose function requires mitochondrial translation. Here we explore the therapeutic potential of targeting translation and report results high-content screening with putative blockers ribosomes. We identify bacterial antibiotic quinupristin/dalfopristin (Q/D) as an effective suppressor GSC growth. Q/D also decreases clonogenicity in vitro, consequently...
YTHDF proteins bind the N6-methyladenosine (m6A)-modified mRNAs, influencing their processing, stability, and translation. Therefore, members of this protein family play crucial roles in gene regulation several physiological pathophysiological conditions. contain a hydrophobic pocket that accommodates m6A embedded RRACH consensus sequence on mRNAs. We exploited presence cage to set up an m6A-competitive assay performed high-throughput screen aimed at identifying ligands binding pocket....
Neuronal ELAV-like proteins (HuB, HuC, and HuD) are highly conserved RNA-binding able to selectively associate with the 3' UTR of a subset target mRNAs increase their cytoplasmic stability rate translation. We previously demonstrated involvement these in learning, reporting that they undergo sustained up-regulation hippocampus mice trained spatial discrimination task. Here, we extend this finding, showing similar occurs rats another learning paradigm, Morris water maze. HuD, strictly...
The view that memory is encoded by variations in the strength of synapses implies long-term biochemical changes take place within subcellular microdomains neurons. These are thought ultimately to be an effect transcriptional regulation specific genes. Localized changes, however, cannot fully explained a purely control gene expression. neuron-specific ELAV-like HuB, HuC, and HuD RNA-binding proteins act posttranscriptionally binding adenine- uridine-rich elements (AREs) 3' untranslated region...
Tumour onset and progression are due to the accumulation of genomic lesions, which alter gene expression ultimately proteome activities. These lesions thought affect primarily transcriptional control expression. In present study, we aimed at evaluating genome-wide occurrence alterations in translational exploiting an isogenic, phenotypically validated cellular model colorectal cancer (CRC) transition from invasive carcinoma metastasis. this model, microarray profiling shows that changes...
More than 1 in 20 human genes bear the mRNA 3′ UTR a specific motif called adenine- and uridine-rich element (ARE), which posttranscriptionally determines its expression response to cell environmental signals. ELAV (embryonic lethal abnormal vision) proteins are only known ARE-binding factors that able stabilize bound mRNAs, thereby positively controlling gene expression. Here, we show neuroblastoma SH-SY5Y cells, neuron-specific (nELAV) (HuB, HuC, HuD) up-regulated redistributed by 15 min...
CDK5R1 encodes p35, a specific activator of the serine/threonine kinase CDK5, which plays crucial roles in CNS development and maintenance. CDK5 activity strongly depends on p35 levels p35/CDK5 misregulation is deleterious for correct function, suggesting that tightly controlled regulation expression needed proper activity. Accordingly, was demonstrated to be at both transcriptional post-transcriptional levels, but possible through microRNAs (miRNAs) has never been investigated. We...
Abstract Insulin regulates glycaemia, lipogenesis and increases mRNA translation. Cells with reduced eukaryotic initiation factor 6 (eIF6) do not increase translation in response to insulin. The role of insulin-regulated is unknown. Here we show that reduction mice heterozygous for eIF6 results normal but less blood cholesterol triglycerides. controls fatty acid synthesis glycolysis a cell autonomous fashion. acts by exerting translational control adipogenic transcription factors like...
Abstract Background HuR, an RNA binding protein involved in the post-transcriptional regulation of a wide spectrum mRNAs, has been demonstrated to be determinant carcinogenesis and tumor aggressiveness several cancer types. In this study, we investigated role HuR apoptosis chemoresistance induced by widely used anticancer drug doxorubicin human breast cells (MCF-7). Results We showed that acts early phase cell response doxorubicin, being translocate into cytoplasm upon phosphorylation....
The Human antigen R protein (HuR) is an RNA-binding that recognizes U/AU-rich elements in diverse RNAs through two RNA-recognition motifs, RRM1 and RRM2, post-transcriptionally regulates the fate of target RNAs. natural product dihydrotanshinone-I (DHTS) prevents association HuR vitro cultured cells by interfering with binding to RNA. Here, we report structural determinants interaction between DHTS impact on mRNAs transcriptome-wide. NMR titration Molecular Dynamics simulation identified...
Post-transcriptional regulation (PTR) of gene expression is now recognized as a major determinant cell phenotypes. The recent availability methods to map protein-RNA interactions in entire transcriptomes such RIP, CLIP and their variants, together with global polysomal ribosome profiling techniques, are driving the exponential accumulation vast amounts data on mRNA contacts cells, corresponding predictions PTR events. However, this exceptional quantity information cannot be exploited at its...
Highlights•RiboLace isolates ribosomes in active translation by antibody-free and tag-free pull-down•RiboLace works reliably with low amounts of input material vitro vivo•RiboLace provides positional data nucleotide resolution•RiboLace estimates levels predicts protein accuracySummaryRibosome profiling, or Ribo-seq, is based on large-scale sequencing RNA fragments protected from nuclease digestion ribosomes. Thanks to its unique ability provide information about flowing along transcripts,...