A5019925265- Lung Cancer Research Studies
- Lung Cancer Treatments and Mutations
- Alzheimer's disease research and treatments
- Cancer therapeutics and mechanisms
- Nitric Oxide and Endothelin Effects
- Neuroendocrine Tumor Research Advances
- Angiogenesis and VEGF in Cancer
- Neuroinflammation and Neurodegeneration Mechanisms
- Protein Kinase Regulation and GTPase Signaling
- Cell Adhesion Molecules Research
- Lung Cancer Diagnosis and Treatment
- Chronic Lymphocytic Leukemia Research
- Peptidase Inhibition and Analysis
- Monoclonal and Polyclonal Antibodies Research
- Histone Deacetylase Inhibitors Research
- RNA Interference and Gene Delivery
- Graphene and Nanomaterials Applications
- Regulation of Appetite and Obesity
- Cholinesterase and Neurodegenerative Diseases
- Neurological Disease Mechanisms and Treatments
- Phagocytosis and Immune Regulation
- Eicosanoids and Hypertension Pharmacology
- Animal health and immunology
- Prion Diseases and Protein Misfolding
- Reproductive System and Pregnancy
University of Campania "Luigi Vanvitelli"
2016-2025
Azienda Ospedaliera di Cosenza
2019
Northwestern University
2019
Ospedale SS. Annunziata
2019
Institute of Genetics and Biophysics
2012
Kaw Nation
2011
Capella University
2011
Istituto Neurologico Mediterraneo
2002-2009
Sapienza University of Rome
2001-2009
Istituto Superiore di Sanità
2009
Recent evidence suggests that besides its action on the central nervous system, leptin can modulate vascular tone through local mechanisms involving nitric oxide (NO) release. In this study, using a fluorescent probe for direct determination of NO, we demonstrated both in endothelial cells and vessels is able to stimulate NO The effect abolished by erbstatin A, Ca(2+)-independent tyrosine kinase inhibitor, whereas it not influenced calcium removal or other protein phosphorylation inhibitors,...
Hypertension affects nearly 20% of the population in Western countries and strongly increases risk for cardiovascular diseases. In pathogenesis hypertension, vasoactive peptide renin-angiotensin system, angiotensin II its G protein–coupled receptors (GPCRs), play a crucial role by eliciting reactive oxygen species (ROS) mediating vessel contractility. Here we show that mice lacking GPCR-activated phosphoinositide 3-kinase (PI3K)γ are protected from hypertension is induced administration...
Arterial hypertension is not only a major risk factor for cerebrovascular accidents, such as stroke and cerebral hemorrhage, but also associated to milder forms of brain injury. One the main causes neurodegeneration increase in reactive oxygen species (ROS) that common trait hypertensive conditions, thus suggesting mechanism could play role even onset hypertension-evoked To investigate this issue, we have explored effect acute-induced conditions on oxidative stress. aim, developed mouse...
Hypertension can lead to subarachnoid hemorrhage and eventually cerebral vasospasm. It has been suggested that the latter could be result of oxidative stress an inflammatory response evoked by hemorrhage. Because unavoidable consequence is lysis red blood cells, we first tested hypothesis on carotid arteries proinflammatory cytokine tumor necrosis factor-α contributes vascular hemolysis. We observed hemolysis induces a significant increase in both tissues, where it provokes Rac-1/NADPH...
Alzheimer's disease (AD) has been recently associated with vascular risk factors. β-amyloid peptides (AβP), the main component of senile plaques typical AD, circulate in soluble globular form bloodstream. Interestingly, AβP is able to induce endothelial dysfunction, and this effect may represent link between neuronal pathophysiological factors involved AD. We aimed clarify molecular mechanisms underlying AβP-induced toxicity. Using several methodological approaches, we have observed that...
Nebivolol is a beta1-adrenergic receptor antagonist that also reduces blood pressure by evoking endothelial NO production and vasodilation. We aimed at assessing whether nebivolol induces in the heart delineating molecular mechanisms involved. Using fluorescent probe diaminofluorescein, we found dose-dependent heart, statistically significant already 10(-7) mol/L. It not an effect because of blockade receptor, this shared another drug same class, atenolol. Because has been reported to act as...
Since vascular dysfunction is a main trait of obese subjects, in the present study we evaluated impact resistin, recently discovered hormone markedly increased obesity.We performed our analysis on aortic and mesenteric segments from young old C57BL/6 mice cultured endothelial cells. Resistin-induced effect was vitro vivo. Molecular analyses were by immunoprecipitation Western blotting.Recombinant murine resistin did not induce changes either basal tone or phenylephrine-induced contraction....
