A5048603885- Mass Spectrometry Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Advanced Proteomics Techniques and Applications
- Analytical Chemistry and Chromatography
- Advanced Chemical Sensor Technologies
- Ion-surface interactions and analysis
- Molecular Biology Techniques and Applications
- Cancer, Lipids, and Metabolism
- Nuclear Physics and Applications
- Analytical chemistry methods development
- Microfluidic and Capillary Electrophoresis Applications
- Bacterial Identification and Susceptibility Testing
- Cancer, Hypoxia, and Metabolism
- Spectroscopy Techniques in Biomedical and Chemical Research
- Gut microbiota and health
- SARS-CoV-2 and COVID-19 Research
- Cervical Cancer and HPV Research
- Amino Acid Enzymes and Metabolism
- Breast Lesions and Carcinomas
- Colorectal Cancer Screening and Detection
- Isotope Analysis in Ecology
- Antibiotic Resistance in Bacteria
- Bioinformatics and Genomic Networks
- Colorectal Cancer Surgical Treatments
- Radiomics and Machine Learning in Medical Imaging
Imperial College London
2016-2025
University of Regensburg
2024-2025
Rosalind Franklin Institute
2020-2025
Inserm
2018-2024
Protéomique, Réponse Inflammatoire et Spectrométrie de Masse
2020-2024
Université de Lille
2020-2024
St Mary's Hospital
2016-2023
International Severe Acute Respiratory and Emerging Infection Consortium
2023
The London College
2017-2022
Royal College of Obstetricians and Gynaecologists
2022
Rapid evaporative ionization mass spectrometry (REIMS) is an emerging technique that allows near-real-time characterization of human tissue in vivo by analysis the aerosol ("smoke") released during electrosurgical dissection. The coupling REIMS technology with electrosurgery for diagnostics known as intelligent knife (iKnife). This study aimed to validate applying it fresh samples ex and demonstrate translation real-time use a surgical environment. A variety from 302 patients were analyzed...
Desorption electrospray ionization (DESI) mass spectrometry is used to detect trace amounts of explosives present on a variety ambient surfaces in 5-second analysis times without any sample preparation.
Desorption electrospray ionization (DESI), an ambient mass spectrometry technique, is used for trace detection of the explosives trinitrohexahydro-1,3,5-triazine (RDX), octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (HMX), 2,4,6-trinitrotoluene (TNT), Pentaerythritol tetranitrate (PETN), and their plastic compositions (Composition C-4, Semtex-H, Detasheet) directly from a wide variety surfaces (metal, plastic, paper, polymer) without sample preparation or pretreatment. Analysis performed...
Desorption electrospray ionization (DESI) allows mass spectrometry to be used for on-line high-throughput monitoring of pharmaceutical samples in the ambient environment, without prior sample preparation. Positive and negative ion DESI are characterize active ingredients formulated as tablets, ointments, liquids. Compounds a wide variety chemical types detected these complex matrices. The effects on analytical performance operating parameters, including high voltage, heated capillary...
Beam me up: A novel mass spectrometric ionization technique based on rapid evaporation of biological tissues (see picture) can be used to analyze vital during surgical intervention as well for processed tissue specimens. identification system principal-component analysis was developed. The method differentiates malignant tumor cells from the surrounding healthy tissue.
A nanoparticle formulation of an Aurora B kinase inhibitor uses ion pairing to achieve controlled release and efficacious, nontoxic target inhibition in tumors.
Re-operation for positive resection margins following breast-conserving surgery occurs frequently (average = 20–25%), is cost-inefficient, and leads to physical psychological morbidity. Current margin assessment techniques are slow labour intensive. Rapid evaporative ionisation mass spectrometry (REIMS) rapidly identifies dissected tissues by determination of tissue structural lipid profiles through on-line chemical analysis electrosurgical aerosol toward real-time assessment....
Abstract Sustained smouldering, or low-grade activation, of myeloid cells is a common hallmark several chronic neurological diseases, including multiple sclerosis 1 . Distinct metabolic and mitochondrial features guide the activation diverse functional states 2 However, how these act to perpetuate inflammation central nervous system unclear. Here, using multiomics approach, we identify molecular signature that sustains microglia through complex I activity driving reverse electron transport...
