A5088669496- Listeria monocytogenes in Food Safety
- Microbial Inactivation Methods
- Essential Oils and Antimicrobial Activity
- Salmonella and Campylobacter epidemiology
- Genomics and Phylogenetic Studies
- Probiotics and Fermented Foods
- Antimicrobial Resistance in Staphylococcus
- Gut microbiota and health
- Streptococcal Infections and Treatments
- Antibiotic Resistance in Bacteria
- Bacteriophages and microbial interactions
- Escherichia coli research studies
- Bacterial Identification and Susceptibility Testing
- Infective Endocarditis Diagnosis and Management
- Bacterial biofilms and quorum sensing
- Clostridium difficile and Clostridium perfringens research
- RNA and protein synthesis mechanisms
- Vibrio bacteria research studies
- Viral gastroenteritis research and epidemiology
- Oral microbiology and periodontitis research
- Enterobacteriaceae and Cronobacter Research
- Neonatal Respiratory Health Research
- Bacterial Genetics and Biotechnology
- Pneumonia and Respiratory Infections
- Otolaryngology and Infectious Diseases
Justus-Liebig-Universität Gießen
2016-2025
German Center for Infection Research
2016-2025
Giessen School of Theology
2011-2022
Heinrich Heine University Düsseldorf
2021
Philipps University of Marburg
2018
Centers for Disease Control and Prevention
2018
Institute of Medical Microbiology and Hygiene
2001-2011
Universitat Autònoma de Barcelona
2010
Centre de Recerca en Sanitat Animal
2010
Institute for Laser Technology in Medicine and Measurement Technique
2002
Listeria monocytogenes is a food-borne pathogen with high mortality rate that has also emerged as paradigm for intracellular parasitism. We present and compare the genome sequences of L. (2,944,528 base pairs) nonpathogenic species, innocua (3,011,209 pairs). found large number predicted genes encoding surface secreted proteins, transporters, transcriptional regulators, consistent ability both species to adapt diverse environments. The presence 270 149 strain-specific (clustered in 100 63...
Listeria monocytogenes is a gram-positive, food-borne microorganism responsible for invasive infections with high overall mortality. L. among the very few microorganisms that can induce uptake into host cell and subsequently enter cytosol by breaching vacuolar membrane. We infected murine macrophage line P388D1 strain EGD-e examined gene expression profile of inside cytosolic environments using whole-genome microarray mutant analyses. found approximately 17% total genome was mobilized to...
Listeria monocytogenes is a food-borne pathogen that the causative agent of human listeriosis, an opportunistic infection primarily infects pregnant women and immunologically compromised individuals. Rapid, accurate discrimination between strains essential for appropriate therapeutic management timely intervention control. A rapid method involving matrix-assisted laser desorption ionization-time flight mass spectrometry (MALDI-TOF MS) shows promise identification species typing even allows...
ABSTRACT For nearly 3 decades, listeriologists and immunologists have used mainly three strains of the same serovar (1/2a) to analyze virulence bacterial pathogen Listeria monocytogenes . The genomes two these strains, EGD-e 10403S, were released in 2001 2008, respectively. Here we report genome sequence third reference strain, EGD, extensive genomic phenotypic comparisons strains. Strikingly, is genetically highly distinct from EGD (29,016 single nucleotide polymorphisms [SNPs]) 10403S...
Listeria monocytogenes is an important food-borne pathogen and model organism for host-pathogen interaction, thus representing invaluable target considering research on the forces governing evolution of such microbes. The diversity this species has not been exhaustively explored yet, as previous efforts have focused analyses serotypes primarily implicated in human listeriosis. We conducted complete genome sequencing 11 strains employing 454 GS FLX technology, thereby achieving full coverage...
Plasmids are extrachromosomal genetic elements that replicate independently of the chromosome and play a vital role in environmental adaptation bacteria. Due to potential mobilization or conjugation capabilities, plasmids important vehicles for antimicrobial resistance genes virulence factors with huge increasing clinical implications. They therefore subject large genomic studies within scientific community worldwide. As result rapidly improving next-generation sequencing methods, quantity...
Essential aspects of the innate immune response to microbial infection are conserved between insects and mammals. This has generated interest in using as model organisms study host-microbe interactions. We used greater wax moth Galleria mellonella, which can be reared at 37 degrees C, a host for examining virulence potential Listeria spp. Here we report that is an excellent surrogate listerial septic infection, capable clearly distinguishing pathogenic nonpathogenic strains even virulent...
Corynebacterium jeikeium is a "lipophilic" and multidrug-resistant bacterial species of the human skin flora that has been recognized with increasing frequency as serious nosocomial pathogen. Here we report genome sequence clinical isolate C. K411, which was initially recovered from axilla bone marrow transplant patient. The K411 consists circular chromosome 2,462,499 bp 14,323-bp bacteriocin-producing plasmid pKW4. contains 2,104 predicted coding sequences, 52% were considered to be...