High blood pressure induces a mechanical stress on vascular walls and evokes oxidative dysfunction. The aim of this study was to characterize the intracellular signaling causing in response pressure. In carotid arteries subjected high levels, we observed not only an impaired vasorelaxation, increased superoxide production, NADPH oxidase activity, but also concomitant activation Rac-1, small G protein. Selective inhibition with adenovirus carrying dominant-negative Rac-1 mutant, significantly...
Glioblastoma multiforme is a highly aggressive brain tumor whose prognosis very poor. Due to early invasion of parenchyma, its complete surgical removal nearly impossible, and even after combined treatment (association surgery chemo- radio-therapy) five-year survival only about 10%. Natural products are sources novel compounds endowed with therapeutic properties in many human diseases, including cancer. Here, we report that the water extract Ruta graveolens L., commonly known as rue, induces...
Glioblastoma (GBM), a high-grade glioma (WHO grade IV), is the most aggressive form of brain cancer. Available treatment options for GBM involve combination surgery, radiation and chemotherapy but result in poor survival outcome. high-vascularized tumor antiangiogenic drugs are widely used therapy as adjuvants to control abnormal vasculature. Vasculogenic mimicry occurs an alternative vascularization mechanism, providing means whereby can escape anti-angiogenic therapies. Here, using vitro...
Neurofibromatosis type 1 (NF1) is one of the most common genetic tumor predisposition syndrome, caused by mutations in NF1. To date, few genotype-phenotype correlations have been discerned NF1, due to a highly variable clinical presentation. We aimed study molecular spectrum NF1 and monocentric cohort 85 patients (20 relatives, 65 sporadic cases). Clinical data were collected at time mutation analysis reviewed for accuracy this investigation. An internal phenotypic categorization was...
PURPOSE: Platinum-based chemotherapy currently represents standard treatment for advanced non–small-cell lung cancer. Gemcitabine is one of the most interesting agents in use cancer, and high response rates have been reported when it administered combination with cisplatin. The aim present study was to evaluate gemcitabine carboplatin a phase I-II study. PATIENTS AND METHODS: Chemotherapy-naive patients stage IIIB-IV cancer received at area under concentration-time curve (AUC) 5 mg/mL/min an...
Diabetes mellitus is a main risk factor for vascular diseases. Vascular injury induced by diabetes characterized endothelial dysfunction attributable to an increased oxidative stress. So far, the molecular mechanisms involved in vasculotoxic effects of are only partially known. We examined effect on stress and Rac-1 activation, small G-protein activation NADPH oxidase. Our results show that vessels different murine models cells treated with high glucose associated Rac-1/PAK binding...
Angiogenesis is a phenomenon that includes different processes, such as endothelial cell proliferation, differentiation, and migration, lead to the formation of new blood vessels involve several signal transduction pathways. Here we show tube assay simple in vitro method evaluate impact natural products on angiogenesis investigate molecular mechanisms involved. In particular, presence water extract Ruta graveolens (RGWE), cells are no longer able form cell-cell network RGWE effects human...
Insulin-like growth factor I (IGF-I) can be considered a potentially involved in arterial hypertension not only for its growth-promoting features but also effects on vascular tone. Nevertheless, the actions of hormone reactivity are still unexplored hypertension. Therefore, vasodilation induced by increasing doses IGF-I and modulation norepinephrine vasoconstriction low levels were tested aortic rings spontaneously hypertensive normotensive rats. The results indicate that evoked is impaired...
Neo-angiogenesis is a complex phenomenon modulated by the concerted action of several molecular factors. We have generated congenic line knockout mice carrying null mutations both placental growth factor (PlGF) and endothelial nitric oxide synthase (eNOS), two genes that play pivotal role in regulation pathological angiogenesis. In present study, we describe phenotype this new experimental animal model after surgically induced hind-limb ischemia. Plgf-/-, eNos-/-, Plgf-/- wild-type C57BL/6J...
<title>Abstract</title> In the last few years, probiotics have gained much attention within medical, pharmaceutical, and food fields, given health benefits provided by their consumption. They include several lactic acid bacteria (LAB) species, mostly belonging to genera <italic>Lactobacillus</italic>, <italic>Lactococcus</italic>, <italic>Streptococcus</italic>. Postbiotics are bioactive compounds (organic acids, short-chain fatty enzymes, neurotransmitters) produced bacterial fermentation...
Nebivolol is a selective beta(1)-adrenergic receptor antagonist that causes direct vasodilator effect attributed to the action on vascular nitric oxide (NO). This study aimed investigate whether nebivolol or its metabolites induces NO production and explore mechanisms underlying this pharmacologic effect.Conductance resistance arteries from Wistar-Kyoto rats (WKY) (n = 33) incubated with fluorescent probe diaminofluorescein-2 (DAF-2) were stimulated increasing concentrations of enantiomers...