A5043082016- Nerve injury and regeneration
- Neuropeptides and Animal Physiology
- Fibroblast Growth Factor Research
- Neurogenesis and neuroplasticity mechanisms
- Axon Guidance and Neuronal Signaling
- Anesthesia and Sedative Agents
- Anesthesia and Pain Management
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Parkinson's Disease Mechanisms and Treatments
- Pain Mechanisms and Treatments
- Receptor Mechanisms and Signaling
- Anesthesia and Neurotoxicity Research
- Nerve Injury and Rehabilitation
- Botulinum Toxin and Related Neurological Disorders
- Kruppel-like factors research
- Cellular transport and secretion
- Neuroinflammation and Neurodegeneration Mechanisms
- Neuroscience and Neuropharmacology Research
- Ubiquitin and proteasome pathways
- Neuroscience of respiration and sleep
- Hippo pathway signaling and YAP/TAZ
- Pancreatic function and diabetes
- Regulation of Appetite and Obesity
- Cancer-related gene regulation
- Genetics and Neurodevelopmental Disorders
Innsbruck Medical University
2016-2025
Universität Innsbruck
2014-2024
Nathan Kline Institute for Psychiatric Research
2007
Institute of Neuroimmunology of the Slovak Academy of Sciences
2006
Columbia University
2000
Heidelberg University
1992-1999
GTx (United States)
1997
Eppendorf (Germany)
1992
Abstract Alpha‐synucleinopathies (ASP) are neurodegenerative disorders, characterized by accumulation of misfolded α‐synuclein, selective neuronal loss, and extensive gliosis. It is accepted that microgliosis astrogliosis contribute to the disease progression in ASP. Toll‐like receptors (TLRs) expressed on cells innate immune system, including glia, TLR4 dysregulation may play a role ASP pathogenesis. In this study we aimed define involvement microglial astroglial activation induced...
Participation of nitric oxide (NO) in the autonomic innervation rat and guinea pig hearts was investigated by applying NADPH diaphorase technique immunohistochemistry with NO synthase antiserum. We present evidence that is localized cardiac ganglion cells nerve fibers innervating sinuatrial atrioventricular nodes, myocardium, local neurons, coronary arteries, pulmonary vessels, suggesting an involvement neurogenic heart rate regulation, myocardial cell function, neuronal transmission...
Functional recovery and regeneration of corticospinal tract (CST) fibers following spinal cord injury by compression or dorsal hemisection in mice was monitored after application the enzyme-deficient Clostridium botulinum C3-protein-derived 29-amino-acid fragment C3bot(154-182). This peptide significantly improved locomotor restoration both models as assessed open-field Basso Mouse Scale for locomotion test Rotarod treadmill experiments. These data were supported tracing studies showing an...
Animal models show us that specific activation of the p38 mitogen-activated protein kinase (MAPK) may be a pivotal step in lidocaine neurotoxicity, but this has not been investigated case two very widely used local anesthetics, bupivacaine and ropivacaine. We hypotheses these drugs (A) are less neurotoxic than prototype anesthetic, (B) selectively toxic for subcategories dorsal root ganglion neurons (C) induce either MAPK or related enzymes, such as c-jun terminal N-kinase (JNK)...
Adult neurogenesis has been implicated in affective disorders and the action of antidepressants (ADs) although functional significance this association is still unclear. The use animal models closely mimicking human comorbid anxiety seen majority patients should provide relevant novel information. Here, we used a unique genetic mouse model displaying higher trait (HAB) depression-like behavior. We demonstrate that HABs have lower rate hippocampal impaired integration newly born neurons as...
