A5025457197- Retinoids in leukemia and cellular processes
- Cancer, Lipids, and Metabolism
- Angiogenesis and VEGF in Cancer
- Immune Cell Function and Interaction
- Carbohydrate Chemistry and Synthesis
- Inflammatory mediators and NSAID effects
- Natural product bioactivities and synthesis
- Monoclonal and Polyclonal Antibodies Research
- Neonatal Respiratory Health Research
- Cancer, Hypoxia, and Metabolism
- Genomics, phytochemicals, and oxidative stress
- Antioxidant Activity and Oxidative Stress
- Immunotherapy and Immune Responses
- PI3K/AKT/mTOR signaling in cancer
- Estrogen and related hormone effects
- Ocular Oncology and Treatments
- Cancer, Stress, Anesthesia, and Immune Response
- Endoplasmic Reticulum Stress and Disease
- Radiopharmaceutical Chemistry and Applications
- Neurogenetic and Muscular Disorders Research
- Renal function and acid-base balance
- Retinal Diseases and Treatments
- Colorectal Cancer Treatments and Studies
- Prostate Cancer Treatment and Research
- HER2/EGFR in Cancer Research
Ospedale Policlinico San Martino
2013-2023
Istituto Giannina Gaslini
2022
Molecular Oncology (United States)
2007-2021
Alleanza Contro il Cancro
1994-2020
Istituti di Ricovero e Cura a Carattere Scientifico
2012-2014
Agence Nationale de la Recherche
2014
MultiMedica
2008-2011
University of Milan
2011
University of Insubria
2006-2011
National Cancer Research Institute
2002-2008
Abstract Background CTLA-4 (Cytotoxic T lymphocyte antigen-4) is traditionally known as a negative regulator of cell activation. The blocking using human monoclonal antibodies, such Ipilimumab, currently used to relieve CTLA-4-mediated inhibition anti-tumor immune response in metastatic melanoma. Herein, we have analyzed expression and Ipilimumab reactivity on melanoma lines tumor tissues from cutaneous patients. Then, investigated whether can trigger innate immunity terms antibody dependent...
Here we report that human nonsmall cell lung carcinomas overexpress macrophage migration inhibitory factor (MIF) and thioredoxin (Trx), 2 oxidoreductases with cytokine function, contain more abundant nonprotein thiols (glutathione cysteine) than nonneoplastic tissues. Cell clones derived from the same carcinoma lines but expressing different levels of Trx and/or MIF displayed growth rates in vitro vivo correlating not MIF. Interestingly, generate extracellularly reduced thiols, amounts...
Despite recent advances in understanding the biological basis of prostate cancer, management disease, especially phase resistant to androgen ablation, remains a significant challenge. The long latency and high incidence carcinogenesis provides opportunity intervene with chemoprevention prevent or eradicate malignancies. In this study, we have used human hormone-resistant cancer cells, DU145 PC3, as an vitro model assess efficacy xanthohumol (XN) against cell growth, motility invasion. We...
Abstract The oncogenic Bcr-Abl tyrosine kinase activates various signaling pathways including phosphoinositide 3-kinase/Akt and nuclear factor-κB that mediate proliferation, transformation, apoptosis resistance in Bcr-Abl(+) myeloid leukemia cells. hop flavonoid xanthohumol inhibits tumor growth by targeting the Akt angiogenesis. Here, we show has vitro activity against cells clinical samples retained its cytotoxicity when imatinib mesylate–resistant K562 were examined. Xanthohumol...
Amino-bis-phosphonates (N-BPs) such as zoledronate (Zol) have been used in anticancer clinical trials due to their ability upregulate pyrophosphate accumulation promoting antitumor Vγ9Vδ2 T cells. The butyrophilin 3A (BTN3A, CD277) family, mainly the BTN3A1 isoform, has emerged an important structure contributing cells stimulation. It demonstrated that B30.2 domain of can bind phosphoantigens (PAg) and drive activation through conformational changes extracellular domains. Moreover, binding...
