A5042679651- Veterinary Oncology Research
- Virus-based gene therapy research
- Sarcoma Diagnosis and Treatment
- Infectious Diseases and Mycology
- Immunotherapy and Immune Responses
- Oral and Maxillofacial Pathology
- Salivary Gland Tumors Diagnosis and Treatment
- Bone Tumor Diagnosis and Treatments
- Microbial infections and disease research
- Cancer Research and Treatments
- Tumors and Oncological Cases
- Cancer Genomics and Diagnostics
- Mast cells and histamine
- vaccines and immunoinformatics approaches
- Cell Adhesion Molecules Research
- Cancer, Hypoxia, and Metabolism
- Veterinary Orthopedics and Neurology
- Hernia repair and management
- Veterinary Medicine and Surgery
- Urological Disorders and Treatments
- interferon and immune responses
- Peptidase Inhibition and Analysis
- Fibroblast Growth Factor Research
- Angiogenesis and VEGF in Cancer
- Protease and Inhibitor Mechanisms
University of Turin
2016-2025
A. O. Ordine Mauriziano di Torino
2022
University of Bologna
2020
University of Padua
2011
Istituto Zooprofilattico Sperimentale delle Venezie
2011
University of Milan
2008
Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna "Bruno Ubertini"
2008
University of Parma
2005
AULSS 2 Marca Trevigiana
2005
Torino e-district
2002
Due to the many similarities with its human counterpart, canine malignant melanoma (cMM) is a valuable model in which assess efficacy of novel therapeutic strategies. The herein used evaluate immunogenicity, safety, and chondroitin sulfate proteoglycan-4 (hCSPG4) DNA-based vaccine. fact that homology between hCSPG4 cCSPG4 amino-acidic sequences stands at more than 80% provides rationale for using an DNA vaccine cMM model.Dogs stage II-III surgically resected CSPG4-positive oral MM were...
Lymph node (LN) metastasis in canine cutaneous mast cell tumours (cMCTs) is a well-known negative prognostic factor. The role of lymphadenectomy the treatment stage II disease remains controversial because its uncertain therapeutic benefit. Aim this retrospective study was to investigate impact on tumour control and survival for dogs with cMCTs. Dogs firstly occurring, histologically confirmed cMCT LN undergoing resection primary medical thereafter were retrospectively enrolled. classified...
Objective— To evaluate recurrence rate and disease‐free interval (DFI) of dogs with low‐grade soft tissue spindle cell sarcoma the extremities treated by marginal excision. Study Design— Retrospective study. Animals— Dogs (n=35) sarcoma. Methods— Medical records were reviewed that had surgical resection at or distal to elbow stifle included. Results— Histopathologic margins dirty (12 dogs), clean but close (12), (11). Follow‐up after surgery occurred from 210 2202 days (minimum, 180 days)....
The expression of Ki67, BCL-2, and COX-2 was investigated in 53 canine cutaneous mast cell tumors (MCTs) by immunohistochemistry quantitative real time polymerase chain reaction (qPCR) to evaluate their prognostic significance the association with histologic grading mitotic index (MI). MCTs were graded according Patnaik system novel 2-tier proposed Kiupel. numbers figures/10 high-power fields (MI) counted. Both systems significantly associated prognosis. limited value for grade 2 MCTs, 23%...
Reported post-surgery 1-year survival rate for oral canine malignant melanoma (cMM) is around 30%; novel treatments are needed as the role of adjuvant chemotherapy unclear. This prospective study regards electrovaccination with human chondroitin sulfate proteoglycan-4 (hCSPG4)-encoded plasmid in 23 dogs resected II/III-staged CSPG4-positive cMM compared 19 only cMM. Vaccination resulted 6-, 12-, 18- and 24-month 95.6, 73.9, 47.8 30.4%, respectively [median time (MST) 684 days, range 78-1694,...
Malignant canine mammary tumors represent 50% of all neoplasms in female dogs. Matrix metalloproteinases (MMPs) and tissue inhibitors (TIMPs) are thought to be involved tumor progression, they also associated with the reactive stroma, which provides structural vascular support for growth. MMP-2, MMP-9 MT1-MMP were expressed at both mRNA protein levels samples. MMP-2 immunohistochemical reactions evident epithelial cells stromal compartment varying degrees; particular, intensity staining was...
ABSTRACT Canine oral melanoma (OM) exhibits poor prognosis and limited treatment options. The success of immune checkpoint inhibitors (ICIs) in human has driven interest similar therapeutic approaches the dog, although immunosuppressive mechanisms adopted by canine OM remain unclear. This study aimed to evaluate expression checkpoints PD‐1/PD‐L1 CTLA‐4 RNAscope situ hybridization (ISH) OM, investigate their pattern explore potential role progression. Twenty‐four formalin‐fixed,...
