A5015893412- Bone Tissue Engineering Materials
- Osteoarthritis Treatment and Mechanisms
- 3D Printing in Biomedical Research
- PARP inhibition in cancer therapy
- Chronic Lymphocytic Leukemia Research
- Periodontal Regeneration and Treatments
- DNA Repair Mechanisms
- Silk-based biomaterials and applications
- Immunodeficiency and Autoimmune Disorders
- Knee injuries and reconstruction techniques
- Electrospun Nanofibers in Biomedical Applications
- Mesenchymal stem cell research
- Cancer therapeutics and mechanisms
- Orthopaedic implants and arthroplasty
- Phagocytosis and Immune Regulation
- Cell Adhesion Molecules Research
- Neuroblastoma Research and Treatments
- Graphene and Nanomaterials Applications
- Bone Metabolism and Diseases
- Peroxisome Proliferator-Activated Receptors
- Tissue Engineering and Regenerative Medicine
- Inflammatory mediators and NSAID effects
- Neutropenia and Cancer Infections
- PI3K/AKT/mTOR signaling in cancer
- Trace Elements in Health
University of Genoa
2016-2025
Institute of Electronics, Computer and Telecommunication Engineering
2024
Istituto Nazionale di Fisica Nucleare, Sezione di Genova
2014-2016
Stem Cell Institute
2016
Technology Centre Prague
2016
Tecnologie Avanzate (Italy)
2002-2012
Biotechnology Research Center
2008-2011
Catholic Biblical Association
2011
Genoa (Brazil)
2011
Universidade do Porto
2009
Studying cartilage differentiation, we observed the emergence of inflammation-related proteins suggesting that a common pathway was activated in differentiation and inflammation. In present paper, investigated expression enzyme Cyclooxygenase-2 (COX-2) during inflammatory response chondrocytic cell line MC615. Cells were cultured either as (i) proliferating prechondrogenic cells expressing type I collagen or (ii) differentiated hyperconfluent Sox9 II collagen. The p38 NF-kB pathways standard...
Physical forces are known to influence the synthesis, assembly and degradation of cartilage extracellular matrix. The expression oligomeric matrix protein (COMP) was found be sensitive long term cyclic compression. Explants calf articular as well cylindrical alginate/chondrocyte constructs were subjected uniaxial unconfined dynamic compression for 18 hours after which total mRNA extracted from samples. COMP assessed by means semi-quantitative RT-PCR Northern blot techniques. transcript...
Bone repair was addressed in a critical-sized defect model sheep, combining ceramic biomaterial and mesenchymal progenitor cells. The defects the tibial mid-diaphysis were treated with autologous bone or silicon-stabilized tricalcium phosphate biomaterial, implemented not by addition of expanded marrow stromal An internal locking compression plate an external fixator applied for stabilization. Radiographies taken during 8 months follow-up: pixel grey levels lesion areas determined to...
In modern biomaterial design the generation of an environment mimicking some extracellular matrix features is envisaged to support molecular cross-talk between cells and scaffolds during tissue formation/remodeling. bone substitutes chemical biomimesis has been particularly exploited; conversely, relevance pre-determined scaffold architecture for regenerated outputs still unclear. Thus we aimed demonstrate that a different organization collagen fibers within newly formed under unloading...
In tissue-engineered applications bone marrow stromal cells (BMSCs) are combined with scaffolds to target regeneration; animal models have been devised and the cells' long-term engraftment has widely studied. However, in regenerated bone, cell number is severely reduced respect initially seeded BMSCs. This reflects natural low cellularity of but underlines selectivity differentiation processes. this respect, we evaluated short-term survival BMSCs, transduced luciferase gene, after...
Neuroblastoma (NB) is a solid tumor occurring in infancy and childhood, whose high-risk form has currently survival rate < 50 %, despite aggressive treatments. Additionally, drugs-induced secondary tumorigenesis the emergency of drug resistance worsen already worrying scenario, calling for an urgent development new extra-genomic Quaternary phosphonium salts (QPSs) possess both antimicrobial anticancer effects. Triphenyl (TPPs) are mitochondria-targeting compounds, exerting...
