A5084425284- Molecular Biology Techniques and Applications
- Genetic factors in colorectal cancer
- Immunodeficiency and Autoimmune Disorders
- Colorectal Cancer Treatments and Studies
- Chronic Lymphocytic Leukemia Research
- Cancer Genomics and Diagnostics
- Galectins and Cancer Biology
- HER2/EGFR in Cancer Research
- AI in cancer detection
- Ovarian cancer diagnosis and treatment
- Orthopaedic implants and arthroplasty
- Sarcoma Diagnosis and Treatment
- Reproductive System and Pregnancy
- Bone Metabolism and Diseases
- Appendicitis Diagnosis and Management
- Multiple and Secondary Primary Cancers
- Head and Neck Anomalies
- Thyroid Cancer Diagnosis and Treatment
- Occupational and environmental lung diseases
- Foreign Body Medical Cases
- Prostate Cancer Diagnosis and Treatment
- Mathematical Biology Tumor Growth
- dental development and anomalies
- Intraperitoneal and Appendiceal Malignancies
- Prostate Cancer Treatment and Research
University of Genoa
2015-2024
Ospedale Policlinico San Martino
2015-2024
Few studies have focused on antigen preservation in formalin-fixed and paraffin-embedded (FFPE) tissue old archival material additional are required, especially considering that these samples an irreplaceable resource for scientific clinical research. The purpose of this study is to verify FFPE stored several decades. From the pathology archives, blocks were selected dating back 1960s, 1970s, 1980s, 1990s, 2000s 2010. A panel 12 antibodies was applied immunoreactivities compared. While...
Abstract Background SMC1A is a subunit of the cohesin complex that participates in many DNA- and chromosome-related biological processes. Previous studies have established involved cancer development particular, overexpressed chromosomally unstable human colorectal (CRC). This study aimed to investigate whether could serve as therapeutic target for CRC. Methods At first, we studied effects either overexpression or knockdown vitro. Next, outcome knocking down (alone combination with...
The Lynch syndrome (LS) screening algorithm requires BRAF testing as a fundamental step to distinguish sporadic from LS-associated colorectal carcinomas (CRC). by immunohistochemistry (IHC) has shown variable results in the literature. Our aim was analyse concordance between
Identifying innovative molecules involved in the tumor immune escape process could help refine survival stratification of colorectal cancer (CRC) patients. HLA-G, a non-classical HLA molecule, physiologically tolerogenic mechanisms, has recently emerged as relevant prognostic marker other types, but ambiguous data are reported CRC setting. This study aims to evaluate HLA-G expression and potential series stage II/III CRCs. was evaluated 100 pT3 cases by means immunohistochemistry using 4H84...
Chronic lymphocytic leukemia (CLL) cells express the interleukin-23 receptor (IL-23R) chain, but expression of complementary IL-12Rβ1 chain requires cell stimulation via surface CD40 molecules (and not B-cell [BCR]). This induces a heterodimeric functional IL-23R complex and secretion IL-23, initiating an autocrine loop that drives leukemic expansion. Based on observation in 224 untreated Binet stage A patients cases with lowest miR-146b-5p concentrations had shortest time to first treatment...
Pathology archives are a treasure trove of paraffin embedded tissue spanning many years and covering wide variety tissues diseases. The possibility using old archival formalin fixed (FFPE) for diagnostic updates research projects is widespread need it requires stable, well-preserved samples. Immunohistochemistry performed on blocks may give unreliable results, in particular some antigens, such as Ki67. In consideration this phenomenon, our aim to comprehensively test identify methods which...
Mismatch repair/microsatellite instability (MMR/MSI) status in colorectal cancer (CRC) has become fundamental as a diagnostic, prognostic, and predictive factor. MMR immunohistochemistry (IHC) is considered simple reliable approach; however, its effectiveness depends on pre-analytic factors. Aim of this study was to investigate the impact different fixation times/protocols protein IHC quality. Left over tissue from surgically resected CRC samples (cold ischemia time < 30 min) where fixed...
Bone skeletal alterations are no longer considered a rare event in chronic lymphocytic leukemia (CLL), especially at more advanced stages of the disease. This study is aimed elucidating mechanisms underlying this phenomenon. marrow stromal cells, induced to differentiate toward osteoblasts osteogenic medium, appeared unable complete their maturation upon co-culture with CLL CLL-cell-derived conditioned media (CLL-cm) or CLL-sera (CLL-sr). Inhibition osteoblast differentiation was documented...
Summary Background The microscopic assessment of squamous epithelium lesions in gastro‐oesophageal reflux disease (GERD) is subjective. Ki67 nuclear antigen expressed by proliferating cells provides an objective measure regeneration the epithelium. Aim To evaluate expression GERD patients and controls, comparison with histological lesions, pH‐metry endoscopic data. Methods Eighty‐seven symptoms 20 symptom‐free controls underwent endoscopy 24‐h pH monitoring. Oesophageal biopsies (4 cm, 2 cm...
Multiple primary colorectal carcinomas (CRCs) synchronous, when detected approximately at the same time, or metachronous, a significant amount of time has elapsed between diagnoses can show both mismatch repair (MMR) status concordance and discordance tumors. The aim was to evaluate MMR monoinstitutional, retrospective cohort synchronous metachronous CRCs, with focus on frequency cases discordant status, explore metastatic nodal deposits. All CRCs diagnosed in our institution 2011 2023 were...
Abstract High‐grade gliomas (HGGs; WHO grades III and IV) are invariably lethal brain tumors. Low‐dose hyper‐radiosensitivity (HRS) of HGG is a well‐established phenomenon in vitro . However, possibly linked to the unavailability accurate animal models diseases, this therapeutic effect could not be consistently translated setting, thus impairing its subsequent clinical development. The purpose study was develop radiotherapeutic (RT) schedules permitting significantly improve overall survival...
Significant skeletal alterations are present in Chronic Lymphocytic Leukemia (CLL) patients; bone erosion, particularly evident the long shaft, appeared increased progressive disease stage. Moreover, partial colonization of with reactive marrow we documented via PET-FDG imaging suggests that neoplastic cell overgrowth contributes to derangement. Indeed, cytokines released by leukemic B cells impair osteoblast differentiation and enhance osteoclast formation vitro. CD16, Fcγ-RIIIa, has been...
This study aims to confirm the diagnostic accuracy of extra-prostatic extension (EPE) grading system and explore predictive capabilities prostate MRI while considering various features such as lesion location, apparent diffusion coefficient (ADC) values capsular enhancement sign (CES).
Introduction: Seeds may be found in gastrointestinal tissue samples, and their multifaceted appearance challenging. The aim is to report a rough incidence of pathology samples which show seeds, specify the most frequent sample types an iconography commonly identified seeds. Materials Methods: Between 2017 2020, all cases seeds/seed parts were found, collected seed type described by referencing image library. Results: Fifty with complete collected: 16 colonic resections for colorectal cancer...
Arginine metabolism plays a significant role in regulating cell function, affecting tumor growth and metastatization. To study the effect of arginine-catabolizing enzyme Arginase1 (ARG1) on microenvironment, we generated mouse model mammary carcinogenesis by crossbreeding transgenic line overexpressing ARG1 macrophages (FVBArg+/+) with MMTV-Neu (FVBNeu+/+). This double (FVBArg+/-;Neu+/+) showed shortening latency, an increase number nodules. Transfer cells from FVBNeu+/+ into either FVB wild...