A5038514497- Black Holes and Theoretical Physics
- Neuropeptides and Animal Physiology
- Receptor Mechanisms and Signaling
- Cosmology and Gravitation Theories
- Neuroscience of respiration and sleep
- Particle physics theoretical and experimental studies
- Quantum Chromodynamics and Particle Interactions
- Noncommutative and Quantum Gravity Theories
- Nicotinic Acetylcholine Receptors Study
- Nitric Oxide and Endothelin Effects
- Ion channel regulation and function
- Asthma and respiratory diseases
- Biochemical Analysis and Sensing Techniques
- Olfactory and Sensory Function Studies
- Pain Mechanisms and Treatments
- Ion Channels and Receptors
- Neonatal Respiratory Health Research
- Antenna Design and Optimization
- Quantum Electrodynamics and Casimir Effect
- Neuroscience and Neuropharmacology Research
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Antenna Design and Analysis
- High-Energy Particle Collisions Research
- Caveolin-1 and cellular processes
- Cancer, Hypoxia, and Metabolism
Justus-Liebig-Universität Gießen
2015-2024
Cardio-Pulmonary Institute
2009-2024
German Center for Lung Research
2013-2023
Universities of Giessen and Marburg Lung Center
2010-2020
National Institute of Mental Health
2018
Charité - Universitätsmedizin Berlin
2004-2015
Universitätsklinikum Gießen und Marburg
2004-2015
Institute of Pathology Celle
2006-2015
Giessen School of Theology
1999-2012
Institute of Cell Biology and Neurobiology
2000-2011
Antenna patterns with ultra-low sidelobes have been obtained for an experimental receiving array designed time-modulated slot radiators. The technique utilizes on-off RF switches which are programmed in a predetermined sequence to produce the desired pattern. results of tests on eight-element sidelobe reduction presented. Two examples considered: 1) initial static pattern 30-db reduced about 40 db by sequential switching and 2) 13-db directly different switching. Measured illustrating...
Nonphagocytic NADPH oxidases have recently been suggested to play a major role in the regulation of physiological and pathophysiological processes, particular, hypertrophy, remodeling, angiogenesis systemic circulation. Moreover, serve as oxygen sensors lung. Chronic hypoxia induces vascular remodeling with medial hypertrophy leading development pulmonary hypertension. We screened lung tissue for expression oxidase subunits. NOX1, NOXA1, NOXO1, p22phox, p47phox, p40phox, p67phox, NOX2, NOX4...
In the epithelium of lower airways, a cell type unknown function has been termed “brush cell” because distinctive ultrastructural feature, an apical tuft microvilli. Morphologically similar cells in nose have identified as solitary chemosensory responding to taste stimuli and triggering trigeminal reflexes. Here we show that brush mouse trachea express receptors (Tas2R105, Tas2R108), downstream signaling molecules (α-gustducin, phospholipase C β2 ) bitter transduction, synthesis packaging...
Substance P (SP), neurokinin A (NKA), and calcitonin gene-related peptide (CGRP) have potent proinflammatory effects in the airways. They are released from sensory nerve endings originating jugular dorsal root ganglia. However, major supply to airways originates nodose ganglion. In this study, we evaluated changes neuropeptide biosynthesis airway innervation of ovalbumin-sensitized -challenged guinea pigs at mRNA level. airways, a three- fourfold increase SP, NKA, CGRP, was seen 24 h...
1. The present study addressed the hypothesis that jugular and nodose vagal ganglia contain somata of functionally anatomically distinct airway afferent fibres. 2. Anatomical investigations were performed by injecting guinea‐pig airways with neuronal tracer Fast Blue. animals killed 7 days later, removed immunostained antisera against substance P (SP) neurofilament protein (NF). In ganglion, NF‐immunoreactive neurones accounted for about 98% Blue‐labelled cells while in ganglion they...
In airway epithelia, non-neuronal cholinergic regulations have been described; however, the route for acetylcholine (ACh) release has not verified. To investigate whether organic cation transporters (OCTs) serve this function, we studied expression of OCTs in epithelia and their capability to translocate ACh. Using immunohistochemistry rats humans, OCT1, OCT2, OCT3 were localized luminal membrane ciliated epithelial cells. OCT2 showed strongest membrane. We expressed OCT isoforms oocytes...
A growing number of TRP channels have been identified as key players in the sensation smell, temperature, mechanical forces and taste. TRPM5 is known to be abundantly expressed taste receptor cells where it participates sweet, amino acid bitter perception. role other sensory systems, however, has not studied so far.Here, we systematically investigated expression rat mouse tissues. Apart from buds, found predominantly localized on basolateral surface cells, immunoreactivity was seen...
