A5044594852- Viral-associated cancers and disorders
- Cytomegalovirus and herpesvirus research
- Polyomavirus and related diseases
- Cancer Genomics and Diagnostics
- Full-Duplex Wireless Communications
- Herpesvirus Infections and Treatments
- Plant Virus Research Studies
- HIV Research and Treatment
- SARS-CoV-2 and COVID-19 Research
- RNA modifications and cancer
- Animal Virus Infections Studies
- MicroRNA in disease regulation
- Viral gastroenteritis research and epidemiology
- Antenna Design and Analysis
- Bacteriophages and microbial interactions
- Epigenetics and DNA Methylation
- Animal Disease Management and Epidemiology
- Cancer-related molecular mechanisms research
- Respiratory viral infections research
- Genomics and Chromatin Dynamics
- Biofuel production and bioconversion
- Anaerobic Digestion and Biogas Production
- Immune Cell Function and Interaction
- Microbial metabolism and enzyme function
- RNA Research and Splicing
Leibniz Institute of Virology (LIV)
2016-2025
German Center for Infection Research
2014-2024
Institute of Virology of the Slovak Academy of Sciences
2024
Heinrich Heine University Düsseldorf
2021
Peter MacCallum Cancer Centre
2020
The University of Melbourne
2020
Universität Hamburg
2006-2017
University of Veterinary Medicine Hannover, Foundation
2015-2017
University Medical Center Hamburg-Eppendorf
2009-2017
Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche
2017
Fast and reliable detection of patients with severe heterogeneous illnesses is a major goal precision medicine1,2. Patients leukaemia can be identified using machine learning on the basis their blood transcriptomes3. However, there an increasing divide between what technically possible allowed, because privacy legislation4,5. Here, to facilitate integration any medical data from owner worldwide without violating laws, we introduce Swarm Learning-a decentralized machine-learning approach that...
We have developed an approach to identify microRNAs (miRNAs) that is based on bioinformatics and array-based technologies, without the use of cDNA cloning. The approach, designed for genomes small size (<2 Mb), was tested cells infected by either two lymphotropic herpesviruses, KSHV EBV. viral were scanned computationally pre-miRNAs using algorithm (VMir) we developed. Candidate hairpins suggested this analysis then synthesized as oligonucleotides microarrays, arrays hybridized with RNAs...
Abstract Here, as part of the Pan-Cancer Analysis Whole Genomes (PCAWG) Consortium, for which whole-genome and—for a subset—whole-transcriptome sequencing data from 2,658 cancers across 38 tumor types was aggregated, we systematically investigated potential viral pathogens using consensus approach that integrated three independent pipelines. Viruses were detected in 382 genome and 68 transcriptome datasets. We found high prevalence known tumor-associated viruses such Epstein–Barr virus...
Herpesvirus latency is generally thought to be governed by epigenetic modifications, but the dynamics of viral chromatin at early timepoints latent infection are poorly understood. Here, we report a comprehensive spatial and temporal analysis DNA methylation histone modifications during with Kaposi Sarcoma-associated herpesvirus (KSHV), etiologic agent Sarcoma primary effusion lymphoma (PEL). By use high resolution tiling microarrays in conjunction immunoprecipitation methylated (MeDIP) or...
We describe a multifactorial investigation of SARS-CoV-2 outbreak in large meat processing complex Germany. Infection event timing, spatial, climate and ventilation conditions the plant, sharing living quarters transport, viral genome sequences were analyzed. Our results suggest that single index case transmitted to co-workers over distances more than 8 m, within confined work area which air is constantly recirculated cooled. Viral sequencing shows all cases share set mutations representing...
Extrapulmonary manifestations of COVID-19 have gained attention due to their links clinical outcomes and potential long-term sequelae1. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) displays tropism towards several organs, including the heart kidney. Whether it also directly affects liver has been debated2,3. Here we provide clinical, histopathological, molecular bioinformatic evidence for hepatic SARS-CoV-2. We find that injury, indicated by a high frequency abnormal function...
Kaposi sarcoma-associated (KS-associated) herpesvirus (KSHV) infection is linked to the development of both KS and several lymphoproliferative diseases. In all cases, resulting tumor cells predominantly display latent viral infection. tumorigenesis requires ongoing lytic replication as well, however, for reasons that are unclear but have been suggested involve production angiogenic or mitogenic factors by lytically infected cells. Here we demonstrate proliferating with KSHV in vitro a marked...
Kaposi sarcoma–associated (KS-associated) herpesvirus (KSHV) infection is linked to the development of both KS and several lymphoproliferative diseases. In all cases, resulting tumor cells predominantly display latent viral infection. tumorigenesis requires ongoing lytic replication as well, however, for reasons that are unclear but have been suggested involve production angiogenic or mitogenic factors by lytically infected cells. Here we demonstrate proliferating with KSHV in vitro a marked...
