A5077568042- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Alzheimer's disease research and treatments
- Diabetes and associated disorders
- Ocular Oncology and Treatments
- Immunodeficiency and Autoimmune Disorders
- Monoclonal and Polyclonal Antibodies Research
- Dementia and Cognitive Impairment Research
- Advanced Glycation End Products research
- Cancer Cells and Metastasis
- Hemoglobinopathies and Related Disorders
- Pancreatic function and diabetes
- Celiac Disease Research and Management
- Ubiquitin and proteasome pathways
- Poxvirus research and outbreaks
- RNA and protein synthesis mechanisms
- Corneal Surgery and Treatments
- Italian Social Issues and Migration
- Retinal Development and Disorders
- Down syndrome and intellectual disability research
- Nuclear Receptors and Signaling
- Erythrocyte Function and Pathophysiology
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Curcumin's Biomedical Applications
Ospedale Policlinico San Martino
1986-2018
Alleanza Contro il Cancro
1991-2015
National Cancer Research Institute
2008-2013
Istituti di Ricovero e Cura a Carattere Scientifico
2013
Griffith University
2002
Vita-Salute San Raffaele University
2002
University of Genoa
1996-2000
Case Western Reserve University
1998
Center for Human Genetics
1998
Martin University
1996
T lymphocytes are defective in cystine uptake and thus require exogenous thiols for activation function. Here we show that monocyte-derived human dendritic cells (DCs) release cysteine the extracellular space. Cysteine generation is increased by lipopolysaccharide tumor necrosis factor α, contact with specifically recognizing soluble or alloantigens. These stimuli also induce thioredoxin (TRX) accumulation DCs. However, only antigen-specific triggers TRX secretion antigen-presenting cells....
Abstract Allelic sequence variation of the HLA DP-alpha and DP-beta genes has been analyzed in a panel 34 DP-typed cell lines. The polymorphic second exon these was specifically amplified vitro by polymerase chain reaction method, using thermostable DNA Thermus, aquaticus. analysis M13 clones containing sequences revealed total 14 allelic variants. In general, specific were associated with each defined DPw1-w6 types, beta allele subtypes for DPw2 DPw4 specificities. An additional six alleles...
Emerging evidence suggests that metformin, a widely used anti-diabetic drug, may be useful in the prevention and treatment of different cancers. In present study, we demonstrate metformin directly inhibits enzymatic function hexokinase (HK) I II cell line triple-negative breast cancer (MDA-MB-231). The inhibition is selective for these isoforms, as documented by experiments with purified HK well lysates. Measurements (18)F-fluoro-deoxyglycose uptake document it dose- time-dependent powerful...
Uveal melanoma is the most frequent primary tumour of eye. It molecularly clearly distinct from cutaneous and shows a different pattern driver mutations. The influence sunlight ultraviolet (UV) exposure on aetiology uveal matter debate. recent identification mutations in promoter telomerase reverse transcriptase (TERT) gene with UV-induced cytidine-to-thymidine transitions prompted us to investigate whether these also occur melanoma. We analysed 50 cases obtained enucleation surgery for...
The human major histocompatibility complex class II antigens of the HLA-D are highly polymorphic, surface proteins essential in cellular interactions necessary for an immune response. analysis this polymorphism is crucial (i) matching transplantation and (ii) understanding association between certain important diseases. haplotypes may escape detection by current methodologies. Analysis at genomic level one subregions HLA-DR, using oligonucleotide probes specific polymorphic regions, capable...
Uveal melanoma is an aggressive cancer that metastasizes to the liver in about half of patients, with a high lethality rate. Identification patients at risk metastases may provide indication for frequent follow-up early detection and treatment. The analysis gene expression profiles primary human uveal melanomas showed SDCBP (encoding syndecan-binding protein-1 or mda-9/syntenin), which appeared higher recurrence, whereas syndecans was lower unrelated progression. Moreover, we found related...
