A5103145736- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Influenza Virus Research Studies
- Immunodeficiency and Autoimmune Disorders
- Diabetes and associated disorders
- Reproductive System and Pregnancy
- Estrogen and related hormone effects
- Hematopoietic Stem Cell Transplantation
- Cell Adhesion Molecules Research
- CAR-T cell therapy research
- Platelet Disorders and Treatments
- COVID-19 epidemiological studies
- Blood groups and transfusion
- vaccines and immunoinformatics approaches
- Respiratory viral infections research
- Growth Hormone and Insulin-like Growth Factors
- Immune Response and Inflammation
- RNA Research and Splicing
- Cytomegalovirus and herpesvirus research
- Virology and Viral Diseases
- Virus-based gene therapy research
- Single-cell and spatial transcriptomics
- Receptor Mechanisms and Signaling
Versiti Blood Center of Wisconsin
2011-2024
Medical College of Wisconsin
1998-2022
Laboratory of Molecular Genetics
2012
University of Massachusetts Chan Medical School
2007
The San Raffaele Telethon Institute for Gene Therapy
2002
Fondazione Salvatore Maugeri
1999
University of Wisconsin–Madison
1984-1999
Ospedale Policlinico San Martino
1999
University of Naples Federico II
1999
University of Missouri
1999
An antibody to a platelet integral membrane glycoprotein was found cross-react with the previously identified CD31 myelomonocytic differentiation antigen and hec7, an endothelial cell protein that is enriched at intercellular junctions. This complementary DNA clone from library. The 130-kilodalton translated sequence contained six extracellular immunoglobulin (Ig)-like domains most similar adhesion molecule (CAM) subgroup of Ig superfamily. only known member CAM family on platelets. Its...
The analysis of the T cell repertoires involved in local or systemic immune responses is beginning to play an important role many clinical situations. These include autoimmunity, response viral bacterial superantigens, alloimmunity including allograft rejection, and tumor immunity. Here we analyze circulating by determining TCR beta-chain gene complexity using a modification V beta family-specific PCR. This approach, called CDR3 size spectratyping, uses heterogeneity as further source...
Human platelets are derived from megakaryocytes as anucleate cells, and thus contain only vestigial amounts of RNA capable being transcribed into protein. This has greatly hampered efforts to study directly platelet-specific gene products their associated polymorphisms. In this report, we describe direct amplification, using the polymerase chain reaction, platelet-derived mRNA in sufficient permit detailed analysis, such restriction mapping nucleotide sequencing. The ability generate large...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTEquilibrium binding of estradiol by uterine cell suspensions and whole uteri in vitroDavid Williams Jack GorskiCite this: Biochemistry 1974, 13, 27, 5537–5542Publication Date (Print):December 1, 1974Publication History Published online1 May 2002Published inissue 1 December 1974https://pubs.acs.org/doi/10.1021/bi00724a013https://doi.org/10.1021/bi00724a013research-articleACS PublicationsRequest reuse permissionsArticle...
The predominant class II, or Ia, antigen of the human major histocompatibility complex is HLA-DR. It consists an alpha and a beta chain, latter being responsible for remarkable polymorphism these Ia antigens. Studies with cloned genes had shown existence more than one DR beta-chain locus. We have isolated about 100 kilobases HLA-DR gene region from cosmid library generated consanguineous homozygous B-cell line DR3 haplotype. Three been characterized. They are arranged in head-to-tail...
The human major histocompatibility complex class II antigens of the HLA-D are highly polymorphic, surface proteins essential in cellular interactions necessary for an immune response. analysis this polymorphism is crucial (i) matching transplantation and (ii) understanding association between certain important diseases. haplotypes may escape detection by current methodologies. Analysis at genomic level one subregions HLA-DR, using oligonucleotide probes specific polymorphic regions, capable...
Phospholipase Cγ1 (PLCγ1) is an important signaling effector of T cell receptor (TCR). To investigate the role PLCγ1 in biology, we generated and examined mice with cell–specific deletion PLCγ1. We demonstrate that deficiency affects positive negative selection, significantly reduces single-positive thymocytes peripheral cells, impairs TCR-induced proliferation cytokine production, activation ERK, JNK, AP-1, NFAT, NF-κB. Importantly, development function FoxP3+ regulatory causing...
The class II products of the major histocompatibility complex, also called Ia antigens, are composed two polypeptide chains, alpha and beta both encoded within complex. In man, antigens can be divided into three biochemically distinct groups HLA-DR, HLA-DC, HLA-SB. Our isolation cDNA clones for polymorphic chain HLA-DR HLA-DC has allowed us to study organization genes. Here we identify HLA-SB beta-chain gene in recombinant from a cosmid library generated consanguineous homozygous B-cell...
Recent progress in the molecular biology of human major histocompatibility complex class II genes (HLA-DP, -DQ, -DR) have shown that genetic complexity and allelic polymorphism are greater than expected. In case HLA-DR, three DR beta-chain loci been identified linked, two which (DR beta I III, now assigned names HLA-DRIB HLA-DR3B) functional. We HLA micropolymorphism detected at DNA sequence level can easily be analyzed by hybridization with allele-specific oligonucleotides (HLA...
In this study, we analyze the recall response to influenza A matrix peptide M1(58-66) restricted by HLA-A2 in one individual and find a strict CDR3 selection as well high degree of polyclonality. The TCR beta-chain repertoire memory T cells specific for Ag system has been shown previously be constrained use BV17 family I/sRS(A)/S amino acid motif region. Our sequence analysis from CTL line showed highly polyclonal, 95 distinct sequences (clonotypes) were identified expressing motif....
Abstract The nature of CD8+ T cell memory is still incompletely understood. We have previously reported that the response to an HLA-A2-restricted influenza-derived peptide results in a complex repertoire. In this study we extend analysis and describe repertoire with more rigor. one individual defined 141 distinct clonotypes on basis unique DNA sequence third complementarity-determining region TCR β-chain. frequency distribution not what expected normal but characterized by large...
Abstract Interferon-γ has been shown to be important for the resolution of inflammation associated with CNS autoimmunity. Because one roles γδ T cells is regulation inflammation, we asked whether were able regulate using autoimmune disease mouse model experimental encephalomyelitis (EAE). We show that presence was needed promote production IFN-γ by both CD4 and CD8 in before onset EAE. This independent ability produce IFN-γ, specific CNS, as no alterations detectable cell-deficient mice...