West Nile virus (WNV) belongs to a group of medically important single-stranded, positive-sense RNA viruses causing deadly disease outbreaks around the world. The 3′ untranslated region (3′-UTR) flavivirus genome, in particular terminal stem–loop (3′SL) fulfils multiple functions replication and virus–host interactions. Using Kunjin strain WNV (WNVKUN), we detected virally encoded small RNA, named KUN-miR-1, derived from 3′SL. Transcription WNVKUN pre-miRNA mosquito cells either plasmid or...
Cellular and viral microRNAs (miRNAs) are involved in many different processes of key importance more than 10,000 miRNAs have been identified so far. In general, relatively little is known about their biological functions mammalian cells because phenotypic effects often mild targets still await identification. The recent discovery that Epstein-Barr virus (EBV) other herpesviruses produce own, barely conserved sets suggests these viruses usurp the host RNA silencing machinery to advantage...
The estrogen receptor α (ERα) controls cell proliferation and tumorigenesis by recruiting various cofactors to response elements (EREs) control gene transcription. A deeper understanding of these transcriptional mechanisms may uncover therapeutic targets for ERα-dependent cancers. We show that BRD4 regulates ERα-induced expression affecting elongation-associated phosphorylation RNA polymerase II (RNAPII) histone H2B monoubiquitination. Consistently, activity is required ER+ breast...
The diverse microbial communities in agricultural biogas fermenters are assumed to be well adapted for the anaerobic transformation of plant biomass methane. Compared natural systems, reactors limited their hydrolytic potential. reasons this not understood. In paper, we show that a typical industrial reactor fed with maize silage, cow manure, and chicken manure has relatively lower hydrolysis rates compared feces samples from herbivores. We provide evidence on average, 2.5 genes encoding...
Photobioreactors (PBRs) are very attractive for sunlight-driven production of biofuels and capturing anthropogenic CO2. One major problem associated with PBRs however, is that the bacteria usually microalgae in nonaxenic cultures can lead to biofouling thereby affect algal productivity. Here, we report on a phylogenetic, metagenome, functional analysis mixed-species bacterial biofilm Chlorella vulgaris Scenedesmus obliquus PBR. The diversity population dynamics were examined through 16S rRNA...
SummaryKaposi sarcoma (KS), an angioproliferative disease associated with Kaposi herpesvirus (KSHV) infection, harbors a diversity of cell types ranging from endothelial to mesenchymal cells unclear origin. We developed three-dimensional model for KSHV infection and used it demonstrate that induces transcriptional reprogramming lymphatic via endothelial-to-mesenchymal transition (EndMT). KSHV-induced EndMT was initiated by the viral proteins vFLIP vGPCR through Notch pathway activation,...
Pestiviruses are highly variable RNA viruses belonging to the continuously growing family Flaviviridae. A genetically very distinct pestivirus was recently discovered in USA, designated atypical porcine (APPV). Here, a screening of 369 sera from apparently healthy adult pigs demonstrated existence APPV Germany with an estimated individual prevalence 2.4% and ~10% at farm level. Additionally, genomes were detected newborn piglets affected by congenital tremor (CT), but absent unaffected...
ABSTRACT Interference with tumor suppressor pathways by polyomavirus-encoded antigens (T-Ags) can result in transformation. Consequently, it is thought that T-Ags encoded Merkel cell polyomavirus (MCPyV), a virus integrated ∼90% of all carcinoma (MCC) cases, are major contributors to tumorigenesis. The MCPyV large T-Ag (LT-Ag) has preserved the key functional domains present family members but also acquired unique regions flank LxCxE motif. As these may mediate functions, or modulate those...
Abstract Current antiviral therapies cannot cure hepatitis B virus (HBV) infection; successful HBV eradication would require inactivation of the viral genome, which primarily persists in host cells as episomal covalently closed circular DNA (cccDNA) and, to a lesser extent, chromosomally integrated sequences. However, novel designer enzymes, such CRISPR/Cas9 RNA-guided nuclease system, provide technologies for developing advanced therapy strategies that could directly attack genome. For...
Microalga are of high relevance for the global carbon cycling and it is well-known that they associated with a microbiota. However, remains unclear, if microbiota, often found in phycosphere biofilms, specific microalga strains which role individual bacterial taxa play. Here we provide experimental evidence Chlorella saccharophila, Scenedesmus quadricauda, Micrasterias crux-melitensis, maintained strain collections, unique microbial populations. Deep metagenome sequencing, binning...
The biological relevance of extracellular vesicles (EV) in intercellular communication has been well established. Thus far, proteins and RNA were described as main cargo. Here, we show that EV released from human bone marrow derived mesenchymal stromal cells (BM-hMSC) also carry high-molecular DNA addition. Extensive characterization revealed this mainly associated with the outer membrane to a smaller degree inside EV. Our purification protocol secured is not apoptotic or necrotic cells. To...