A5077942479- Immunotherapy and Immune Responses
- Cancer Research and Treatments
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Monoclonal and Polyclonal Antibodies Research
- Extracellular vesicles in disease
- Virus-based gene therapy research
- RNA modifications and cancer
- Single-cell and spatial transcriptomics
- Cancer Genomics and Diagnostics
- Cancer Cells and Metastasis
- Nanoplatforms for cancer theranostics
- RNA Research and Splicing
- Cell Image Analysis Techniques
- Cancer Immunotherapy and Biomarkers
- interferon and immune responses
- vaccines and immunoinformatics approaches
- Genomics and Phylogenetic Studies
- Immune Cell Function and Interaction
- Amyotrophic Lateral Sclerosis Research
- Lymphoma Diagnosis and Treatment
- Cell Adhesion Molecules Research
- Ovarian cancer diagnosis and treatment
- Colorectal Cancer Treatments and Studies
- Veterinary Oncology Research
University of Turin
2016-2025
Kantonsspital Baselland
1990
University of Basel
1990
Abstract Resistance to therapy and lack of curative treatments for metastatic breast cancer suggest that current therapies may be missing the subpopulation chemoresistant radioresistant stem cells (CSC). The ultimate success any treatment well rest on CSC eradication, but specific anti-CSC are still limited. A comparison transcriptional profiles murine Her2+ tumor TUBO their derived CSC-enriched tumorspheres has identified xCT, functional subunit cystine/glutamate antiporter system xc−, as a...
Mesenchymal stromal cells (MSCs) are adult, multipotent of mesodermal origin representing the progenitors all tissues. MSCs possess significant and broad immunomodulatory functions affecting both adaptive innate immune responses once primed by inflammatory microenvironment. Recently, role extracellular vesicles (EVs) in mediating therapeutic effects has been recognized. Nevertheless, molecular mechanisms responsible for properties MSC-derived EVs (MSC-EVs) still poorly characterized....
Cancer stem cells (CSCs) are responsible for tumor progression, metastases, resistance to therapy, and recurrence. Therefore, the identification of molecules involved in CSC self-renewal is a necessary step toward more effective therapies. To this aim, through transcription profiling murine ErbB2+ cell line TUBO vs. derived CSC-enriched mammospheres, Toll-like receptor 2 (TLR2) was identified as 2-fold overexpressed CSCs, confirmed by qPCR cytofluorimetric analysis. TLR2 signaling inhibition...
Alteration in RNA metabolism, concerning both coding and long non-coding RNAs (lncRNAs), may play an important role Amyotrophic Lateral Sclerosis (ALS) pathogenesis. In this work, we performed a whole transcriptome RNA-seq analysis to investigate the regulation of Sporadic ALS patients (SALS), mutated (FUS, TARDBP SOD1) matched controls Peripheral Blood Mononuclear Cells (PBMC). Selected transcripts were validated spinal cord tissues. A total 293 differentially expressed (DE) lncRNAs was...
Bladder cancer (BC) is the most frequent malignancy of urinary tract with a high incidence in men and smokers. Currently, there are no non-invasive markers useful for BC diagnosis subtypes classification that could overcome invasive procedures such as cystoscopy. Dysregulated miRNA profiles have been associated numerous cancers, including BC. Cell-free miRNAs abundantly present variety biofluids urine make them promising candidates biomarker discovery. In study, identification fingerprints...
Due to the many similarities with its human counterpart, canine malignant melanoma (cMM) is a valuable model in which assess efficacy of novel therapeutic strategies. The herein used evaluate immunogenicity, safety, and chondroitin sulfate proteoglycan-4 (hCSPG4) DNA-based vaccine. fact that homology between hCSPG4 cCSPG4 amino-acidic sequences stands at more than 80% provides rationale for using an DNA vaccine cMM model.Dogs stage II-III surgically resected CSPG4-positive oral MM were...
Reproducibility of a research is key element in the modern science and it mandatory for any industrial application. It represents ability replicating an experiment independently by location operator. Therefore, study can be considered reproducible only if all used data are available exploited computational analysis workflow clearly described. However, today reproducing complex bioinformatics analysis, raw list tools could not enough to guarantee reproducibility results obtained. Indeed,...
Identifying biomarkers is essential for early diagnosis of neurodegenerative diseases (NDs). Large (LEVs) and small extracellular vesicles (SEVs) are (EVs) different sizes biological functions transported in blood they may be valid NDs. The aim our study was to investigate common miRNA signatures plasma derived LEVs SEVs Alzheimer’s disease (AD), Parkinson’s (PD), Amyotrophic Lateral Sclerosis (ALS) Fronto-Temporal Dementia (FTD) patients. were isolated from patients healthy volunteers (CTR)...
In an attempt to reveal deregulated miRNAs associated with the progression of carcinomas developed in BALB-neuT transgenic mice, we found increased expression miR-135b during malignancy. Relevantly, observed that is up-regulated basal or normal-like human breast cancers, and it correlates patient survival early metastatization. Therefore, investigated its biological functions by modulating (up- down-regulation) mammary tumor cells. Although no effect was on proliferation cell culture...