Hodgkin lymphoma (HL) resistant to conventional therapies is increasing, making of interest the search for new schemes treatment. Members "A Disintegrin And Metalloproteases" (ADAMs) family, mainly ADAM10 or ADAM17, have been proposed as therapeutic targets in solid tumors and some ADAMs inhibitors shown exert antitumor effects. We previously described an overexpression HL, together with increased release NKG2D ligands (NKG2D-L) reduced activation effector T lymphocytes anti-lymphoma...
Retinol binding protein (RBP), the retinol-specific carrier, circulates in blood as a 1:1 complex with homotetrameric transthyretin (TTR). Both RBP and TTR are synthesized secreted by hepatocyte. In this work we have demonstrated, using HepG2 cells model system, that association between two proteins occurs inside cell before secretion. The intracellular was detected only when metabolically labeled were lysed under mild detergent conditions (1.5% octylglucoside), followed immunoprecipitation...
Abstract Background Prostate cancer shows an extremely slow progression, appearing in its metastatic, hormone refractory phenotype mostly elderly men. The chemopreventive targeting of this tumor could accordingly delay malignancy over life expectancy. retinoid N -(4 hydroxyphenyl)retinamide (4HPR) has already been shown to restrain prostate growth vitro and vivo, though mechanisms action are only partially explained. Results We found that 4HPR impairs DU145 PC3 cells migration invasion by...
Shedding of ADAM10 substrates, like TNFα, MICA or CD30, is reported to affect both anti-tumor immune response and antibody-drug-conjugate (ADC)-based immunotherapy. Soluble forms these molecules can be carried spread in the microenvironment by exosomes released tumor cells. We new inhibitors able prevent shedding Hodgkin lymphoma (HL), leading recognition HL cells cytotoxic lymphocytes. In this paper, we show that mature bioactive form exosome-like vesicles (ExoV) lymph node mesenchymal...
Background Antibody-drug conjugates (ADC) are essential therapeutic options to treat solid and hematological cancers. The anti-epidermal growth factor-receptor (EGFR) antibody cetuximab (Cet) is used for the therapy of colorectal carcinoma (CRC). Anti-CRC Vδ2 cytolytic T lymphocytes can be elicited by priming tumor cells with aminobisphosphonate zoledronic acid (ZA) consequent presentation isopentenyl pyrophosphates through butyrophilin (BTN) family members such as BTN3A1 BTN2A1. A major...
The p38 inhibitor SB202190 is a necessary component of the medium used for normal colorectal mucosa cultures. Sato et al. suggested that primary activity may be EGFR signaling stabilization, causing an increased phosphorylation Erk1-2 sustaining organoid proliferation. However, growth some cancer (CRC)-derived cultures inhibited by this molecule via unknown mechanism. We biochemically investigated on collection 25 human CRC organoids, evaluating EGFR, Akt and activation using Western blot....
Abstract The induction of programmed cell death in premalignant or malignant cancer cells by chemopreventive agents could be a valuable tool to control prostate initiation and progression. In this work, we present evidence that the C-28 methyl ester synthetic oleanane triterpenoid 2-cyano-3,12-dioxooleana-1,9(11)-dien-28-oic acid (CDDO-Me) induces androgen-responsive unresponsive human lines at nanomolar low micromolar concentrations. CDDO-Me induced caspase-3, caspase-8, caspase-9...
Autoimmune lymphoproliferative syndrome (ALPS) is an inherited disorder of lymphocyte homeostasis classically due to mutation FAS, FASL, and CASP10 genes (ALPS-FAS/CASP10). Despite recent progress, about one-third ALPS patients does not carry classical mutations still remains gene orphan (ALPS-U, undetermined genetic defects). The aims the present study were compare clinical immunological features ALPS-FAS/CASP10 versus those ALPS-U affected subjects deepen characteristics this latter group....
Following damage to skeletal muscle, satellite cells become activated, migrate towards the injured area, proliferate, and fuse with each other form myotubes which finally mature into myofibers. We tested a new approach muscle regeneration by incorporating myoblasts, or without exogenous growth factors bFGF HGF, three-dimensional gels of reconstituted basement membrane (matrigel). In vitro, HGF induced C2C12 myoblast proliferation migration were synergistic when used together. vivo, primary...