Next-generation imaging mass spectrometry of biological tissue on the cellular level shows excellent agreement with histochemical evaluation whilst providing a much higher discrimination capacity based label-free molecular information (see image mouse urinary bladder section). Detailed facts importance to specialist readers are published as "Supporting Information". Such documents peer-reviewed, but not copy-edited or typeset. They made available submitted by authors. Please note: The...
Participation of nitric oxide (NO) in the autonomic innervation rat and guinea pig hearts was investigated by applying NADPH diaphorase technique immunohistochemistry with NO synthase antiserum. We present evidence that is localized cardiac ganglion cells nerve fibers innervating sinuatrial atrioventricular nodes, myocardium, local neurons, coronary arteries, pulmonary vessels, suggesting an involvement neurogenic heart rate regulation, myocardial cell function, neuronal transmission...
Complex regional pain syndrome (CRPS) is a painful condition affecting one or more extremities of the body, marked by wide variety symptoms and signs that are often difficult to manage because pathophysiology incompletely understood. Thus, diverse treatments might be ineffective. A recent report revealed presence autoantibodies against differentiated autonomic neurons in CRPS patients. However, it remained unclear how antibodies act development CRPS. We therefore aimed characterize these...
Chemosensory cells in the mucosal surface of respiratory tract ("brush cells") use canonical taste transduction cascade to detect potentially hazardous content and trigger local protective aversive reflexes on stimulation. So far, urogenital has been considered lack this cell type. Here we report presence a previously unidentified cholinergic, polymodal chemosensory mammalian urethra, potential portal entry for bacteria harmful substances into system, but not further centrally located parts...
Using a digital videomicroscopic analysis system in the bovine, we showed that mechanisms of transport caused by ciliary beating are distinctly different ampulla and isthmus oviduct. The average particle speed (PTS) oviduct (mean, 133 μm/sec) does not differ cycle (metestrus) during pregnancy after implantation, but it is locally modulated at site embryo. videomicroscopy, were able to document entering ampulla, cumulus-oocyte complex (COC) transported down oviduct, firmly attaches ampullar...
The mouse gastro-intestinal and biliary tract mucosal epithelia harbor choline acetyltransferase (ChAT)-positive brush cells with taste cell-like traits. With the aid of two transgenic lines that express green fluorescent protein (EGFP) under control ChAT promoter (EGFPChAT) by using in situ hybridization immunohistochemistry we found EGFPChAT were clustered epithelium lining gastric groove. numerous gall bladder bile duct, scattered as solitary along small large intestine. While all also...
Mucociliary clearance through coordinated ciliary beating is a major innate defense removing pathogens from the lower airways, but pathogen sensing and downstream signaling mechanisms remain unclear. We identified virulence-associated formylated bacterial peptides that potently stimulated ciliary-driven transport in mouse trachea. This response was independent of formyl peptide taste receptors depended on key transduction genes. Tracheal cholinergic chemosensory cells expressed these genes,...
Host-derived succinate accumulates in the airways during bacterial infection. Here, we show that luminal activates murine tracheal brush (tuft) cells through a signaling cascade involving receptor 1 (SUCNR1), phospholipase Cβ2, and cation channel transient potential subfamily M member 5 (TRPM5). Stimulated then trigger long-range Ca 2+ wave spreading radially over epithelium sequential process. First, release acetylcholine, which excites nearby via muscarinic acetylcholine receptors. From...
Production of nitric oxide (NO) in endothelial cells is regulated by direct interactions synthase (eNOS) with effector proteins such as Ca2+-calmodulin, posttranslational modifications phosphorylation via protein kinase B, and translocation the enzyme from plasma membrane caveolae to intracellular compartments. Reversible acylation eNOS thought contribute trafficking enzyme; however, factor(s) that govern are still unknown. Here we have used yeast two-hybrid system identified a novel 34 kDa...
In the pulmonary vasculature, mechanisms responsible for oxygen sensing and initiation of hypoxia-induced vasoconstriction vascular remodeling are still unclear. Nitric oxide (NO) reactive species (ROS) discussed as early mediators hypoxic response. Here, we describe a quantitative analysis NO- ROS-producing cells within walls murine lung sections cultured at normoxia or hypoxia. Whereas number NO-producing was not changed by hypoxia, ROS-generating significantly increased. Addition specific...