The gene encoding the alternative sigma factor sigma(B) in Listeria monocytogenes is induced upon exposure of cells to several stresses. In this study, we investigated impact a sigB null mutation on survival L. EGD-e at low pH, during high-hydrostatic-pressure treatment, and freezing. Delta mutant exponential-phase pH 2.5 was 10,000-fold lower than wild-type cells. Moreover, failed show an acid tolerance response. Upon preexposure for 1 h 4.5, 100,000-fold wild type. glutamate decarboxylase...
Summary Pathogenic bacteria have to cope with defence mechanisms mediated by adaptive and innate immunity of the host cells. Cationic antimicrobial peptides (CAMPs) represent one most effective components immune response. Here we establish function Lmo1695, a member VirR‐dependent virulence regulon, recently identified in Listeria monocytogenes . Lmo1695 encodes membrane protein 98 kDa strong homology multiple peptide resistance factor (MprF) Staphylococcus aureus Like staphylococcal MprF,...
Listeria monocytogenes causes rhombencephalitis in humans and animals also affects the fetus utero , causing disseminated sepsis. In both instances, infection occurs by crossing of endothelial cells lining a physiological barrier, blood–brain barrier or transplacental barrier. this study, ability L. wild‐type EGD to invade human umbilical vein (HUVECs) was evaluated using bacteria isogenic mutants. Here, we show that invasion HUVECs is dependent on expression internalin B gene product. This...
Abstract Background Black elderberries ( Sambucus nigra L.) are well known as supportive agents against common cold and influenza. It is further that bacterial super-infection during an influenza virus (IV) infection can lead to severe pneumonia. We have analyzed a standardized elderberry extract (Rubini, BerryPharma AG) for its antimicrobial antiviral activity using the microtitre broth micro-dilution assay three Gram-positive bacteria one Gram-negative responsible infections of upper...
Small non-coding RNAs (sRNAs) are widespread effectors of post-transcriptional gene regulation in bacteria. Currently extensive information exists on the sRNAs Listeria monocytogenes expressed during growth extracellular environments. We used deep sequencing cDNAs obtained from fractioned RNA (<500 nt) isolated extracellularly growing bacteria and L. infected macrophages to catalog sRNA repertoire intracellular bacterial growth. Here, we report discovery 150 putative regulatory which 71 have...
The opportunistic food-borne gram-positive pathogen Listeria monocytogenes can exist as a free-living microorganism in the environment and grow cytoplasm of vertebrate invertebrate cells following infection. general stress response, controlled by alternative sigma factor, σB, has an important role for bacterial survival both during We used quantitative real-time PCR analysis immuno-blot to examine σB expression growth L. EGD-e. Whole genome-based transcriptional profiling was identify...
Klebsiella pneumoniae strains expressing ESBLs are a predominant cause of hospital acquired infections. Here we describe the molecular epidemiology these isolates in tertiary Tanzania, as potential pathogens for neonatal infections.Between April 2009 and March 2010 all with phenotypic expression Extended Spectrum Beta Lactamase (ESBL) were collected characterized. Identification was done using house biochemical tests case ambiguous results confirmation API 20E. Susceptibility testing...
Coronaviruses (CoVs) are important human pathogens for which no specific treatment is available. Here, we provide evidence that pharmacological reprogramming of ER stress pathways can be exploited to suppress CoV replication. The inducer thapsigargin efficiently inhibits coronavirus (HCoV-229E, MERS-CoV, SARS-CoV-2) replication in different cell types including primary differentiated bronchial epithelial cells, (partially) reverses the virus-induced translational shut-down, improves...
Mucociliary clearance through coordinated ciliary beating is a major innate defense removing pathogens from the lower airways, but pathogen sensing and downstream signaling mechanisms remain unclear. We identified virulence-associated formylated bacterial peptides that potently stimulated ciliary-driven transport in mouse trachea. This response was independent of formyl peptide taste receptors depended on key transduction genes. Tracheal cholinergic chemosensory cells expressed these genes,...
Whole genome sequencing of bacteria has become daily routine in many fields. Advances DNA technologies and continuously dropping costs have resulted a tremendous increase the amounts available sequence data. However, comprehensive in-depth analysis resulting data remains an arduous time-consuming task. In order to keep pace with these promising but challenging developments transform raw into valuable information, standardized analyses scalable software tools are needed. Here, we introduce...
Ulcerative colitis (UC) is a severe inflammatory bowel disease (IBD) characterized by multifactorial complex disorders triggered environmental factors, genetic susceptibility, and also gut microbial dysbiosis. Faecalibacterium prausnitzii, Bacteroides faecis, Roseburia intestinalis are underrepresented species in UC patients, leading to the hypothesis that therapeutic application of those bacteria could ameliorate clinical symptoms severity. Acute was induced mice 3.5% DSS, commensal...
ABSTRACT Renal transplant recipients are predisposed to urinary tract infections caused by both common uropathogens and opportunistic bacteria resulting frequently in significant polymicrobial infections. In this study, a culture-independent 16S rRNA-based approach was established identify unusual, fastidious, or anaerobic investigate bacterial diversity specimens. Similarly sized amplicons encompassing the V6 V8 region of rRNA were analyzed with denaturing high-performance liquid...