A5005050372- Cancer Immunotherapy and Biomarkers
- Lung Cancer Treatments and Mutations
- Cancer Cells and Metastasis
- Acute Myeloid Leukemia Research
- Acute Lymphoblastic Leukemia research
- Mesenchymal stem cell research
- Extracellular vesicles in disease
- Immune cells in cancer
- MicroRNA in disease regulation
- Hedgehog Signaling Pathway Studies
- Lung Cancer Research Studies
- Histone Deacetylase Inhibitors Research
- Cancer-related gene regulation
- Immune Cell Function and Interaction
- Chronic Myeloid Leukemia Treatments
- Epigenetics and DNA Methylation
- Cancer-related Molecular Pathways
- Wnt/β-catenin signaling in development and cancer
- Kruppel-like factors research
- Synthesis of Organic Compounds
- Genomics, phytochemicals, and oxidative stress
- Cell Adhesion Molecules Research
- Peptidase Inhibition and Analysis
- Retinoids in leukemia and cellular processes
- Cholangiocarcinoma and Gallbladder Cancer Studies
Université de Versailles Saint-Quentin-en-Yvelines
2018-2025
Université Paris-Saclay
2019-2025
Hôpital Ambroise-Paré
2020-2023
University of Verona
2013-2020
Académie de Versailles
2018
Abstract Mesenchymal stromal cells (MSCs) are multipotent cells, immunomodulatory stem that currently used for regenerative medicine and treatment of a number inflammatory diseases, thanks to their ability significantly influence tissue microenvironments through the secretion large variety soluble factors. Recently, several groups have reported presence extracellular vesicles (EVs) within MSC secretoma, showing beneficial effect in different animal models disease. Here, we standardized...
Master developmental pathways, such as Notch, Wnt, and Hedgehog, are signaling systems that control proliferation, cell death, motility, migration, stemness. These not only commonly activated in many solid tumors, where they drive or contribute to cancer initiation, but also primary metastatic tumor development. The reactivation of pathways stroma favors the development stem cells allows their maintenance, indicating these particularly attractive targets for efficient anticancer therapies,...
Mesenchymal stromal cells (MSCs) are adult, multipotent of mesodermal origin representing the progenitors all tissues. MSCs possess significant and broad immunomodulatory functions affecting both adaptive innate immune responses once primed by inflammatory microenvironment. Recently, role extracellular vesicles (EVs) in mediating therapeutic effects has been recognized. Nevertheless, molecular mechanisms responsible for properties MSC-derived EVs (MSC-EVs) still poorly characterized....
Both preclinical and clinical investigations suggest that Notch signalling is critical for the development of many cancers their response to chemotherapy. We previously showed inhibition abrogates stromal-induced chemoresistance in lymphoid neoplasms. However, role acute myeloid leukemia (AML) its contribution crosstalk between cells bone marrow stromal remain controversial. Thus, we evaluated pathway proliferation, survival AML co-culture with mesenchymal expanded from both healthy donors...
Mesenchymal stromal cells (MSCs) are adult multipotent stem residing as pericytes in various tissues and organs where they can differentiate into specialized to replace dying damaged tissues. These commonly found at injury sites tumors that known behave like " wounds do not heal." In this article, we discuss the mechanisms of MSCs migrating, homing, repairing injured We also review a number reports showing tumor microenvironment triggers plasticity induce malignant neoplastic tissue...
Abstract Graft-vs-host-disease (GvHD) is currently the main complication of allogeneic hematopoietic stem cell transplantation. Mortality and morbidity rates are particularly high, especially in steroid-refractory acute GvHD (aGvHD). Immune regulatory human bone marrow mesenchymal stromal cells (hMB-MSCs) represent a therapeutic approach to address this issue. Unfortunately, their effect hardly predictable vivo due several variables, that is, MSC tissue origin, concentration, dose number,...
Most of advanced non-small cell lung cancer (NSCLC) patients will experience tumor progression with immunotherapy (IO). Preliminary data suggested an association between high plasma HGF levels and poor response to IO in NSCLC. Our study aimed evaluate further the role HGF/MET pathway resistance We included retrospectively 82 consecutive NSCLC from two academic hospitals. Among them, 49 received ICIs alone or combination chemotherapy (CT), while 33 as control group. analyzed by ELISA...
In this study, we compared the overall gene and pathway expression profiles of HS-5 HS-27A stromal cell lines with those primary bone marrow MSCs to verify if they can be considered a reliable alternative tool for evaluating contribution in tumor development immunomodulation. Indeed, due their easier manipulation vitro as MSC cultures, several published studies took advantage assess biological mechanisms mediated by cells influencing biology immune responses. However, process carried out...
Solid epidemiological evidences connect obesity with incidence, stage and survival in pancreatic cancer. However, the underlying mechanistic basis linking adipocytes to cancer progression remain largely elusive. We hypothesized that factors secreted by could be responsible for epithelial-to-mesenchymal transition (EMT) induction and, turn, a more aggressive phenotype models of preneoplastic lesions.We studied role two adipogenic model systems from primary human bone marrow stromal cells...