The role of non-coding RNAs in different biological processes and diseases is continuously expanding. Next-generation sequencing together with the parallel improvement bioinformatics analyses allows accurate detection quantification an increasing number RNA species. With aim exploring new potential biomarkers for disease classification, a clear overview expression levels common/unique small species among biospecimens necessary. However, except miRNAs plasma, there are no substantial...
// Mariangela De Robertis 1, 2 , Maddalena Arigoni 3 Luisa Loiacono 4, 5 Federica Riccardo Raffaele Adolfo Calogero Yana Feodorova 6, 7 Dessislava Tashkova 8 Vesselin Belovejdov Victoria Sarafian Cavallo Emanuela Signori 1 Laboratory of Molecular Medicine and Biotechnology, Center Integrated Research, Campus Bio-Medico University Rome, 00128 Italy Pathology Experimental Oncology, Institute Translational Pharmacology, National Research Council (CNR), 00133 Department Biotechnology Health...
Abstract Single-cell RNA sequencing (scRNAseq) is an essential tool to investigate cellular heterogeneity. Thus, it would be of great interest being able disclose biological information belonging cell subpopulations, which can defined by clustering analysis scRNAseq data. In this manuscript, we report a that developed for the functional mining single clusters based on Sparsely-Connected Autoencoder (SCA). This allows uncovering hidden features associated with We implemented two new metrics,...
Objectives There is a lack of effective biomarkers for neurodegenerative diseases (NDs) such as Alzheimer's disease (AD), Parkinson's (PD), amyotrophic lateral sclerosis (ALS), and frontotemporal dementia. Extracellular vesicle (EV) RNA cargo can have an interesting potential non-invasive biomarker NDs. However, the knowledge about abundance EV-mRNAs their contribution to neurodegeneration not clear. Methods Large small EVs (LEVs SEVs) were isolated from plasma patients healthy volunteers...
Anaplastic lymphoma kinase (ALK) tyrosine inhibitors (TKIs) show potent efficacy in several ALK-driven tumors, but the development of resistance limits their long-term clinical impact. Although mechanisms have been studied extensively non–small cell lung cancer, they are poorly understood anaplastic large (ALCL). Here, we identify a survival pathway supported by tumor microenvironment that activates phosphatidylinositol 3-kinase γ (PI3K-γ) signaling through C-C motif chemokine receptor 7...
Additional copies of chromosome 1 long arm (1q) are frequently found in multiple myeloma (MM) and predict high-risk disease. Available data suggest a different outcome biology patients with amplification (Amp1q, ≥4 1q) vs. gain (Gain1q, 3 1q. We evaluated the impact Amp1q/Gain1q on newly diagnosed MM enrolled FORTE trial (NCT02203643). Among 400 available 1q data, 52 (13%) had Amp1q 129 (32%) Gain1q. After median follow-up 62 months, progression-free survival (PFS) was 21.2 months group,...
Abstract Background Malignant mesothelioma (MM) is a rare and aggressive form of cancer that affects the mesothelial surfaces, associated with exposure to asbestos fibres. To date, no cure available for MM therapeutically approved treatments are based on use platinum compounds often used in combination other drugs. We have previously analysed efficacy cisplatin/piroxicam (CDDP/P) combined treatment showing this was able reduce vivo tumor growth. Several studies reported platinum-drug...
The recombinant bovine somatotropin (rbST) is a synthetic hormone developed to mimic the effects of endogenous growth hormone, also known as (bST). Although rbST use in dairy cows authorized several countries, it currently banned Europe. Different methods for screening and confirmatory detection were developed, mainly based on LC-MS/MS immune-enzymatic assays. However, some commercial forms have above same amino acid sequence bST, making difficult produce reliable differentiation from forms....
Aside from its classical role in fighting infections, complement is an important, although poorly understood, component of the tumor microenvironment. In particular, growth-regulatory activities remain under debate. To assess system progression autochthonous mammary carcinomas, we have crossed 3 (C3)-deficient (C3−/−) BALB/c male mice with females expressing activated rat Her2/neu oncogene (neuT). Although neuT transgenic develop spontaneous cancers 100% penetrance, a significantly shorter...
Background Massive Parallel Sequencing methods (MPS) can extend and improve the knowledge obtained by conventional microarray technology, both for mRNAs short non-coding RNAs, e.g. miRNAs. The processing used to extract interpret information are an important aspect of dealing with vast amounts data generated from read sequencing. Although number computational tools MPS analysis is constantly growing, their strengths weaknesses as part a complex analytical pipe-line have not yet been well...
Non-small cell lung cancer (NSCLC) accounts for 81% of all cases and they are often fatal because 60% the patients diagnosed at an advanced stage. Besides need earlier diagnosis, there is a high additional effective therapies. In this work, we investigated feasibility progression mouse model, mimicking features human aggressive NSCLC, as biological reservoir potential therapeutic targets biomarkers. We performed RNA-seq profiling on total RNA extracted from lungs 30 week-old...
Angiomotin (Amot) is one of several identified angiostatin receptors expressed by the endothelia angiogenic tissues. We have shown that a DNA vaccine targeting Amot overcome immune tolerance and induce an antibody response hampers progression incipient tumors. Following our observation increased expression on tumor concomitant with from pre-neoplastic lesions to full-fledged carcinoma, we evaluated effect anti-Amot vaccination clinically evident Electroporation plasmid coding for human...