Hepatoid perianal gland tumors are relatively common in dogs, accounting for 25% of all skin tumors. However, the specific factors involved their development still not completely clear. It has been established that hormonal influences can impact formation these The prognosis dogs with depends largely on histology (benign vs. malignant) and, case malignancy, it suggested stage disease is important, a more favorable outcome having small (under 5 cm diameter), non-metastatic adenocarcinomas...
Gastric dilatation-volvulus (GDV) syndrome is a life-threatening emergency and its physiopathology treatment have been studied for decades. Despite ongoing research, the mortality rate still high. The aims of this study are to describe outcome GDV patients treated from 2011 2024 at veterinary teaching hospital Grugliasco (Turin, Italy); analyze risk prognostic factors, comparing obtained data with current literature; evaluate how patients' management has changed over years. included 130 dogs...
Canine oral malignant melanoma (OMM) is an aggressive, spontaneously occurring tumor carrying a poor to guarded prognosis and relatively limited therapeutic strategies. In this landscape, chondroitin sulfate proteoglycan (CSPG)4 represents promising immunotherapeutic target. The objective of bi-center prospective study was examine the clinical outcome OMM-bearing dogs treated with surgery adjuvant electroporation using DNA vaccine (HuDo-CSPG4) encoding both human (Hu) canine (Do) portions...
Abstract Objective The first aim was to describe the incidence and severity of surgical complications following peripheral lymphadenectomy in dogs. second compare three techniques: unassisted lymphadenectomy, intraoperative guidance by methylene blue dye alone (MB) or a combination γ‐probe MB (γ‐MB). third assess whether number, palpability, site lymph nodes (LNs) influenced complications. Study design Retrospective multicenter study. Sample population Lymphadenectomies ( n = 201) from 163...
Abstract Objective To evaluate the recovery outcomes of cats following limb amputation for appendicular bone tumors. Methods This retrospective, multi-institutional study included that underwent thoracic or pelvic to treat primary tumors (2006 2019). Short- and long-term postoperative complications were investigated. Owners surveyed their perceptions satisfaction regarding adaptation outcomes. Fisher exact tests used compare results between different levels performed. Results A total 68...
Cutaneous mast cell tumors (MCTs) are among the most common neoplasms in dogs and show a highly variable biologic behavior. Histological grading, proliferation markers, KIT immunohistochemistry typically used to predict post-surgical prognosis. In present study, c-KIT messenger RNA (mRNA) expression was measured canine MCTs its relationship with tumor grade, immunohistochemical staining pattern, prognosis, mutations investigated. A significant increase of mRNA observed versus healthy skin...
Canine malignant melanoma (CMM) is the most common canine oral tumour, and up to 70-75% of dogs in stage II-III die within 1 year after surgery. The purpose this study was evaluate expression platelet-derived growth factors receptors (PDGFR)-α -β II III CMMs correlate it with prognosis. PDGFRs evaluated by immunohistochemistry on 48 cases formalin-fixed CMM samples correlated clinical-pathological findings outcome co-expression observed 37.5% cases. Positivity for PDGFR-α receptor present...
Canine oral malignant melanoma is locally invasive and highly metastatic. At present, the best option for local control en bloc excision followed by radiation if margins are incomplete. Adjuvantly, role of chemotherapy dubious while immunotherapy appears encouraging. This retrospective study evaluated 155 dogs with melanomas (24 stage I, 54 II, 66 III 11 IV) managed in a single institution. The aim was to evaluate differences median survival time (MST) disease-free interval (DFI) between...
Background Melanoma is the most lethal form of skin cancer in humans. Conventional therapies have limited efficacy, and overall response still unsatisfactory considering that immune checkpoint inhibitors induce lasting clinical responses only a low percentage patients. This has prompted us to develop vaccination strategy employing tumor antigen chondroitin sulfate proteoglycan (CSPG)4 as target. Methods To overcome host’s unresponsiveness self-antigen CSPG4, we taken advantage conservation...
The high mortality rate of osteosarcoma (OSA) patients highlights the requirement alternative strategies. young age patients, as well rarity and aggressiveness disease, limits opportunities for robust testing novel therapies, suggesting need valuable preclinical systems. Having previously shown overexpression chondroitin sulfate proteoglycan (CSPG)4 in OSA, herein functional consequences its downmodulation human OSA cells were evaluated vitro, with a significant impairment cell...