A5007160237- Prion Diseases and Protein Misfolding
- Neurological diseases and metabolism
- Trace Elements in Health
- Identification and Quantification in Food
- Pharmaceutical and Antibiotic Environmental Impacts
- Milk Quality and Mastitis in Dairy Cows
- Probiotics and Fermented Foods
- Metallurgy and Material Science
- Salmonella and Campylobacter epidemiology
- RNA regulation and disease
- Animal Disease Management and Epidemiology
- Food Safety and Hygiene
- Isotope Analysis in Ecology
- Selenium in Biological Systems
- European and International Contract Law
- Dye analysis and toxicity
- Yersinia bacterium, plague, ectoparasites research
- Neuroblastoma Research and Treatments
- Quantum Dots Synthesis And Properties
- Toxic Organic Pollutants Impact
- Olfactory and Sensory Function Studies
- Metabolomics and Mass Spectrometry Studies
- Glioma Diagnosis and Treatment
- Cancer-related molecular mechanisms research
- Microbial infections and disease research
Istituto Zooprofilattico Sperimentale del Piemonte Liguria e Valle d'Aosta
2011-2025
University of Naples Federico II
2020
Bovine spongiform encephalopathy (BSE), the prion disease in cattle, was widely believed to be caused by only one strain, BSE-C. BSE-C causes fatal named new variant Creutzfeldt-Jacob humans. Two atypical BSE strains, bovine amyloidotic (BASE, also BSE-L) and BSE-H, have been discovered several countries since 2004; their transmissibility phenotypes humans are unknown. We investigated infectivity human phenotype of BASE strains inoculating transgenic (Tg) mice expressing protein with brain...
Fish fraud detection is mainly carried out using a genomic profiling approach requiring long and complex sample preparations assay running times. Rapid evaporative ionisation mass spectrometry (REIMS) can circumvent these issues without sacrificing loss in the quality of results. To demonstrate that REIMS be used as fast technique capable achieving accurate species identification need for any preparation. Additionally, we wanted to other aspects fish than speciation are detectable REIMS. 478...
Susceptibility of sheep to scrapie, a transmissible spongiform encephalopathy small ruminants, is strongly influenced by polymorphisms the prion protein gene (PRNP). Breeding programs have been implemented increase scrapie resistance in populations; though desirable, similar approach has not yet applied goats. European studies now suggested that several can modulate susceptibility goats: particular, PRNP variant K222 associated with case-control Italy, France and Greece. In this study we...
The recombinant bovine somatotropin (rbST) is a synthetic hormone developed to mimic the effects of endogenous growth hormone, also known as (bST). Although rbST use in dairy cows authorized several countries, it currently banned Europe. Different methods for screening and confirmatory detection were developed, mainly based on LC-MS/MS immune-enzymatic assays. However, some commercial forms have above same amino acid sequence bST, making difficult produce reliable differentiation from forms....
ABSTRACT Tongue involvement by prion spreading was shown to be a common outcome after oral or intracranial experimental challenge with scrapie and transmissible mink encephalopathy sources in rodent models. It is also known that bovine spongiform encephalopathy, which pathogenic for humans, experimentally sheep can lead disease indistinguishable from scrapie. A recent European Food Safety Authority opinion recommended research into PrP sc accumulation the tongues of ruminants. We report on...
The olfactory system (OS) is involved in many infectious and neurodegenerative diseases, both human animal, it has recently been investigated regard to transmissible spongiform encephalopathies. Previous assessments of nasal mucosa infection by prions following intracerebral challenge suggested a potential centrifugal spread along the nerve fibers pathological prion protein (PrP(Sc)). Whether cavity may be route for centripetal brain also experimentally studied. With present study, we wanted...
Salmonella is the second most frequent bacterial pathogen involved in human gastrointestinal outbreaks European Union; it can enter food production chain from animal or environmental sources asymptomatic operators. legislation has established microbiological criteria to ensure consumer protection. listed under both process hygiene and safety criteria. Each Member State designates an agency organize perform controls other official activities. This paper describes control plans performed by...
Staphylococcal food poisoning results from the consumption of contaminated by staphylococcal enterotoxins. In July 2022, Turin local health board was notified a suspected foodborne outbreak involving six children who had consumed döner kebab purchased takeaway restaurant. The symptoms (vomiting and nausea) were observed 2–3 h later. A microbiological analysis samples revealed high levels (1.5 × 107 CFU/g) coagulase-positive staphylococci (CPS). immunoassay detected contamination with...
Antibiotic residues in milk are a serious health and technological problem dairy processes. This study aims to verify the absence of administered antimicrobials after therapeutic treatments, taking into consideration withdrawal period, evaluate reliability screening tests under field conditions confirmatory HPLC-HRMS (High Performance Liquid Chromatography-High-Resolution Mass Spectrometry) Orbitrap analysis. Moreover, presence expected or non-targeted metabolites was investigated using new...
Late blowing defects in semi-hard and hard cheeses caused by spore-forming clostridia (e.g.,
Salmonella is the second most frequent bacterial pathogen involved in human gastrointestinal outbreaks European Union; it can enter food-production chain from animal or environmental sources asymptomatic food operators. legislation has established microbiological criteria to ensure consumer protection.
Three distinct forms of bovine spongiform encephalopathy (BSE), defined as classical (C-), low (L-) or high (H-) type, have been detected through ongoing active and passive surveillance systems for the disease.The aim present study was to compare ability two sets immunohistochemical (IHC) Western blot (WB) BSE confirmatory protocols detect C- atypical (L- H-type) forms.Obex samples from cases United States Italian C-type BSE, a U.S. H-type an L-type case were tested in parallel using IHC WB...
Arcobacter spp. has been recognized as an emerging foodborne pathogen and a hazard to human health. In the dairy chain, it isolated from different sources, nevertheless data on occurrence in raw milk provided by vending machines are few. This study aimed identify potentially pathogenic intended for consumption sold through located Piedmont. 8-month period, 37 samples were collected 24 farms: 12 (32,4%) directly farm bulk tank 25 (67,6%) machines. Eight (21,6%) out of 7 (29,2%) farms positive...
To evaluate further the reactivity of prion-specific monoclonal antibodies containing 89–112 or 136–158 prion protein (PrP) polypeptides, immunoprecipitations were performed on brain extracts from Italian bovines, sheep and goats with transmissible spongiform encephalopathies. No binding IgG to PrP in normal was detected. Conversely, both reagents immunoprecipitated bovine amyloidotic encephalopathies, typical atypical scrapie extracts. The bands mirrored Western blot (WB) profile different...
Prion protein (PrP) is encoded by the PRNP gene, which highly polymorphic in goats, with polymorphisms encoding amino acid substitutions at level. In current study, reactivity of monoclonal antibody (mAb) F99/97.6.1 binding PrP from goats codon 222 was investigated. Nervous tissue 30 scrapie-negative 3 different genotypes (222Q/Q, 222Q/K, and 222K/K) analyzed Western blot using mAbs P4 F99/97.6.1. Although detected all samples mAb P4, detection limited to 222Q/Q (12/12). No 222K/K ( n = 6),...