Abstract Notch3 and Notch4 support survival of primary B-cell acute lymphoblastic leukemia (B-ALL) cells, suggesting a role for Notch signaling in drug response. Here we used vitro, silico, vivo mouse xenograft model-based approaches to define the pathway B-ALL chemosensitivity. We observed significant receptor ligand expression cells cell lines. Primary from high-risk patients overexpressed Notch3, Notch4, Jagged2 while displaying reduction levels Notch1-4 following chemotherapy. then...
Background Despite prolonged tumor response to immune checkpoint inhibitors (ICIs) for a subset of patients with advanced non-small cell lung cancer (NSCLC), secondary resistance will occur majority these patients. The understanding late progression mechanisms ICIs is important improve future treatment strategies. Methods We performed whole-exome sequencing (WES) on circulating DNA and compared molecular profiles between the beginning ICI in NSCLC treated who had initial progression, after...
Hedgehog (Hh) and Wingless-type (Wnt) pathways are associated with resistance to immune checkpoint inhibitors (ICIs) in preclinical studies. This study aimed assess the association between expression activation levels of Wnt Sonic (Shh) ICIs advanced NSCLC patients treated ICI. Hh was assessed by immunohistochemistry (Gli1 beta-catenin) on corresponding tumor tissues, plasma concentrations Shh (Wnt1, Wnt2 Wnt3) at ICI introduction first clinical evaluation. Sixty-three were included, 36...
The role of Notch signaling in acute myeloid leukemia (AML) is still under investigation. We have previously shown that high levels receptors and ligands could interfere with drug response. In this study, the protein expression 79 AML blast samples collected from newly diagnosed patients was examined through flow cytometry. Gamma-secretase inhibitors were used mouse xenograft models to evaluate contribution pharmacological inhibition survival. univariate analysis for testing correlation...
Wnt/β-catenin signaling has been reported in Acute Myeloid leukemia, but little is known about its significance as a prognostic biomarker and drug target. In this study, we first evaluated the correlation between expression levels of Wnt molecules clinical outcome. Then, studied—in vitro vivo—the anti-leukemic value combinatorial treatment inhibitors classic anti-leukemia drugs. Higher β-catenin, Ser675-phospho-β-catenin GSK-3α (total Ser 9) were found AML cells from intermediate or poor...
In order to investigate the role of microRNAs in pathogenesis different B-cell lymhoma subtypes, we have applied an array-based assay a series 76 mixed non-Hodgkin lymphomas, including Burkitt's lymphoma (BL), diffuse large lymphoma, primary mediastinal mantle cell (MCL) and follicular lymphoma. Lymphomas clustered according histological driven by two miRNA clusters (the miR-29 family miR-17-92 cluster). Since are known be MYC-regulated, investigated whether this would supported MYC-driven...
Protein tyrosine phosphatase receptor type γ (PTPRG) is a tumor suppressor gene, down-regulated in Chronic Myeloid Leukemia (CML) cells by the hypermethylation of its promoter region. β-catenin (CTNNB1) critical regulator Leukemic Stem Cells (LSC) maintenance and CML proliferation. This study aims to demonstrate antagonistic regulation between PTPRG cells. The specific inhibition increases activation state BCR-ABL1 modulates expression BCR-ABL1- downstream gene β-Catenin. was found be...
The identification of biomarkers related to treatment in patients with non-small cell lung cancer (NSCLC) treated immune checkpoint inhibitors (ICIs) represents a significant challenge. aim this study was determine the predictive value macrophage-related markers assessed plasma and tissue samples NSCLC undergoing ICI treatment. This bicentric included prospective cohort 88 advanced who received first-line therapy (either as monotherapy or combination chemotherapy) chemotherapy alone (CT)....
Immune checkpoint inhibitors (ICIs) are commonly used in patients with advanced non-small cell lung cancer (NSCLC). An unmet need remains for new biomarkers associated ICIs. In this study, consecutive NSCLC treated nivolumab or pembrolizumab were included. Plasma at ICIs initiation was prospectively collected and a multiplex ELISA assay testing 48 cytokines growth factors performed. Exploratory endpoints the association between plasma outcome grade III–IV immune related adverse events...
First line drug treatment of follicular lymphoma (FL) patients is followed by a highly variable disease-free time before relapse in about one third patients. No molecular marker able to predict efficiently the risk relapse. We investigated expression profile microRNAs (miRNAs) microarrays and tumor microenvironment immunohistochemistry 26 FLs 12 reactive lymph nodes (rLN) as reference. Twenty-nine miRNAs were differentially expressed compared rLNs some them discriminated grade 1 from 3a FLs....
Notch signaling contribution to B-cell acute lymphoblastic leukemia (B-ALL) development is still under investigation. The serendipitous onset of B-ALL in a patient affected by the germinal mutation-dependent Alagille syndrome allowed us establish cell line (VR-ALL) bearing genetic loss function components signaling. VR-ALL common-type line, grows conventional culture medium supplemented with 10% serum, and gives rise, once injected into immunodeficient NOG mice, mouse xenograft model B-ALL....