Abstract Extracellular vesicles (EVs), including exosomes and microvesicles, mediate intercellular communication in cancer, from development to metastasis. EV‐based liquid biopsy is a promising strategy for cancer diagnosis as EVs can be found patients’ body fluids. In this study, the lipid composition of breast cancer‐derived was studied well potential blood plasma identification biomarkers detection. Initially, an untargeted lipidomic analysis carried out panel cancerous non‐cancerous...
Precise manipulation of flexible surgical tools is crucial in minimally invasive procedures, necessitating a miniature and robotic probe that can precisely direct the instruments. In this work, we developed polymer-based fiber with thermal actuation mechanism by local heating along sides single fiber. The robot was fabricated highly scalable drawing technology using common low-cost materials. This low-profile (below 2 millimeters diameter) exhibits remarkable motion precision 50 micrometers)...
Intact, multiply protonated proteins of particular mass and charge were selected from ionized protein mixtures gently landed at different positions on a surface to form microarray. An array cytochrome c, lysozyme, insulin, apomyoglobin was generated, the deposited showed electrospray ionization spectra that matched those authentic compounds. Deposited lysozyme trypsin retained their biological activity. Multiply charged ions kinase A catalytic subunit hexokinase also soft-landed into...
Electrosonic spray ionization (ESSI), a variant on electrospray (ESI), employs traditional micro ESI source with supersonic nebulizing gas. The high linear velocity of the gas provides efficient pneumatic spraying charged liquid sample. variable electrostatic potential can be tuned to allow and gentle ionization. This method is successfully applied aqueous solutions various proteins at neutral pH, its performance compared that nanospray techniques. Evidence for desolvation during ESSI...
Direct chemical profiling of biological tissues, including metastatic human-liver adenocarcinoma, was carried out by using mass spectrometry under ambient conditions. Desorption electrospray ionization revealed enhanced signals from sphingolipids and increased unsaturation levels the phospholipids in tumor region tissue (see picture).
ABC multidrug transporters (MDR-ABC proteins) cause multiple drug resistance in cancer and may be involved the decreased anti-cancer efficiency modified pharmacological properties of novel specifically targeted agents. It has been documented that ABCB1 ABCG2 interact with several first-generation, small-molecule, tyrosine kinase inhibitors (TKIs), including Bcr-Abl fusion inhibitor imatinib, used for treatment chronic myeloid leukaemia. Here, we have investigated specific interaction these...
Desorption electrospray ionization (DESI) mass spectrometry is applied to the in situ detection of alkaloids tissue poison hemlock (Conium maculatum), jimsonweed (Datura stramonium) and deadly nightshade (Atropa belladonna). The experiment carried out by electrospraying micro-droplets solvent onto native or freshly-cut plant surfaces. No sample preparation required spectra are recorded under ambient conditions, times a few seconds. impact sprayed droplets on surface produces gaseous ions...
The newly developed rapid evaporative ionization mass spectrometry (REIMS) provides the possibility of in vivo, situ spectrometric tissue analysis. experimental setup for REIMS is characterized detail first time, and description testing an equipment capable vivo analysis presented. spectra obtained by various standard surgical equipments were compared found highly specific to histological type tissues. based on their different phospholipid distribution; identification algorithm uses a...
Next-generation imaging mass spectrometry of biological tissue on the cellular level shows excellent agreement with histochemical evaluation whilst providing a much higher discrimination capacity based label-free molecular information (see image mouse urinary bladder section). Detailed facts importance to specialist readers are published as "Supporting Information". Such documents peer-reviewed, but not copy-edited or typeset. They made available submitted by authors. Please note: The...
Breast cancer is a heterogeneous disease characterized by varying responses to therapeutic agents and significant differences in long-term survival. Thus, there remains an unmet need for early diagnostic prognostic tools improved histologic characterization more accurate stratification personalized intervention. This study evaluated comprehensive metabolic phenotyping method breast tissue that uses desorption electrospray ionization mass spectrometry imaging (DESI MSI), both as novel tool...