Neurotrophic factors such as nerve growth factor (NGF) promote a wide variety of responses in neurons, including differentiation, survival, plasticity, and repair. Such actions often require changes gene expression. To identify the regulated genes thereby to more fully understand NGF mechanism, we carried out serial analysis expression (SAGE) profiling transcripts derived from rat PC12 cells before after NGF-promoted neuronal differentiation. Multiple criteria supported reliability profile....
alpha-Synuclein is present in intracellular protein aggregates that are hallmarks of common neurodegenerative disorders including Parkinson disease, dementia with Lewy bodies, and multiple system atrophy. localized neurons presynaptic terminals. Under pathological conditions, however, it also found glia. The role alpha-synuclein glial cells its relevance to the molecular pathology diseases presently unclear. To investigate consequence overexpression glia, we transfected U373 astrocytoma...
Abstract The neuropeptides galanin and pituitary adenylate cyclase‐activating peptide (PACAP) are markedly up‐regulated in response to peripheral nerve lesion. Both peptides involved neuronal differentiation neurite outgrowth during development. In this study, we investigated the effects of PACAP on axonal elongation sprouting by adult rat sensory neurones vitro facial motor vivo . Dissociated dorsal root ganglion were plated laminin substrate analysed morphometrically. mean length number...
The rising incidence of ischemic stroke poses significant health and healthcare burdens. Given the limitations current therapeutic options, there is increasing interest in exploring potential galangin, a natural flavonoid compound, as treatment for stroke. This study aimed to evaluate neuroprotective effects underlying mechanisms galangin mitigating oxidative stress, inflammation, apoptosis rat model permanent cerebral ischemia. Sixty male Wistar rats were divided into six groups: control;...
Local anesthetic-induced direct neurotoxicity (paresthesia, failure to regain normal sensory and motor function) is a potentially devastating complication of regional anesthesia. anesthetics activate the p38 mitogen-activated protein kinase (MAPK) system, which involved in apoptotic cell death. The authors therefore investigated vitro (cultured primary neurons) vivo (sciatic nerve block model) potential neuroprotective effect MAPK inhibitor SB203580 administered together with clinical...
Pharmacologic inhibition of the p38 mitogen-activated protein kinase (MAPK) leads to a reduction in lidocaine neurotoxicity vitro and vivo. The current study investigated hypotheses that is specific for dorsal root ganglion cells different size or phenotype, involves time-dependent activation MAPK, MAPK inhibitors are only effective if applied with local anesthetic, triggers lipoxygenase pathways.The authors used primary sensory neuron cultures pheochromocytoma cell line detect related...
<h3>Background and Objectives</h3> Application of local anesthetics may lead to nerve damage. Increasing evidence suggests that risk neurotoxicity is higher in patients with diabetic peripheral neuropathy. In addition, block duration be prolonged We sought investigate vitro vivo a genetic animal model diabetes mellitus type 2. <h3>Methods</h3> the first experiments, neurons harvested from control Zucker fatty (ZDF) rats were exposed acute (24 hours) or chronic (72 hyperglycemia, followed by...
ABSTRACT Sprouty (Spry) proteins are negative feedback inhibitors of receptor tyrosine kinase signaling. Downregulation Spry2 has been demonstrated to promote elongative axon growth cultured peripheral and central neurons. Here, we analyzed global knockout mice with respect outgrowth in vitro regeneration vivo . Neurons dissociated from adult deficient sensory ganglia revealed stronger extracellular signal‐regulated activation enhanced outgrowth. Prominent elongation was observed...
Sprouty2 (SPRY2), a feedback regulator of receptor tyrosine kinase (RTK) signaling, has been shown to be associated with drug resistance and cell proliferation in glioblastoma (GBM), but the underlying mechanisms are still poorly defined.SPRY2 expression survival patterns patients gliomas were analyzed using publicly available databases. Effects RNA interference targeting SPRY2 on cellular established GBM or patient-derived stemlike cells examined. Loss- gain-of-function regulate tumorigenic...
The blocking of specific protein-protein interactions using nanoparticles is an emerging alternative to small molecule-based therapeutic interventions. However, the designed as “artificial proteins” generally require modification their surface with (bio)organic molecules and/or polymers ensure selectivity and specificity action. Here, we show that nanosized diamond crystals (nanodiamonds, NDs) without any synthetically installed interface enable efficient targeting family extracellular...