A5019847626- Prion Diseases and Protein Misfolding
- Neurological diseases and metabolism
- Trace Elements in Health
- Alzheimer's disease research and treatments
- Neuroscience and Neuropharmacology Research
- Chemokine receptors and signaling
- Pituitary Gland Disorders and Treatments
- Neuroendocrine Tumor Research Advances
- Cancer Cells and Metastasis
- Cancer, Hypoxia, and Metabolism
- interferon and immune responses
- Protein Tyrosine Phosphatases
- RNA regulation and disease
- Immunotherapy and Immune Responses
- Nitric Oxide and Endothelin Effects
- Neuroblastoma Research and Treatments
- Mitochondrial Function and Pathology
- Veterinary Oncology Research
- Angiogenesis and VEGF in Cancer
- Cancer Research and Treatments
- Virus-based gene therapy research
- Histone Deacetylase Inhibitors Research
- Amino Acid Enzymes and Metabolism
- Animal Genetics and Reproduction
- Caveolin-1 and cellular processes
University of Genoa
2012-2024
Ospedale Policlinico San Martino
2024
Washington University in St. Louis
2007
Pediatrics and Genetics
2003
Neuroscience Institute
1999
Tecnologie Avanzate (Italy)
1998-1999
Peptide receptors involved in pathophysiological processes represent promising therapeutic targets. Neuropeptide somatostatin (SST) is produced by specialized cells a large number of human organs and tissues. SST primarily acts as inhibitor endocrine exocrine secretion via the activation five G-protein-coupled receptors, named sst1–5, while central nervous system, neurotransmitter/neuromodulator, regulating locomotory cognitive functions. Critical points SST/SST receptor biology, such...
Somatostatin was reported to inhibit Kaposi's sarcoma (KS) cell (KS-Imm) xenografts through an antiangiogenic activity. Here, we show that somatostatin blocks growth of established KS-Imm tumors with the same efficacy as adriamycin, a clinically effective cytotoxic drug. Whereas cells do not express receptors (SSTRs), endothelial several SSTRs, in particular SSTR3. We investigated molecular mechanisms and receptor specificity inhibition angiogenesis. significantly inhibited angiogenesis vivo...
// Alessandro Corsaro 1 , Adriana Bajetto Stefano Thellung 1, 2 Giulia Begani Valentina Villa Mario Nizzari Alessandra Pattarozzi Agnese Solari Monica Gatti Aldo Pagano 3, 4 Roberto Würth Antonio Daga Federica Barbieri Tullio Florio Sezione di Farmacologia, Dipartimento Medicina Interna, University of Genova, Italy Centro Eccellenza per la Ricerca Biomedica (CEBR), 3 Department Experimental Medicine, IRCCS AOU San Martino - IST, Correspondence to: Florio, email: tullio.florio@unige.it...
Chloride intracellular channel-1 (CLIC1) activity controls glioblastoma proliferation. Metformin exerts antitumor effects in stem cells (GSCs) inhibiting CLIC1 activity, but its low potency hampers translation clinical settings.We synthesized a small library of novel biguanide-based compounds that were tested as antiproliferative agents for GSCs derived from human glioblastomas, vitro using 2D and 3D cultures vivo the zebrafish model. Compounds compared to metformin both efficacy inhibition...
Glioblastoma (GBM), one of the deadliest brain tumors, accounts for approximately 50% all primary malignant CNS therefore novel, highly effective remedies are urgently needed. Boron neutron capture therapy, which has recently repositioned as a promising strategy to treat high-grade gliomas, requires conspicuous accumulation boron atoms in cancer cells. With aim selectively deliver sodium borocaptate (BSH, 12 B atoms-including molecule already employed clinics) GBM cells, we developed novel...
Somatostatin and its analogs are active in the inhibition of SST receptor-positive endocrine neoplasms, but their activity mechanism nonendocrine tumors is not clear. potently inhibited growth a Kaposi's sarcoma xenograft nude mice, yet vitro tumor cells did express any known somatostatin receptors were by somatostatin. Histological examination revealed limited vascularization somatostatin-treated as compared with controls. was potent inhibitor angiogenesis an vivo assay. In vitro,...
