A5068799456- Chronic Lymphocytic Leukemia Research
- Lung Cancer Treatments and Mutations
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Cancer Genomics and Diagnostics
- Radiomics and Machine Learning in Medical Imaging
- Monoclonal and Polyclonal Antibodies Research
- Genetic factors in colorectal cancer
- Immunodeficiency and Autoimmune Disorders
- Colorectal Cancer Treatments and Studies
- Lymphoma Diagnosis and Treatment
- Thyroid Cancer Diagnosis and Treatment
- RNA modifications and cancer
- MicroRNA in disease regulation
- Cancer-related Molecular Pathways
- Colorectal and Anal Carcinomas
- Glioma Diagnosis and Treatment
- Calcium signaling and nucleotide metabolism
- Immune Response and Inflammation
- Lung Cancer Research Studies
- Extracellular vesicles in disease
- Medical Imaging Techniques and Applications
- Retinal Development and Disorders
- Multiple Sclerosis Research Studies
Ospedale Policlinico San Martino
2013-2024
CTO Andrea Alesini
2008
Alleanza Contro il Cancro
1992-2007
Azienda Ospedaliera Sant'Andrea
2006-2007
Center for Cancer Research
2004-2005
University of Ferrara
2004-2005
Sidney Kimmel Cancer Center
2004
North Shore University Hospital
1994-2004
New York University
2004
Thomas Jefferson University
2004
Chronic lymphocytic leukemia (CLL) is the most common human and characterized by predominantly nondividing malignant B cells overexpressing antiapoptotic cell lymphoma 2 (Bcl2) protein. miR-15a miR-16-1 are deleted or down-regulated in majority of CLLs. Here, we demonstrate that expression inversely correlated to Bcl2 CLL both microRNAs negatively regulate at a posttranscriptional level. BCL2 repression these induces apoptopsis leukemic line model. Therefore, miR-15 miR-16 natural antisense...
Little is known about the expression levels or function of micro-RNAs (miRNAs) in normal and neoplastic cells, although it becoming clear that miRNAs play important roles regulation gene during development [Ambros, V. (2003) Cell 113, 673-676; McManus, M. T. Semin. Cancer Biol. 13, 253-258]. We now report genomewide profiling human B cell chronic lymphocytic leukemia (CLL) by using a microarray containing hundreds precursor mature miRNA oligonucleotide probes. This approach allowed us to...
MicroRNAs (miRNAs) are a class of small noncoding RNA genes recently found to be abnormally expressed in several types cancer. Here, we describe developed methodology for miRNA gene expression profiling based on the development microchip containing oligonucleotides corresponding 245 miRNAs from human and mouse genomes. We used these microarrays obtain highly reproducible results that revealed tissue-specific signatures, data were confirmed by assessment Northern blots, real-time RT-PCR,...
Abstract The accumulation of B lymphocyte clones in the cerebrospinal fluid (CSF) patients with multiple sclerosis (MS) and other neurological disorders was investigated using PCR technologies. Oligoclonal cell accumulations were detected 10 MS patients, but only 3 disorders. Analyses Ig V(D)J sequences on CSF from disclosed that VH3 VH4 genes extensively mutated compared germline sequences. Moreover, a substantial proportion molecular analyzed shared same third CDR H chain variable region...
Studies of B cell antigen receptors (BCRs) expressed by leukemic lymphocytes from patients with chronic lymphocytic leukemia (B-CLL) suggest that some level BCR structural restriction become transformed. While analyzing rearranged VHDJH and VLJL genes 25 non–IgM-producing B-CLL cases, we found five IgG+ cases display strikingly similar BCRs (use the same H- L-chain V gene segments unique, shared heavy chain third complementarity-determining region [HCDR3] light [LCDR3] motifs). These...
Uveal melanoma is the most frequent primary tumour of eye. It molecularly clearly distinct from cutaneous and shows a different pattern driver mutations. The influence sunlight ultraviolet (UV) exposure on aetiology uveal matter debate. recent identification mutations in promoter telomerase reverse transcriptase (TERT) gene with UV-induced cytidine-to-thymidine transitions prompted us to investigate whether these also occur melanoma. We analysed 50 cases obtained enucleation surgery for...
Radiomics is defined as the use of automated or semi-automated post-processing and analysis multiple features derived from imaging exams. Extracted might generate models able to predict molecular profile solid tumors. The aim this study was develop a predictive algorithm define mutational status EGFR in treatment-naïve patients with advanced non-small cell lung cancer (NSCLC). CT scans 109 NSCLC (21 EGFR-mutant 88 EGFR-wild type) underwent radiomics machine learning model recognize EGFR-WT...
Abstract The present study demonstrates that an agonistic anti‐CD38 monoclonal antibody (mAb) (IB4) is capable of preventing apoptosis human tonsillar germinal center (GC) B cells as measured by either morphological methods on Giemsa‐stained cytospin preparations or flow cytometry propidium iodidestained cells. Two other mAb (Leu‐17 and OKT10) consistently failed to prevent in the same cells, even when tested over a wide range concentrations. Furthermore, exposure GC IB4 up‐regulates bcl‐2...