Progressive supranuclear palsy (PSP) is a neurodegenerative disorder characterized by extensive neurofibrillary tangle (NFT) formation and neuronal loss in selective populations. Currently, no clues to the biological events underlying pathological process have emerged. In Alzheimer disease (AD), which shares with PSP occurrence of NFTs, advanced glycation end products (AGEs) as well oxidation adducts been found be increased association pathology. The presence amount lipid protein markers,...
We have characterized the interaction between apolipoprotein E (apoE) and amyloid β peptide (Aβ) in soluble fraction of cerebral cortex Alzheimer’s disease (AD) control subjects. Western blot analysis with specific antibodies identified both groups a complex composed full-length apoE Aβ peptides ending at residues 40 42. The apoE–Aβ aggregate is less stable AD brains than controls, when treated anionic detergent SDS. present significantly higher quantity brains, whereas insoluble an inverse...
Abstract Resting T lymphocytes can internalize reduced cysteine (Cys) but not cystine, the oxidized form of amino acid that predominates extracellularly. In vitro studies have shown DC provide Cys to cells during antigen presentation, allowing their activation. Here, we show increased thiol production is a hallmark immune response in vivo . Indeed, content LN increases dramatically after injection. Non‐protein thiols co‐distribute with and are highly abundant germinal centers. agreement,...
Curcumin induces apoptosis in many cancer cells and it reduces xenograft growth the formation of lung metastases nude mice. Moreover, plant derived polyphenol has been reported to be able overcome drug resistance classical chemotherapy. These features render a promising candidate for tumor therapy especially cancers known their high rates concerning like melanoma. We show here that melanoma cell line M14 is resistant induced apoptosis, which correlates with absence any effect on NFκB...
Observations on the role of ovarian hormones in breast cancer growth, as well interest contraception, stimulated research into biology estrogens. The identification classical receptors ERα and ERβ transmembrane receptor GPER resolution structure ligand bound to its established principal molecular mechanisms estrogen action. presence estrogen-like compounds many plants used traditional medicine or ingested food ingredients, phytoestrogens, estrogenic activities industrial pollutants...
Polymorphism is a hallmark of the molecules encoded within major histocompatibility complex (MHC) humans and other mammals. The exact role this polymorphism still unknown, but it may be to enhance capacity immune system respond wide variety antigens. For number years, our laboratory has been interested in defining structural basis serologic specificities that map HLA-D region human complex. We demonstrated previously HLA-DQ subregion contained genes encoding α- β-chains molecule (Giles et...
// Adriana Amaro 1, * , Giovanna Angelini Valentina Mirisola 1 Alessia Isabella Esposito Daniele Reverberi Serena Matis Massimo Maffei Walter Giaretti Maurizio Viale 2 Rosaria Gangemi Laura Emionite 3 Simonetta Astigiano 4 Michele Cilli Beatrice E. Bachmeier 5 Peter H. Killian Albini 6 Ulrich Pfeffer Molecular Pathology, IRCCS AOU San Martino – IST Istituto Nazionale per la Ricerca sul Cancro, Genova, Italy Biotherapy, Animal Facility, Immunology, Institute of Laboratory Medicine,...
Abstract Interaction of NK cells with autologous immature dendritic (iDCs) results in reciprocal activation. We have previously reported that trigger iDC to polarize and secrete IL-18; turn, DC-activated the nuclear protein/proinflammatory cytokine high mobility group box protein 1 (HMGB1), which induces DC maturation prevents from lysis. However, activated can also kill iDC. To investigate whether effector maturative properties may coexist or segregate different subsets, human cell clones...
Several substances widely dispersed in the environment including hormones, industrial by-products and pollutants exert hormone like activity affecting steroid-responsive physiological systems. These compounds, named endocrine disruptors, are suspected to affect mammalian reproductive system. However it is still unclear whether these able elicit estrogen at low concentrations encountered environment. Here we compare effects of disruptor nonylphenol with elicited by 17-β-estradiol on gene...