Alzheimer disease (AD) is a heterogeneous neurodegenerative disorder characterized by (1) progressive loss of synapses and neurons, (2) intracellular neurofibrillary tangles, composed hyperphosphorylated Tau protein, (3) amyloid plaques. Genetically, AD linked to mutations in few proteins precursor protein (APP) presenilin 1 2 (PS1 PS2). The molecular mechanisms underlying neurodegeneration as well the physiological function APP are not yet known. A recent theory has proposed that PS1...
Cancer stem cells (CSCs) are considered the cell subpopulation responsible for breast cancer (BC) initiation, growth, and relapse. CSCs identified as self-renewing tumor-initiating cells, conferring resistance to chemo- radio-therapy several neoplasias. Nowadays, th (about 10mM)e pharmacological targeting of is an ineludible therapeutic goal. The antidiabetic drug metformin was reported suppress in vitro vivo CSC survival different tumors and, particular, BC preclinical models. However, few...
The prion diseases are transmissible neurodegenerative pathologies characterized by the accumulation of altered forms protein (PrP), termed PrPSc, in brain. Previous studies have shown that a synthetic peptide homologous to residues 106–126 PrP (PrP 106–126) maintains many characteristics i.e., ability form amyloid fibrils and induce apoptosis neurons. We investigated intracellular mechanisms involved cellular degeneration induced 106–126, using GH3 cells as model excitable cells. When...
Abstract Although growing evidence suggests that extracellular ATP might play roles in the control of astrocyte/neuron crosstalk CNS by acting on P2X 7 receptors, it is still unclear whether neuronal functions can be attributed to receptors. In present paper, we investigate location, pharmacological profile, and function receptors cerebrocortical nerve terminals freshly prepared from adult rats, measuring glutamate release calcium accumulation. The preparation chosen (purified synaptosomes)...
Glioblastoma multiforme is a highly aggressive brain tumor whose prognosis very poor. Due to early invasion of parenchyma, its complete surgical removal nearly impossible, and even after combined treatment (association surgery chemo- radio-therapy) five-year survival only about 10%. Natural products are sources novel compounds endowed with therapeutic properties in many human diseases, including cancer. Here, we report that the water extract Ruta graveolens L., commonly known as rue, induces...
Abstract According to the “gain-of-toxicity mechanism”, neuronal loss during cerebral proteinopathies is caused by accumulation of aggregation-prone conformers misfolded cellular proteins, although it still debated which aggregation state actually corresponds neurotoxic entity. Autophagy, originally described as a variant programmed cell death, now emerging crucial mechanism for survival in response variety stressors, including nutrient deprivation, damage cytoplasmic organelles, or...
Abstract Background Meningiomas are mainly benign brain tumors, although about 20% of histologically cases clinically aggressive and recur after resection. We hypothesize that meningioma invasiveness recurrence may be related to the presence cancer stem cells their high responsiveness CXCL12-CXCR4/CXCR7 chemokine axis. The aim this study was isolate from human samples, characterize them for biological features malignant behavior, identify role CXCR4/CXCR7 in these processes. Methods...
OBJECTIVE: Somatostatin is a powerful inhibitor of hormone secretion and cell proliferation. Treatment with somatostatin analogs in humans causes reduction size secretory activity some endocrine tumors, including somatotropic pituitary adenomas. Less studied are the effects agonists on non-functioning adenomas (NFPAs). In this study we characterized its analog lanreotide proliferation NFPAs vitro intracellular mechanisms involved. DESIGN: Twenty-three NFPA post-surgical specimens were...
Abstract: Release‐regulating 5‐hydroxytryptamine (5‐HT) autoreceptors in the rat brain belong to 5‐HT 1B subtype. On other hand, human seems lack receptors. In present work were characterized pharmacologically. Synaptosomes prepared from biopsy samples of neocortex labeled with [ 3 H]5‐HT and exposed superfusion selective receptor agonists antagonists during K + depolarization. The rank order potency as inhibitors overflow was > sumatriptan (5‐HT 1D/1B ) 8‐hydroxy‐2‐(di‐ n...