Minimally invasive medical treatments for peripheral nerve stimulation are critically needed to minimize surgical risks, enhance the precision of therapeutic interventions, and reduce patient recovery time. Magnetoelectric nanoparticles (MENPs), known their unique ability respond both magnetic electric fields, offer promising potential medicine due dual tunable functionality. In this study a multi-physics modeling MENPs was performed, assessing capability be targeted through external fields...
Autologous chondrocyte implantation is currently applied in clinics as an innovative tool for articular cartilage repair. Animal models have been and still are being used to validate further improve the technique. However, various species, outcome varies from hyaline-like fibrocartilage. This may be due partly spontaneous dedifferentiation of chondrocytes once cultured vitro. Here we assessed whether extent between species hypothesized that level phenotype stability during expansion...
Cell-based cartilage resurfacing requires ex vivo expansion of autologous articular chondrocytes. Defined culture conditions minimize expansion-dependent phenotypic alterations but maintenance the cells' differentiation potential must be carefully assessed. Transforming growth factor β-1 (TGF β-1) positively regulates expression several proteins, its therapeutic application in damaged is controversial. Thus we evaluated outcomes cultured human chondrocytes exposed to TGF during monolayer a...
Hepatocyte growth factor, produced by stromal and follicular dendritic cells, present at high concentrations in the sera of patients with chronic lymphocytic leukemia, prolongs survival leukemic B cells interacting their receptor, c-MET. It is, however, unknown whether hepatocyte factor influences microenvironmental such as nurse-like which deliver signals to clone. We evaluated expression c-MET on monocytes from leukemia searched for phenotypic/functional features supposed be influenced...
One of the current major challenges in orthopedic surgery is treatment meniscal lesions. Some main issues include mechanical consistency implants, besides their fixation methods and integration with host tissues. To tackle these aspects we realized a micro-porous, gelatin/polyvinyl alcohol (PVA)-based hydrogel to approach high percentage water present native tissue, recapitulating its biomechanical features, and, at same time, realizing porous implant, permissive cell infiltration tissue...
Abstract Background Bone metastases are a common and dismal consequence of lung cancer that is leading cause death. The role IL-7 in promoting bone has been previously investigated NSCLC, but many aspects remain to be disclosed. To further study function metastasis, we developed human-in-mice model aggression by NSCLC analyzed human metastasis biopsies. Methods We used NOD/SCID mice implanted with bone. After engraftment, two groups were injected subcutaneously A549, cell line, either close...
Background Chronic lymphocytic leukemia cells are characterized by an apparent longevity in vivo which is lost when they cultured vitro. Cellular interactions and factors provided the microenvironment appear essential to cell survival may protect leukemic from cytotoxicity of conventional therapies. Understanding cross-talk between stroma interest for identifying signals supporting disease progression developing novel therapeutic strategies.Design Methods Different types, sharing a common...
Treatment of full-thickness cartilage defects relies on osteochondral bilayer grafts, which mimic the microenvironment and structure two affected tissues: articular subchondral bone. However, integrity stability grafts are hampered by presence a weak interphase, generated layering processes scaffold manufacturing. We describe here design development monolithic graft, avoiding delamination distinct layers but preserving cues for selective generation A highly porous polycaprolactone-based...
In any therapeutic model involving a tissue-engineering approach to the repair of partial-thickness articular cartilage defects, chondrogenic differentiation factor is required ensure tissue-specific healing. Transforming growth factor-beta1 (TGF-beta1) known act in this capacity, but at such high concentrations as render its direct injection into joint cavity inadvisable. This situation calls for delivery system that can be applied directly defect site and will release drug gradually over...
Graphene-derived materials, such as graphene oxide (GO), have been widely explored for biomedical and biological applications, including cancer research. Despite some recent works proving that GO inhibits the migration invasion of different cells, so far most these in vitro studies conducted using sheets dispersed solution or a planar film. On contrary, little is known about cellular activities, cell viability, adhesion, spreading, when cells interface with functionalized hydrogel-based...