Chronic lymphocytic leukemia (CLL) is characterized by the clonal expansion of CD5-expressing B lymphocytes. Most studies have found that these leukemic CD5+ cells, like their normal counterparts, use immunoglobulin (Ig) variable (V) region genes exhibit minimal, if any, somatic diversity. These and other observations suggested cells may be incapable generating Ig V gene diversity, therefore not able to develop higher affinity binding sites could selected antigen. However, most CLL focused...
The management of non-small cell lung cancer (NSCLC) has become increasingly complex due to the evolution personalized medicine approaches. Such approaches are characterized by necessity adequate tumor samples; hence, improved biopsy techniques needed. Transbronchial cryobiopsy is a novel endoscopic procedure designed collect peripheral pulmonary tissue, and it currently employed in interstitial diseases. use this technique oncology might result mediastinum staging molecular...
Abstract The VH4 genes expressed by both resting and in vivo-activated subepithelial (SE) B cells from human tonsils were studied. Resting SE subdivided according to the presence (IgDlow) or absence (IgM-only) of surface IgD. CD27 was abundant on activated low IgM-only cells. IgDlow could be into CD27low CD27high cell fractions. displayed with a substantial number mutations (13/29 molecular clones mutated), whereas 25/26 unmutated. Moreover, mutated detected mainly within fraction Several...
note that Fig. 1C incorrectly shows the direct correlation between Bc12 levels and of miR-15a miR-16-1 instead indirect correlation, as presented in article.
Abstract Background Meningiomas are mainly benign brain tumors, although about 20% of histologically cases clinically aggressive and recur after resection. We hypothesize that meningioma invasiveness recurrence may be related to the presence cancer stem cells their high responsiveness CXCL12-CXCR4/CXCR7 chemokine axis. The aim this study was isolate from human samples, characterize them for biological features malignant behavior, identify role CXCR4/CXCR7 in these processes. Methods...
This study describes the purification of a subset tonsillar B cells which share phenotypic, morphologic and cytochemical features with subepithelial (SE) cells. These cells, represented 5-10% total were found in Percoll gradient fraction highest density, together resting follicular mantle (FM) The latter however, expressed surface CD5 could be removed by an immune rosetting procedure. remaining small CD5- had phenotype (IgM+, IgD+, CD23-, CD38+/-, CD10-, CD44+) that was different from FM...
ZAP-70 is a protein tyrosine kinase initially found in T and NK cells. Recently, this important signaling element was detected leukemic B cells from subgroup of patients with cell chronic lymphocytic leukemia (B-CLL). In study, normal human tonsils, but not peripheral blood. The cDNA sequence the same as that Germinal center plasma had substantial proportion ZAP-70+ cells, while memory follicular mantle which contain low numbers activated expressed relatively little ZAP-70. A fraction IgD+,...
Mismatch repair (MMR) testing on all new cases of colorectal cancer (CRC) has customarily been preferably performed surgical specimens, as more tissue is available; however, clinical trials for the use immune checkpoint inhibitors in neoadjuvant setting require MMR biopsy samples. This study aims at identifying advantages, disadvantages and any potential pitfalls evaluation how to cope with them. The prospective-retrospective, recruiting 141 biopsies (86 proficient (p)MMR 55 deficient...
The Lynch syndrome (LS) screening algorithm requires BRAF testing as a fundamental step to distinguish sporadic from LS-associated colorectal carcinomas (CRC). by immunohistochemistry (IHC) has shown variable results in the literature. Our aim was analyse concordance between
Chronic lymphocytic leukemia (CLL) usually involves the expansion of a clone CD5+ B cells synthesizing IgM antibodies. These appear to be blocked at antigen receptor-expressing stage cell differentiation and are thought not undergo an isotype class switch IgG or IgA production. In vivo in vitro studies suggest, however, that some instances terminal switching can occur. To test hypothesis occurs IgM+ B-type CLL cells, we analyzed PBMC 19 patients for presence transcripts encoding rearranged...
Reactive oxygen species (ROS) are metabolism by-products that may act as signaling molecules to sustain tumor growth. Antioxidants have been used impair cancer cell survival. Our goal was determine the mechanisms involved in response antioxidants of a human culture (PT4) containing glioblastoma (GBM) tumorigenic initiating cells (TICs). ROS production absence or presence N-acetyl-L-cysteine (NAC), tiron, and trolox evaluated by flow cytometry (FCM). The effects these on survival apoptosis...
The demonstration of EGFR T790M gene mutation in plasma is crucial to assess the eligibility Non Small Cell Lung Cancer (NSCLC) patients, who have acquired resistance first or second generation Tyrosine Kinase Inhibitors (TKIs), receive a subsequent treatment with osimertinib. Since circulating tumor DNA (ctDNA) present very low amounts plasma, high sensitive and specific methods are required for molecular analysis. Improving sensitivity detection ctDNA enables larger number NSCLC patients...
•This is a prospective, observational, multicenter, multidisciplinary, Italian study.•We investigate the feasibility of new diagnostic algorithm for LS.•A 'Lynch alert' in pathology report used as clear warning to clinicians.•We propose shift an oncologist-led mainstreaming genetic testing.•This study encourages establishment multidisciplinary workgroups. BackgroundInternational guidelines recommend universal screening Lynch syndrome (LS) through somatic DNA mismatch repair deficiency (dMMR)...